• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Effects of Temperature on Enzymes

Extracts from this document...

Introduction

Effect of temperature on enzymes DESIGN Aspect 1 - Defining the problem Investigate the effect of temperature on enzymes, by calculating the amount of gas produced in the reaction at different temperatures. Variables * Independent variable: temperature * Dependant variable: amount of gas produced * Variables not changed: amount of potato, pH, volume of H2O2, same time, type of potato and preparation of specimen. Aspect 2 - Controlling variables 1. Collect necessary equipment and materials. 2. Set up apparatus for the experiment as shown. 3. Weigh 2g of minced potato using a scale up 1/100 of a gram. 4. Put the potato in the conical flask and add 5cm3 of buffer solution at 70�C. Swirl gently to mix. Temperature must be stable while reaction is taking place. 5. Replace the bung in the flask, ensuring that it is at right seal. 6. Fill the burette with water and introduce into it the tube that comes from the flask bung. ...read more.

Middle

50�C 33.5cm3 29.5cm3 24.8cm3 23 cm3 21.8cm3 20.4cm3 60�C 35 cm3 16.5cm3 15.9cm3 15.9cm3 15.9cm3 15.9cm3 70�C 55 cm3 47.7cm3 42.3cm3 39.7cm3 39.7cm3 39.7cm3 Aspect 2 - Processing raw data Temperature 30s 60s 90s 120s 150s 30�C 1.9 cm3 4 cm3 6.1 cm3 8.5 cm3 10.7cm3 40�C 1.5 cm3 2.9 cm3 4.2 cm3 7.6 cm3 10 cm3 50�C 4 cm3 8.7 cm3 10.5cm3 11.7cm3 13.1cm3 60�C 18.5cm3 18.9cm3 18.9cm3 18.9cm3 18.9cm3 70�C 7.3 cm3 12.7cm3 15.3cm3 15.3cm3 15.3cm3 Aspect 3 - Presenting processed data Rate of reaction CONCLUSION AND EVALUATION Aspect 1 - Concluding I can conclude that the temperature affects the enzymatic activity as expected. If the temperature it is too high, the enzymes cannot work properly, however if their environment is not warm enough, their rate of reaction is lower. In this experiment, the ideal temperature at which the enzymes work the best is around 60�C, as it is shown, were the highest point in the Rate of reaction graph is at 60�C. ...read more.

Conclusion

The results however, are very good in general it was a good practical. Aspect 3 - Improving investigation There are some aspects of the experiment that could be improved to get more accurately results and to avoid anomalies such as: * Be more accurate with preparation of specimen, with the starting mass and size of it, use a machine to cute and grate it more accurately. * Making sure the scale is exact. * Potato affected by oxidation. It was not used immediately so a good improvement would be to prepare specimen and use it immediately. * Use a wider range of temperatures such as 30oC, 35 o, 40 o, 45 o, 50 o, 55 o, 60 o, 65 o, 70oC to see closer how enzymes are working. * Repeat the experiment two or three times to get more reliable results. * Make sure that temperature is always steady while reaction is happening. * Try the same experiment but with a different specimen, using carrots, apples or something else instead of potatoes. ?? ?? ?? ?? Alejandra Cossio Mart�nez 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Isotonic Point of Potatoes

    in length and mass decreased, suggesting that it was nearer to the isotonic point than any of the other solutions. The scientific reasoning is very simple for some the behaviour seen. Due to the higher concentration of certain solutions, more of the water would have flowed out of the potato

  2. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    concerning the comparison of Hydrogen Peroxide solutions of pH levels of 4 and 7 while the second one shows the results concerning the comparison of Hydrogen Peroxide solutions of pH levels of 10 and 7. Both t values are once again greater than their respective critical values and so we

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    The precision of this experiment, generally, was very limited since insufficient readings were taken. Although the range of enzyme concentrations taken was large, the difference in enzyme concentration between each reading was too great to distinguish a value between them.

  2. The Effects of Salinity on Wheat Germination

    1.0 1.0 2.0 0.0 1.0 10.0 0% number 9 0.0 0.0 0.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 2.0 0.0 1.0 11.0 0% number 10 0.0 0.0 0.0 0.0 2.0 1.0 2.0 1.0 1.0 1.0 1.0 1.0 1.0 0.0 11.0 Day 1 Day 2 Day 3 Day 4

  1. Biology- Extended essay. For this research, I investigated the effects of DDT and ...

    Through an analysis of these three investigations I will determine the change in the opercular movement of Cirrhinus Cirrhosus. 1. Taking 5 glass beakers arrange them in a line and marked them as: A, B, C, D and E. 2. Fill each beaker with 200 ml of filtered water. 3.

  2. ProblemWhat kind of roles the factors of pH levels and temperature have on various ...

    * pH, as the pH is lowered, an enzyme will tend to gain H+ ions and eventually enough side chains will be affected so that the enzyme's shape is disrupted. Likewise, as the pH is raised, the enzyme will lose H+ ions and eventually lose its active shape.

  1. Environmental Mangement - Upland Burning in Ireland and the Effects of the Heritage Bill ...

    (birdwatchireland, 2017). The current Wildlife Amendment Act, protected against the destructive and intrusive nature of burning and hedge cutting (See Fig 7 and App. Fig 12). Although often under-enforced, the Act created a framework for the protection of breeding birds. The new legislation will weaken the current laws set in place to protect breeding birds and other biodiversity.

  2. Comparing the Sexy Sons Hypothesis and the Pathogen Avoidance Models Effects on Sexual Selection

    Though it is ?better? to remain in that single mate relationship for the female because it allows her genes to be passed down instead of another member of the same species, the trait of infidelity may actually signal vigor and health in males and thus, the female will still be attracted.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work