• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Effects of Temperature on Enzymes

Extracts from this document...

Introduction

Effect of temperature on enzymes DESIGN Aspect 1 - Defining the problem Investigate the effect of temperature on enzymes, by calculating the amount of gas produced in the reaction at different temperatures. Variables * Independent variable: temperature * Dependant variable: amount of gas produced * Variables not changed: amount of potato, pH, volume of H2O2, same time, type of potato and preparation of specimen. Aspect 2 - Controlling variables 1. Collect necessary equipment and materials. 2. Set up apparatus for the experiment as shown. 3. Weigh 2g of minced potato using a scale up 1/100 of a gram. 4. Put the potato in the conical flask and add 5cm3 of buffer solution at 70�C. Swirl gently to mix. Temperature must be stable while reaction is taking place. 5. Replace the bung in the flask, ensuring that it is at right seal. 6. Fill the burette with water and introduce into it the tube that comes from the flask bung. ...read more.

Middle

50�C 33.5cm3 29.5cm3 24.8cm3 23 cm3 21.8cm3 20.4cm3 60�C 35 cm3 16.5cm3 15.9cm3 15.9cm3 15.9cm3 15.9cm3 70�C 55 cm3 47.7cm3 42.3cm3 39.7cm3 39.7cm3 39.7cm3 Aspect 2 - Processing raw data Temperature 30s 60s 90s 120s 150s 30�C 1.9 cm3 4 cm3 6.1 cm3 8.5 cm3 10.7cm3 40�C 1.5 cm3 2.9 cm3 4.2 cm3 7.6 cm3 10 cm3 50�C 4 cm3 8.7 cm3 10.5cm3 11.7cm3 13.1cm3 60�C 18.5cm3 18.9cm3 18.9cm3 18.9cm3 18.9cm3 70�C 7.3 cm3 12.7cm3 15.3cm3 15.3cm3 15.3cm3 Aspect 3 - Presenting processed data Rate of reaction CONCLUSION AND EVALUATION Aspect 1 - Concluding I can conclude that the temperature affects the enzymatic activity as expected. If the temperature it is too high, the enzymes cannot work properly, however if their environment is not warm enough, their rate of reaction is lower. In this experiment, the ideal temperature at which the enzymes work the best is around 60�C, as it is shown, were the highest point in the Rate of reaction graph is at 60�C. ...read more.

Conclusion

The results however, are very good in general it was a good practical. Aspect 3 - Improving investigation There are some aspects of the experiment that could be improved to get more accurately results and to avoid anomalies such as: * Be more accurate with preparation of specimen, with the starting mass and size of it, use a machine to cute and grate it more accurately. * Making sure the scale is exact. * Potato affected by oxidation. It was not used immediately so a good improvement would be to prepare specimen and use it immediately. * Use a wider range of temperatures such as 30oC, 35 o, 40 o, 45 o, 50 o, 55 o, 60 o, 65 o, 70oC to see closer how enzymes are working. * Repeat the experiment two or three times to get more reliable results. * Make sure that temperature is always steady while reaction is happening. * Try the same experiment but with a different specimen, using carrots, apples or something else instead of potatoes. ?? ?? ?? ?? Alejandra Cossio Mart�nez 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Isotonic Point of Potatoes

    This speculation is further strengthened by the fact that the parallax error can always occur while using analogue measuring tools, such as the ruler we used. This may have caused inaccurate readings to be taken. Also, many inaccurate readings for the change in mass may have taken place due to

  2. The Effects of Salinity on Wheat Germination

    5% number 2 0.0 0.0 0.0 0.0 0.0 0.0 0.5 0.0 0.5 0.0 0.0 1.0 0.5 0.0 2.5 5% number 3 0.0 0.0 0.0 0.0 0.0 0.0 0.5 0.0 0.0 0.5 0.5 0.0 0.5 0.0 2.0 5% number 4 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.5

  1. Should Animals have the same rights as Humans? Both animals and humans exhibit behaviours ...

    These may include shells, leaves, flowers, feathers, stones, berries, and even items of litter. The decorations take hours and may involve hundreds of items. Sometimes male birds may 'steal' items from neighbouring bowers. Studies have shown that females watch the bowers being constructed, visit and revisit many bowers and even

  2. Biology- Extended essay. For this research, I investigated the effects of DDT and ...

    SELECTING VARIABLES: - INDEPENDENT VARIABLES: I. The amount of the DDT solution added to the beakers will remain independent as we change the amount added in order to change the concentration. (1ppm, 2ppm, 4ppm, 6ppm) II. The amount of the Azo-Dye solution added to the beakers will also remain independent

  1. Environmental Mangement - Upland Burning in Ireland and the Effects of the Heritage Bill ...

    during the month of March and prepare to lay their eggs (Working Notes, 2013). Upland fires during this period could potentially eradicate future breeding sites and wipe out there populations entirely. Wild Fires Vs Prescribed Fires Wildfire refers to any uncontrolled or unplanned wildland fire that, regardless of its ignition source, requires a response (e.g.

  2. Comparing the Sexy Sons Hypothesis and the Pathogen Avoidance Models Effects on Sexual Selection

    2003). One thing that a group of evolutionary biologists discovered was that the availability of partners plays a large role in this discrepancy (Schacht, Ryan, and Adrian V. Bell 2016).

  1. Biology: Enzymes in the Dairy Industry

    Protease is another notable enzyme. Cow milk contains a number of different whey proteins such as lactoglobulin and lactalbumin. The denaturing of these whey proteins, using proteases as catalysts, results in a creamier yogurt product. The denaturing of whey proteins is also essential for cheese production.

  2. Finding the isotonic point for potato tissue

    It is also a random error because the potato sticks can be O.5 mm shorter or longer than 6 cm. Longer potato sticks would have a bigger surface area and gain more water. Shorter potato sticks would haver a smaller surface area and gain less water.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work