• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Effects of Temperature on Enzymes

Extracts from this document...

Introduction

Effect of temperature on enzymes DESIGN Aspect 1 - Defining the problem Investigate the effect of temperature on enzymes, by calculating the amount of gas produced in the reaction at different temperatures. Variables * Independent variable: temperature * Dependant variable: amount of gas produced * Variables not changed: amount of potato, pH, volume of H2O2, same time, type of potato and preparation of specimen. Aspect 2 - Controlling variables 1. Collect necessary equipment and materials. 2. Set up apparatus for the experiment as shown. 3. Weigh 2g of minced potato using a scale up 1/100 of a gram. 4. Put the potato in the conical flask and add 5cm3 of buffer solution at 70�C. Swirl gently to mix. Temperature must be stable while reaction is taking place. 5. Replace the bung in the flask, ensuring that it is at right seal. 6. Fill the burette with water and introduce into it the tube that comes from the flask bung. ...read more.

Middle

50�C 33.5cm3 29.5cm3 24.8cm3 23 cm3 21.8cm3 20.4cm3 60�C 35 cm3 16.5cm3 15.9cm3 15.9cm3 15.9cm3 15.9cm3 70�C 55 cm3 47.7cm3 42.3cm3 39.7cm3 39.7cm3 39.7cm3 Aspect 2 - Processing raw data Temperature 30s 60s 90s 120s 150s 30�C 1.9 cm3 4 cm3 6.1 cm3 8.5 cm3 10.7cm3 40�C 1.5 cm3 2.9 cm3 4.2 cm3 7.6 cm3 10 cm3 50�C 4 cm3 8.7 cm3 10.5cm3 11.7cm3 13.1cm3 60�C 18.5cm3 18.9cm3 18.9cm3 18.9cm3 18.9cm3 70�C 7.3 cm3 12.7cm3 15.3cm3 15.3cm3 15.3cm3 Aspect 3 - Presenting processed data Rate of reaction CONCLUSION AND EVALUATION Aspect 1 - Concluding I can conclude that the temperature affects the enzymatic activity as expected. If the temperature it is too high, the enzymes cannot work properly, however if their environment is not warm enough, their rate of reaction is lower. In this experiment, the ideal temperature at which the enzymes work the best is around 60�C, as it is shown, were the highest point in the Rate of reaction graph is at 60�C. ...read more.

Conclusion

The results however, are very good in general it was a good practical. Aspect 3 - Improving investigation There are some aspects of the experiment that could be improved to get more accurately results and to avoid anomalies such as: * Be more accurate with preparation of specimen, with the starting mass and size of it, use a machine to cute and grate it more accurately. * Making sure the scale is exact. * Potato affected by oxidation. It was not used immediately so a good improvement would be to prepare specimen and use it immediately. * Use a wider range of temperatures such as 30oC, 35 o, 40 o, 45 o, 50 o, 55 o, 60 o, 65 o, 70oC to see closer how enzymes are working. * Repeat the experiment two or three times to get more reliable results. * Make sure that temperature is always steady while reaction is happening. * Try the same experiment but with a different specimen, using carrots, apples or something else instead of potatoes. ?? ?? ?? ?? Alejandra Cossio Mart�nez 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. The Effects of Salinity on Wheat Germination

    3 0.0 0.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 2.0 1.0 0.0 12.0 0% number 4 0.0 0.0 0.0 1.0 1.0 1.5 1.0 1.0 1.0 1.0 0.0 1.0 1.0 0.0 9.5 0% number 5 0.0 0.0 0.0 1.0 1.0 1.0 2.0 1.0 2.0 1.0 1.0 2.0 1.0

  2. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    can reject my null hypothesis and that there is a significant difference between the oxygen levels produced between these two pH levels. The uncertainty here is similar to the one mentioned above. Significant differences in the oxygen levels given off between Hydrogen Peroxide pH 1 and pH 7 and compared

  1. Isotonic Point of Potatoes

    Mass Before (g) (+/- 0.01 g) Mass After(g) (+/- 0.01 g) Length Before (+/- 0.05 cm) (cm) Length After (+/- 0.05 cm) (cm) Sample 1 1.78 1.76 3.00 2.95 Sample 2 1.75 1.74 3.00 3.00 Sample 3 1.67 1.70 3.00 3.00 Sample 4 1.82 1.85 3.00 3.00 Sample 5 1.75

  2. Biology- Extended essay. For this research, I investigated the effects of DDT and ...

    Continue the above procedure with different concentrations of DDT as 2ppm, 4ppm and 6ppm respectively. 19. The same experiment now has to be laid out to carry out the second and third investigation regarding the Azo Dye and the mixture between DDT and the Azo Dye.

  1. Should Animals have the same rights as Humans? Both animals and humans exhibit behaviours ...

    www.pbs.org/lifeofbirds/brain/index.html Humans routinely use the trial and error process to solve problems, learn new knowledge or fix things. The learning occurs when changes are made which result in success (the reward) and mistakes (punishments) do not get repeated. With behavioural imprinting a new born animal is able to distinguish its own parents from other animals of the same species.

  2. ProblemWhat kind of roles the factors of pH levels and temperature have on various ...

    * In an enzyme-catalyzed reaction, the substance to be acted upon, the substrate, binds reversibly to the active site of the enzyme. * The active site is the portion of the enzyme that interacts with the substrate, so that any substance that blocks or changes the shape of the active site affects activity of that enzyme.

  1. Activated sludge wastewater treatment system and method

    of previously known aeration tank waste treatment systems that it is not necessary to continuously divert a large portion of the solids produced by the solid/liquid separator. The concentration of activated sludge solids in the digester tank is quite high, generally being on the order of 5,000 to 100,000 milligrams per liter, with 40,000 milligrams per liter being typical.

  2. Biology independent investigation, Ecology

    1.1.2-Control Used for Comparison - Distilled water as it is pure and will not have any fluorine present 1.1.3- Apparatus and Materials (Table 2) Materials- Materials- Quality sodium fluoride NaF for stock solution 5g stock solution (5mg in 500mls) (100ppm) 500ml vermiculite 250g distilled water 1L Apparatus Quantity Pipette (5mls)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work