• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Biology IA - Paper Chromotography lab report

Extracts from this document...

Introduction

Biology HL Candidate name: Ieva Drulyte Date: ___________________ Separation of nettle's photosynthetic pigments (Carotene, Phaeophytin, Xanthophyll, Chlorophyll a, Chlorophyll b) by paper chromatography CE Aim: to separate and identify photosynthetic pigments (Carotene, Phaeophytin, Xanthophyll, Chlorophyll a, Chlorophyll b) of nettle by paper chromatography putting the chromatographic paper into organic solvent - kerosene. Hypothesis: plants photosynthetic pigment chlorophyll consists of five different pigments that differ in solubility in the solvent thus each move up the chromatography paper at different rates. Also, each pigment has a different shade of color. Data collection and processing Table 1 Raw date - distances moved by the photosynthetic pigments and by solvent during 3 trials. ...read more.

Middle

The calculations made to convert raw date into Rf value: (([1] + [2] + [3]) / 3) / [4] Table 2 Calculation of the Retardation factor (Rf): Photosynthetic pigment Rf (approximate values) Rf (original value) Carotene 0.94 0.95 Phaeophytin 0.76 0.83 Xanthophyll 0.65 0.71 Chlorophyll a 0.57 0.65 Chlorophyll b 0.40 0.45 Conclusion and evaluation Five pigments of chlorophyll were separated and identified by paper chromatography: Carotene (yellow), Phaeophytin (yellow-grey), Xanthophyll (yellow - brown), Chlorophyll a (blue - green) and Chlorophyll b (green). This shows that the plant (nettle), which was examined, can pursue photosynthesis by absorbing different wavelengths, as different chlorophyll pigments absorb different wavelengths. ...read more.

Conclusion

In order to prevent from this, tweezers should be used. Thirdly, while making an extract, there are only a few moments until extract starts boiling thus enzymes could denaturate. To deal with this problem, accurate sample's observations should be done; when bubbles start collecting on the beaker's walls, immediately take it from heater. What's more, kerosene must be put into boiling tubes and the walls can not be touched with it hence when putting a chromatography paper in it, it's difficult not to make it oiled. To prevent from this problem, kerosene should be poured into boiling tube through one side of it and the paper should be put through another. ?? ?? ?? ?? 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Biology HL IA - Photosynthesis of elodea lab report

    give out a different intensity of light; therefore, it would affect the results of the experiment. The same lamp would be used throughout the experiment. Time given for photosynthesis min 10 minutes is enough time for the plant to produce enough volume of oxygen.

  2. Biology HL IA - Transpiration lab report

    Second, the photometer and branch were attached to the clamp stand and the plant was left to rest for 10 minutes. To the same clamp stand the sensors of humidity and temperature were attached in order to control the controlled variables.

  1. DNA Strawberry Extraction Lap

    The procedures to extract DNA from a strawberry are relatively similar to advanced laboratory DNA extraction procedures. Both require a mechanical disruption of cells, liberation of the DNA, and precipitation of the DNA. With the strawberries, molecules were mashed in order to break the cell wall, then, they were chemically

  2. IB biology Respiration IA

    Trial Number Time spent exercising Increment One 30 seconds (+/- .001 seconds) Time spent exercising Increment Two 60 Seconds (+/- .001 seconds) Time spent exercising Increment Three 90 seconds (+/- .001 seconds) Type of Exercise Straw Length (+/- .05 cm)

  1. Chromatography lab. Title: separation of pigments of photosynthesis using paper chromatography.

    * Uncertainty of solvent front: * Uncertainty of (d): * The average of the Rf: Points of each color appearance 1st trial 2nd trial Average The closest value in the standard Solvent front=6 Solvent front=6 Rf table D Rf D Rf Rf X1 2.5 0.42 3.5 0.58 0.50 (0.45)

  2. Experiment Colours of Light (Wavelength) absorbed by green plant

    Based on graph 2, like spinach, the graph also indicated the presence of two peaks. One is near to 425 nm and another one is around 680 nm. Another peak is formed at the bottom/through of the graph at point of wavelength of 530 nm.

  1. Biology IA - Distribution patterns of dandelions and plantains lab report (statistics)

    Plantain Dandelion Plantain 2 2 5 8 0 4 3 4 4 9 3 3 4 4 6 10 2 4 5 4 4 11 3 5 6 3 1 12 6 2 7 3 0 13 3 1 14 3 0 Conclusion and evaluation Results from the experiment proved my hypothesis that distribution of certain species (dandelion and plantain)

  2. Ecology Design IA

    the Earth, when times of sunrise and sunset should regularly be checked) *Sunrise and sunset are defined by the point at where the Sun has just touched the horizon prior to rising or setting Control of Constant Variables Variable Effect How it will be controlled Temperature of

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work