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Design Lab on Enzyme Activity

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Introduction

Biology: Design Lab work Problem: To qualitatively measure the effect of the enzyme catalase on Hydrogen peroxide decomposition, then to measure the effect of the catalysis when temperature is modified. Background: The experiment is based upon the idea that an enzyme acts to lessen a substrate reaction time by lowering the energy of activation through substrate conversion via the active site. The reaction between hydrogen peroxide and catalase is an easy reaction to follow as the oxygen that is released can be collected and measured. The reaction begins swiftly as the enzyme and substrate is mixed, bubbles of oxygen are released. As the reaction continues, however, the rate at which oxygen is released gradually slows down. ...read more.

Middle

Apparatus: A manometer Manometer fluid 1 boiling tube 10 ml HâSOâ Tongs Test tube rack A rules Rubber tubing A marker pen A clamp A stop clock Two, 5ml syringe A rubber bung Method: Clamp a boiling tube to a stand and carefully insert the manometer (with fluid into a rubber bung) Using a syringe place 4cm3 of hydrogen peroxide solution into the boiling tube. Using another syringe place 0.5cm3 of catalase into the boiling tube Place the bung back into the boiling tube as quickly as possible. Start the stopwatch and take the initial reading on the manometer and then take it again after 30 seconds.( final volume) Use your final volume as your new initial volume and take the reading again after 60 seconds. ...read more.

Conclusion

Use another syringe to measure 0.5cm3 of catalase into another test tube. Place both tubes in the water bath for 30 seconds. Add the catalase to the hydrogen peroxide and place a rubber bung with a manometer inserted as quickly as possible Immediately start a stop clock and take the initial manometer reading. Measure the volume of the liquid in the manometer after 30 seconds (final volume). Repeat using the same volumes of hydrogen peroxide and catalase but place in water baths heated to 40 degrees Celsius, repeat at 50 degrees Celsius and 60 degrees Celsius. Each time measure the final volume of the liquid in the manometer after 30 seconds. Record your results in a clear table. Plot a graph of temperature against the volume of oxygen. Results: Tube1(300C) Tube2(400C) Tube3(500C) Tube4(600C) Tube5(700C) Tube5(800C) Initial volume Final volume ...read more.

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