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Biology lab. Observing plant and animal cells using the light microscope

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IB BIOLOGY LABAROTORY WORK DARIA SOVA 2011 Observing plant and animal cells using the light microscope Materials: A red onion, potato, distilled water, iodine solution, methylene blue, dropping pipette, fine forcps, scalpel, microscope, slides, cover slips, tooth picks. Method: A-Red onion epidermal cell: 1.Cut the onion in four, using a scalpel, from the top to the base. Take one of the fleshy scales using the scalpel cut a square (5mm by 5mm) on the outer, convex surface, which is dark red. 2. Using fine forceps peel of the surface of one of the colored epidermal squares. Place the fragment, with the torn surface facing down, onto a slide. Add a drop of distilled water and a cover slip. 3. Under high power, carefully adjusting the diaphragm, draw a colored cell from each preparation. Add a title and label. In the microscope we can see that the onion cells have a rounded oblong shape. They zone tightly to each other. ...read more.


Mount the scrapings in a drop of iodine water on a slide. Allow the fragment of potato to soak in the iodine solution. Then place a cover slip over it. 3. Observe the preparation under the microscope. Adjust the diaphragm carefully so that the outline of the cells can be clearly seen. Draw a cell at high power and label it. Compare this slide with the banana slide. The potato tuber consists mainly of a mass of cells filled with starch and encircled by a thin corky rind. A woody fibers traverse the tubers. The cells of potato have cell wall, which supports the form of the cell, and nucleus with genetic information. There we can see much more fibers and starch. The form of the cells of potato is acute-angled. Starch in the tubers is rendered available for the nutrition of the young shoots. Banana cells are round and potato cells are acute-angled. In the potato cells there more starch than in the banana cells, the fibers are more dense, and the cells more zone tightly to each other. ...read more.


5. Draw what you see on your answer document. 6. Write 5 qualitative and quantitative observations about the cells you observed. Elodea is a genus of aquatic plants often called the waterweeds. Cells have cell wall, nucleus, cytoplasm and many chloroplasts inside of the cell near the cell wall. cells have a oblong shape. They zone tightly to each other. G- Daphnia Daphnia is a genus of small, aquatic, mostly freshwater crustaceans characterized by a single compound eye, two doubly branched antennae, and a generally translucent carapace. Daphnia are commonly called water fleas because of their jerky or jumping swimming style (although fleas are insects and thus these two groups are only very distantly related as being arthropods). H- Amoeba Amoeba is an unicellular organism. In the microscope we can see that It consist of the endoplasm, ectoplasm, cellwall, nucleus. Also it has pseudopodia. I- Oligochaeta Oligochaetes - a subclass of annelid worms of the class of Clitellata. Body length from a fraction of mm to 2,5 m. Segmentation of the body is well expressed in the inside and outside. Head, parapodii absent. Each segment of the body contains several pairs of setae. Consists of segments ...read more.

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