• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Beet Lab. Aim: To determine the effects of temperature on the permeability of the beetroot cell membrane.

Extracts from this document...

Introduction

Biology HL Lab Report Beet Lab Data Collection, Processing, Presentation Conclusion & Evaluation -Rashika Singh IB Yr 1 Aim: To determine the effects of temperature on the permeability of the beetroot cell membrane. Research Question: To investigate how different temperatures affect the beetroot pigment and finding out the absorbance and transmittance % using a visible spectrophotometer. Background Information: Beetroots contain betalains which are the red pigments present in the cell vacuole. Betalains are soluble in water and they contain nitrogen. Betalains extracted from beetroot is commonly used as food dye because it is not known to cause any allergic reactions. Cell membrane is the barrier that separates the inner environment of the cell from the outer environment. The membrane is selectively permeable. The cell membrane is made up of mainly lipids, carbohydrates and proteins. The lipids are the phospholipid bilayer which consists of a hydrophilic polar head and a hydrophobic tail. Among the proteins present in the cell membranes are integral proteins and peripheral proteins. {1} Lipids increase in fluidity as temperature increases. Once denatured, proteins start to unravel and are unable to carry out their function. Diffusion is the movement of molecules from high concentration to a low concentration down a concentration gradient. Facilitated diffusion and active transport requires assistance from proteins imbedded in the cell membrane to transport substances in and out of the cell. Any damage to the cell membrane will cause the cytoplasm to leak out of the cell. ...read more.

Middle

This may have an effect on the actual temperatures inside them and could mean a deviation from the originally planned temperatures. Apparatus: 1. Spectrophotometer 2. 5 test tubes 3. 5 water baths (20oC, 30oC, 40oC, 50oC, 60oC) 4. Hot water 5. Ice 6. Labels 7. Stopwatch 8. Glass stirring rod 1. Mounting needle/forceps to handle beetroot cores 2. Syringe 1. Thermometers for each water bath 2. 5 cuvettes Procedure: 1. Obtain the beetroot cores from the lab technician. 2. We put each of the beetroot cores in each of test tubes. 3. Using a syringe, we fill up 10 ml of water and pour it into the test tubes. 4. Meanwhile prepare the water baths (20oC, 30oC, 40oC, 50oC, 60oC). 5. When the water baths are ready, place each test tube in each of the water baths. Have your stopwatches ready. 6. Keep the test tubes for exactly 10 minutes in the water baths. 7. After 10 minutes, take out the test tubes, discard the beetroot cores and pour out its contents, the pigmented water, into the cuvette. 8. Ask the lab technician to help you calibrate and warm up the spectrophotometer to 480.5nm 9. Using one cuvette for each trial, measure out the absorbance and transmittance %. 10. Repeat steps 1-9 for all trials. 11. Record your observations in a table. Precautions: 1. Make sure you don?t spill out anything. 2. Beware of the hot water baths; use a test tube holder to take out the test tube from the hot water baths. ...read more.

Conclusion

2. Inability to maintain temperature of water baths Many times we were unable to follow the temperature trail, which would result in a varied membrane absorbance, with the temperature drop when all test tubes were put in the water baths, hence giving us inaccurate results. 3. Spilling of liquids Most if the coloured pigment water may spill out, hence not giving us the respective colours, and will result in inaccurate spectrophotometer readings which acts as major error instigator. Be more careful in handling the liquids to prevent spilling of anything. If anything does spill, redo the entire experiment again. 4.Timekeeping The test tubes of beetroot cores were places a little too early or a little too late. As the amount of time increases, the amount of diffused pigment will also increase. Overshooting of time for the test tubes in the water baths will have resulted in accumulated errors. Coordinate efforts and place the test tubes in the water baths at the same time to reduce errors as mentioned above. Stay to your time limit, and never overshoot the time. 5. Confusion to procedure Many times confusion in procedure will contribute lots of errors to the readings. Come prepared and read the procedure and precautions before you start the lab. If there is any confusion, do not hesitate to ask the lab assistant. 6. Large margins between different trials 7. The pH of the water Most of the times tap water is used, and tap water contains a lot of impurities, which affect the permeability of the membrane. Use of distilled water to prevent impurities that might alter the pH. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Biology HL IA - Transpiration lab report

    but covered with an oily material, for instance, nail polish. This would work as stomata are usually located on the lower and sometimes upper epidermis of leaves surface and nail polish would cover them. Second, as the area of leaves reduced every time depended on leaves' shape, it was difficult

  2. Biology Lab Report-Osmosis

    Table 5: Results for Trial 3. Solution Mass of chip- (in g �0.01g) Percentage change in mass (in %) Length of chip- (in mm �0.5mm) Percentage change in length (in %)

  1. Biology Lab Report - Effects of Mouthwash on Oral Bacteria

    4. When finished, give your sealed agar plate to the teacher for disposal. 6. Data Collection and Processing ii. Observations Chart Observations - Put your money where your mouth is... Type of Mouthwash Zone of Inhibition #1 Zone of Inhibition #2 Zone of Inhibition #3 Zone of Inhibition #4 Average Zone of Inhibition A.

  2. The Effects of Salinity on Wheat Germination

    0% Salt Solution 2% Salt Solution 5% Salt Solution Mean 1.111111 Mean 0.888889 Mean 0.555556 Graph Growth per day � 0.5 mm (possible to discern down to the 0.25 of a mm) Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Day 8 Day 9

  1. Beetroot Plasma Investigation. The main objective of this investigation is to focus on ...

    This will therefore lead to more leakage of the cells fluid to the outside, resulting in more red pigment to leave the cell. This will tinge the solution and so less light will be able to pass through in the colorimeter.

  2. Vitamin C Lab

    Gather and set up all equipment as shown 2. Label one 500cm3 beaker, "waste" 3. Label the other 500cm3 beaker, "lemon juice" 4. Pour some DCPIP from the big jug into 100cm3 beaker 5. Pour DCPIP from the 100cm3 beaker into a funnel into the burette, with the DCPIP just over the 0.00cm3 reading 6.

  1. Biological Membrane Lab

    Materials/Equipment: See Lab Handout Procedure: See Lab Handout RESULTS Data Collection: Table 1: The amount of damage various alcohols cause to cell membranes Trial Concentration (%) Absorbance Methanol Ethanol 1-Propanol 1 0 0.061 0.062 0.060 2 10 0.068 0.074 0.088 3 20 0.069 0.100 0.090 4 30 0.071 0.068 0.059 5 40 0.079 0.237 0.694 Fig.

  2. Effect of Detergent on Membrane Permeability

    If the percent increment of detergent increases when applied on the beetroot, the light absorption rate from the colorimeter will increase because the increase in detergent concentration will cause more damage to the plasma membrane of the beet root. This will cause the membrane to be less permeable, thus, allowing more betalaine pigments to be released.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work