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Biology IA - Paper Chromotography lab report

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Introduction

Biology HL Candidate name: Ieva Drulyte Date: ___________________ Separation of nettle's photosynthetic pigments (Carotene, Phaeophytin, Xanthophyll, Chlorophyll a, Chlorophyll b) by paper chromatography CE Aim: to separate and identify photosynthetic pigments (Carotene, Phaeophytin, Xanthophyll, Chlorophyll a, Chlorophyll b) of nettle by paper chromatography putting the chromatographic paper into organic solvent - kerosene. Hypothesis: plants photosynthetic pigment chlorophyll consists of five different pigments that differ in solubility in the solvent thus each move up the chromatography paper at different rates. Also, each pigment has a different shade of color. Data collection and processing Table 1 Raw date - distances moved by the photosynthetic pigments and by solvent during 3 trials. ...read more.

Middle

The calculations made to convert raw date into Rf value: (([1] + [2] + [3]) / 3) / [4] Table 2 Calculation of the Retardation factor (Rf): Photosynthetic pigment Rf (approximate values) Rf (original value) Carotene 0.94 0.95 Phaeophytin 0.76 0.83 Xanthophyll 0.65 0.71 Chlorophyll a 0.57 0.65 Chlorophyll b 0.40 0.45 Conclusion and evaluation Five pigments of chlorophyll were separated and identified by paper chromatography: Carotene (yellow), Phaeophytin (yellow-grey), Xanthophyll (yellow - brown), Chlorophyll a (blue - green) and Chlorophyll b (green). This shows that the plant (nettle), which was examined, can pursue photosynthesis by absorbing different wavelengths, as different chlorophyll pigments absorb different wavelengths. ...read more.

Conclusion

In order to prevent from this, tweezers should be used. Thirdly, while making an extract, there are only a few moments until extract starts boiling thus enzymes could denaturate. To deal with this problem, accurate sample's observations should be done; when bubbles start collecting on the beaker's walls, immediately take it from heater. What's more, kerosene must be put into boiling tubes and the walls can not be touched with it hence when putting a chromatography paper in it, it's difficult not to make it oiled. To prevent from this problem, kerosene should be poured into boiling tube through one side of it and the paper should be put through another. ?? ?? ?? ?? 1 ...read more.

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