• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Digestion Lab. The specific purpose of this lab (activity 4) was to investigate fat digestion by pancreatic lipase and the action of bile, and to recognize whether fat digestion is ongoing or completed (2).

Extracts from this document...

Introduction

May 13, 2009 Digestive System Activity-4: Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile Objective: The human digestive system is a complex combination of mechanical and chemical processes of digestion. It consists primarily of the gastrointestinal tract, a series of organs and glands that help break down food into smaller molecules for the body to absorb and use for energy. There are six essential activities in the digestion process: ingestion, propulsion, mechanical digestion, chemical digestion, absorption, and defecation. During chemical digestion, enzymes (large protein molecules) and digestive enzymes (hydrolytic) take actions in the catabolic breakdown of food, generally begin in the mouth and conclude within the small intestine. Because digestive enzymes are highly specific and require precise environment conditions for enzyme to function optimally, their hydrolytic activity can also be studied in a test tube. The specific purpose of this lab (activity 4) was to investigate fat digestion by pancreatic lipase and the action of bile, and to recognize whether fat digestion is ongoing or completed (2). ...read more.

Middle

1 2 3 4 5 6 Additives Lipase Vegetable oil Bile salts pH 7.0 buffer Lipase Vegetable oil D.I. water pH 7.0 buffer Lipase D.I. water Bile salts pH 9.0 buffer D.I. water Vegetable oil Bile salts pH 7.0 buffer Lipase Vegetable oil Bile salts pH 2.0 buffer Lipase Vegetable oil Bile salts pH 9.0 buffer Incubation condition 37�C 60 min. 37�C 60 min. 37�C 60 min. 37�C 60 min. 37�C 60 min. 37�C 60 min. pH 6.21 6.72 9.0 7.0 2.0 8.97 The results showed that after 60 minutes of incubation, the pH for test tube 1 decreased from 7.0 to 6.21, the pH for test tube 2 (no bile salts) decreased from 7.0 to 6.72, there were no changes in pH for test tubes 3 (no vegetable oil), 4 (no lipase), and 5, and a slight decreased in pH for test tube 6. Discussion: It was the purpose of this lab to examine fat digestion by pancreatic lipase and the action of bile. ...read more.

Conclusion

Both test tubes have most substances similar, only that test tube 2 has lipase but not bile salts and test tube 4 has bile salts but not lipase. As a result, the pH in test tube 4 does not change, but the pH in test tube 2 decrease, indicating that fat digestion has occurred. Such incidents occur because the optimum pH for lipase activity is pH 7.0. Fat digestion is essential because fat is the most concentrated source of food energy. When you ingest fewer calories than your body needs, your body turns to fat for energy. However, having free fatty acids is not bad because they are necessary to absorb vitamins and a major energy fuel of liver cells and skeletal muscle, which is important for body functioning. Reference: 1. Marieb, E., & Hoehn, K. (2007). Human Anatomy & Physiology (7th ed.), p.884-947. San Francisco: Pearson Education Inc. 2. Zao P., Stabler T., Smith L., Gibson M., Zanetti N., and Lokuta A. (2006). PhysioEx 6.0 for A&P:Laboratory Simulations in Physiology. San Francisco: Pearson Education, Inc. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Design Lab

    A, B and C Procedures: * Measure the temperature of the location in which the experiment is taking place using the thermometer * Have Subject A sit down for 3 minutes without doing anything, reading anything, or listening to music, and then measure their heart rate (how many beats per minute)

  2. Biology Lab Report-Osmosis

    A solution which brings about such changes is known as hyper-osmotic solution. Each cell becomes flaccid and the chip as a whole shows the net result of these changes. In some cases, the concentration of solution outside a cell is the same as the concentration of solution inside the cell.

  1. Vitamin C Lab

    Controlled - amount of lemon juice used (2cm3) Method Equipment - one 100cm3 and three 500cm3 beakers, labels, a fresh lemon, a knife, 10cm3 pipette, burette, retort stand, safety mat, conical flasks, test tubes, test tube rack, water baths at 50�C and 80�C, thermometer, DCPIP (1% aqueous solution), deionised water, ice, funnel, pipette bulb 1.

  2. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    can reject our null hypothesis and prove there is a significant difference in both findings. This will also be further explained in my analysis section. Conclusion: In my prediction before I began my experiment, I predicted that Catalase had an optimum pH 7.5 and so the reaction would work best

  1. IB Genetic Lab

    of females, and that there is a clear association between the two factors2. This error can be improved in the future by increasing the sample size. This could be done by surveying more females to ensure that a solid conclusion can be drawn from numerous amounts of recorded values, and to increase the reliability of the experiment.

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    The differences are calculated by subtracting final value from the initial value. The actual differences are calculated by subtracting the differences in control from the differences in germinating/non-germinating seeds. Temperature (�C�1�C) Time (min) Differences in Control (Beads) ml�0.01ml Differences in Germinating Seeds ml�0.01ml Actual differences in Germinating Seeds ml�0.01ml Differences

  1. Plant Tropism Lab

    (Day 5) March 10: Pencil Pod # 3 sprouts, Kentucky Wonder # 2 grows to 10 cm, 60� (Day 6) March 11: Kentucky Wonder #2 dies, rest of beans that sprouted look close to dying. (Day 7) March 12: All Golden Wax beans explode; sprouted beans have not grown enough to measure, all look close to dying.

  2. What is the effect of temperature on the digestion of egg-white by the enzyme ...

    These results support my hypothesis of the enzyme being most efficient at 37 degrees and least efficient at 45 degrees. However I did not test the digestion rates for each degree so chances are that the optimal temperature is not 37 degrees but a temperature 35 and 40.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work