• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Biology Lab Report - Effects of Mouthwash on Oral Bacteria

Extracts from this document...

Introduction

BIOLOGY LAB REPORT: Investigating the effects of mouthwash on oral bacteria. Introduction Mouthwash targets bacteria in the mouth and are either antiseptic or antibacterial in nature. Antiseptics are antimicrobial substances that are applied to living tissue or skin to reduce the possibility of infection. Antiseptics are generally distinguished from antibiotics by the latter's ability to be transported through the lymphatic system to destroy bacteria within the body, and from disinfectants, which destroy microorganisms found on non-living objects. Some antiseptics are true germicides, capable of destroying microbes (bacteriocidal), whilst others are bacteriostatic and only prevent or inhibit their growth. Antibacterials are antiseptics that have the proven ability to act against bacteria. An accumulation of oral bacteria can lead to a build-up of dental plaque (the material that adheres to the teeth and consists of bacterial cells, which if are not removed through flossing and brushing, can lead to gingivitis or periodontal disease). According to the American Dental Association, regular brushing and proper flossing are enough in most, and mouthwash should only be used as a short-term solution. ...read more.

Middle

2. Without removing the lid, carefully turn the plate upside down and using a marker, divide the plate . into 4 quadrants. 3. label the quadrants. 4. Using the sterile swab, swab the inside of your cheek. 5. Open the petri dish and gently (so as not to destroy the agar) wipe the swab across the entire plate. 6. When finished swabbing the plate, quickly replace the lid. 7. Obtain three samples of mouthwash in small beakers. 8. Place a filter paper disc in each solution to soak for about lA s. 9. Carefully remove the lid from your agar plate and using forceps, place a soaked filter paper disc in each quadrant. 10. Make a note of which mouthwash disc was placed in which quadrant. 11. Seal the edges of your agar plate with tape. (From now on, the tape and lid are not to be removed!!) 12. Place your agar plate in the incubator for 48 hours. Day 2 1. Obtain your group's agar plate from the incubator - DO NOT REMOVE TAPE 2. ...read more.

Conclusion

Water is not at all effective at killing mouth bacteria. There was no zone of inhibition surrounding the filter paper soaked in water. iv. Which mouth wash is the 'best buy'? Scope-Mint. v. Was your hypothesis correct or incorrect? Explain. My hypothesis was incorrect. The experiment showed that Mouthwash B (Listerine-Cool Mint) an average zone of inhibition of 0.36cm, the lowest average zone of inhibition on bacteria compared to the other mouthwashes. Instead, mouthwash B (Scope-Mint).It showed an average zone of inhibition of 1.4cm, the highest average zone of inhibition on bacteria compared to the other mouthwashes. vi. What are the weaknesses or limitations of the lab procedure?(at least 2) The duration of the procedure was too short for substantial results to be observed. There was uneven bacterial growth over the agar, leading to inaccurate zones of inhibition. vii. What are your suggestions for improvements to the lab procedure?(based on the weaknesses listed in vi) The duration of the procedure could be extended another day to allow for more bacterial growth for more accurate results in the experiment. Ensure that the entire petri dish is completely swabbed without gaps in between. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Biology: Lab report Germination

    4 star(s)

    0.7 1.5 n/a Day 4 1.2 1 1.3 1 2.5 n/a Day 5 2.4 2.2 2.6 2.3 4 n/a Day 6 3.8 3.7 4 3.7 5.2 n/a Day 7 4.4 4.6 4.9 4.5 6.4 n/a Day 8 5.6 5.9 5.8 5.7 6.4 n/a Data Manipulation Analysis of Graph One In

  2. Vitamin C Lab

    may have an error bar compared to the other temperatures could be Kenneth and I were unfamiliar with which point the lemon juice decolourised, and we did our first titrations at room temperature. This leads to one important aspect regarding the reliability of our data - the determination of the end of point the titration.

  1. The Effects of Salinity on Wheat Germination

    Day 5 Day 6 Day 7 Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day 14 End height 2% number 1 0.0 0.0 0.0 0.0 0.0 0.5 1.0 0.5 0.5 0.5 0.5 1.5 0.5 0.5 6.0 2% number 2 0.0 0.0 0.0 0.0 0.5 1.0 0.5

  2. Biology Industrial Melanism of Peppered Moth Lab

    Calculations of the percentages of light-coloured moths and melanic moths left on intermediate-coloured "tree bark". Trial Number Percentage of Melanic Moths (%) Percentage of Light-Coloured Moths (%) 1 50.0 50.0 2 53.3 46.7 3 50.0 50.0 4 36.7 63.3 5 53.3 46.7 Processed Table 7.

  1. Plant Tropism Lab

    - - - 110 120 H-3 0 70 30 25 -5 10 H-4 - - 30 20 80 10 H-5 0 0 30 25 20 90 A-1 - - 85 85 30 30 A-2 - - - - - - A-3 - - 60 70 30 50 A-4 - -

  2. Modelling Surface Area to Volume Ratio in Cells with Agar Cubes

    As the volume of the cubes goes up the surface area to volume ratio goes down. The larger cubes have a smaller proportion of surface area than the smaller cubes. The smallest cube has a surface area to volume ratio of 6:1 and the largest cube only has a surface

  1. Botany Lab Report

    Those characteristics help plants in extreme environment to survive. Plants living in desert ecosystem usually have brown colored stem. The top of the stem is smooth and the end of the stem is rough. It means the water is stored at the top of the stem to supply leaves.

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    The graph shows the actual volume of oxygen consumption differences of germinating and non-germinating seeds in ice water at 10�C�1�C. Processed Data Graph 2: The actual volume of oxygen consumption in each 1.0 ml pipettes in ice water. The graph shows the actual volume of oxygen consumption differences of germinating and non-germinating seeds 7�C�1�C.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work