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Catalase Lab

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Introduction

Temperature of Hydrogen Peroxide on Speed of Decomposition Reaction of Chicken Liver Problem: What effect does the temperature of hydrogen peroxide, in degrees Celsius have on the speed of the decomposing the chicken live and produce oxygen and hydrogen, measured in terms of the height of the bubbles produced in centimetres(cm)? Background Information: Hydrogen peroxide is a by product of many reactions in our body. When enzymes break down amino acids and fatty acids they make large amounts of hydrogen peroxide. High amount of hydrogen peroxide can cause chemical burns and tissue damage. Catalase is a common enzyme found in most living organisms. Catalase is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive gaseous oxygen and water molecules. Biological catalyst is usually found in high concentrations in the liver. It catalyzes the decomposition of hydrogen peroxide into oxygen and water. The optimum temperature for it is 37oC and once the temperature is above 40oC, the catalase begins to denature and eventually coagulates. Also, for every 10�C increase in temperature, there is a double in the rate of reactions of enzymes within a certain range. ...read more.

Middle

Measure out 15 1mL samples of hydrogen peroxide and put each sample into a test tube. 2. Put 5 of the test tubes of hydrogen peroxide in the hot water bath and heat to 37oC, put another 5 test tubes into the beaker full of ice and cool to 7oC and leave the remaining test tubes at room temperature. 3. Once the temperature of the hydrogen peroxide reaches 7oC, 22oC or 37oC, take the chicken liver and carefully drop it into the 3% hydrogen peroxide solution in the test tubes. 4. Measure and record the height of the bubbles, in centimetres (cm), with the 30cm ruler after every 10 starting from the top of the solution's surface to the top of the bubbles. 5. Repeat steps 4 and 5 for each of the other test tubes (7oC, 22oC and 37oC) that contain 1mL of hydrogen peroxide. Evidence: Height of Bubbles Accumulated at Different Temperatures Over a Period of 30 Seconds Sample Temperature 10 seconds 1 7oC 0.6cm 2 7oC 0.8cm 3 7oC 1.0cm 4 7oC 1.1cm 5 7oC 0.9cm 6 22oC 2.0cm 7 22oC 1.4cm 8 22oC 1.2cm 9 22oC 0.9cm 10 22oC 1.6cm 11 37oC 3.3cm 12 37oC 2.5cm 13 37oC 2.9cm 14 ...read more.

Conclusion

My hypothesis was correct, but my prediction was wrong. This is because I used inappropriate information when coming up with my prediction. I used background information on the enzyme catalase when what I was really changing was the substrate (hydrogen peroxide). Limitations/Improvements: To create a better lab design, more intervals of the temperature of the hydrogen peroxide or other substrates in reactions could be used, to form a generalization about temperature change and reactivity relate to the substrates. Also only five trials were used for each temperature of hydrogen peroxide; more trials should have been used for finding a more accurate measure for the height of the bubbles. Moreover, even though the pieces of liver were the same mass, their surface area might differ. The ones with a larger surface area would have better results because a larger surface area means more enzymes are exposed to the solution, therefore more reactions can occur at the same time. This also decreases the accuracy of the results. Some modifications could be to mash up the liver so that the surface area for each 0.5g sample would be the same. ?? ?? ?? ?? ...read more.

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