• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Design Lab on Enzyme Activity

Extracts from this document...

Introduction

Biology: Design Lab work Problem: To qualitatively measure the effect of the enzyme catalase on Hydrogen peroxide decomposition, then to measure the effect of the catalysis when temperature is modified. Background: The experiment is based upon the idea that an enzyme acts to lessen a substrate reaction time by lowering the energy of activation through substrate conversion via the active site. The reaction between hydrogen peroxide and catalase is an easy reaction to follow as the oxygen that is released can be collected and measured. The reaction begins swiftly as the enzyme and substrate is mixed, bubbles of oxygen are released. As the reaction continues, however, the rate at which oxygen is released gradually slows down. ...read more.

Middle

Apparatus: A manometer Manometer fluid 1 boiling tube 10 ml HâSOâ Tongs Test tube rack A rules Rubber tubing A marker pen A clamp A stop clock Two, 5ml syringe A rubber bung Method: Clamp a boiling tube to a stand and carefully insert the manometer (with fluid into a rubber bung) Using a syringe place 4cm3 of hydrogen peroxide solution into the boiling tube. Using another syringe place 0.5cm3 of catalase into the boiling tube Place the bung back into the boiling tube as quickly as possible. Start the stopwatch and take the initial reading on the manometer and then take it again after 30 seconds.( final volume) Use your final volume as your new initial volume and take the reading again after 60 seconds. ...read more.

Conclusion

Use another syringe to measure 0.5cm3 of catalase into another test tube. Place both tubes in the water bath for 30 seconds. Add the catalase to the hydrogen peroxide and place a rubber bung with a manometer inserted as quickly as possible Immediately start a stop clock and take the initial manometer reading. Measure the volume of the liquid in the manometer after 30 seconds (final volume). Repeat using the same volumes of hydrogen peroxide and catalase but place in water baths heated to 40 degrees Celsius, repeat at 50 degrees Celsius and 60 degrees Celsius. Each time measure the final volume of the liquid in the manometer after 30 seconds. Record your results in a clear table. Plot a graph of temperature against the volume of oxygen. Results: Tube1(300C) Tube2(400C) Tube3(500C) Tube4(600C) Tube5(700C) Tube5(800C) Initial volume Final volume ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Browning Enzyme

    And so the test tubes were taken out at a time ranging from 17 -17.50 seconds. Some test tubes were in the water baths for slightly longer and so this would affect the results I received as the results would be larger than expected.

  2. Vitamin C Lab

    or 0.16 � 2.93% = 0.004688 or 4.69 � 10-3. The final answer would then be 0.16% � 4.69 � 10-3. Results Data Table The Vitamin C concentration uses the mean titre of each temperature. Temperature (�C) Vitamin C Concentration (%)

  1. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    test tubes in the water bath that has its temperature set to 30�?. They will be left heated for 5 minutes to ensure that they reach the temperature before the experiment continues. After 5 minutes, one at a time, one of the cut up pieces of chicken liver will be

  2. How pH effects enzyme Catalase in potato cells

    My method was easy to follow, the data table was simple and effective, especially the "notes" came in useful when processing the data, and for this evaluation. To recognise anomalous data during the experimentation, allowed me to be able to repeat the tests and gain satisfactory results, this is beneficial

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    hydrogen peroxide, as the quantity and speed of gas produced is dependant on the rate of reaction. The marked points remained the same distance apart for each reading for different enzyme concentrations so that they could be accurately compared and the trend observed.

  2. The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in ...

    Sample Size Mean Relative Rate of Enzyme activity of catalase (/s) Range of Values (/s) Standard Deviation (/s) t-value Degrees of Freedom Critical Value for p=0.05 0 5 0.28 0.23-0.34 0.044 0.501 8 2.308 20 5 0.29 0.24-0.32 0.033 20 5 0.29 0.24-0.32 0.033 2.08 8 2.308 40 5 0.24

  1. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    0.66 0.57 Raw Data Table 7: The volume of the water level at the reading of the 1.0 ml pipettes of the respirometres for 30 minutes in room temperature. The pipettes each contain, germinating seeds, non-germinating seeds and bead. Temperature (�C�1�C)

  2. Ecology Design IA

    - Counting the number of organisms attached to the rocky shore - Number of organisms measured in discrete values (#) (Data is not needed for a full 24 hours as amount of sunlight generally doesn't change after the Sun has set, unless conducting experiment at one of the poles of

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work