• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzyme Investigation. How does the concentration of hydrogen peroxide affect the amount of oxygen produced by the enzyme catalase?

Extracts from this document...

Introduction

Enzyme Investigation Introduction An enzyme is a biological catalyst that alters the rate of a reaction without being changed itself. Enzymes are three dimensional shape proteins that has a very specific active site. "In enzymatic reactions, substrates are converted into different molecules also known as products. Since enzymes are very selective for their substrates, each enzyme can only convert one kind of substrate into one kind of product molecule.1 Research Question How does the concentration of hydrogen peroxide affect the amount of oxygen produced by the enzyme catalase? Hypothesis If catalase is added to the varying levels of dilution of hydrogen peroxide samples, the solution with the highest substrate concentration will yield the most activity, thus producing more oxygen gas. This assumption is based on scientific theory, which states that higher substrate concentrations increase the ability of enzymes to catalyze reactions. 2 As substrate concentration increases, the amount of substrate per unit volume increases. So it is more likely than an en enzyme molecule collides with a substrate molecule, as more successful collisions are more likely to occur. ...read more.

Middle

Size/SA of potato Each potato piece would be obtained using a core borer to ensure the diameter of each piece is constant. Each piece will also be 2cm long by using a ruler to measure each one. Time For each experiment, the potato pieces would be left in the hydrogen peroxide to react for 5 minutes after which the volume of oxygen produced would be recorded. A timer would be used to time accurately. Volume of solution (cm3) 20 cm3 of solution will be used for each experiment. The concentration however, may vary with the amount of hydrogen peroxide and water as long as the volume totals 20 cm3. Measuring cylinders will be used for accurate measurement. Type of substrate & enzyme The type of substrate and enzyme has to be the same for the whole experiment to work. The substrate is the hydrogen peroxide and the enzyme is catalase, which is obtained from potatoes. Equipment Equipment such as measuring cylinders, conical flasks and gas syringes will need to be the same. ...read more.

Conclusion

The table below shows the amount of hydrogen peroxide and distilled water need to prepare different glucose concentrations. Amount of glucose (cm3) Amount of distilled water (cm3) Concentration (%) Total volume (cm3) 4 16 20 20 8 12 40 20 12 8 60 20 16 4 80 20 20 0 100 20 6. 7. Add one sample to the conical flask with the potato 8. Quickly secure the conical flask with the delivery tube and bung to secure the that the maximum of oxygen can be produced and into the syringe 9. Time (10mins?) 10. Measure amount of oxygen obtained from gas syringe 11. Repeat with the other samples of hydrogen peroxide 12. Repeat experiment 4 more times 1Smith AL (Ed) (1997). Oxford dictionary of biochemistry and molecular biology. Oxford [Oxfordshire]: Oxford University Press 2 Ward, William, and Alan Damon. "Chaper 3- Chemistry Of Life." Pearson Baccalaureate: Higher Level (plus Standard Level Options) : Biology Developed Specifically for the Ib Diploma. Harlow, [England: Pearson Education, 2007. 68-69. Print. ?? ?? ?? ?? Claudia Cheng ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Peer reviewed

    Enzyme Coursework. Investigation to find the effect of substrate concentration on the rate ...

    5 star(s)

    and add 10cm� hydrogen peroxide solution into the appropriate test tubes. Preparing the pieces of paper Fill three petri dishes with the catalase enzyme solution and soak 5 pieces of paper into each. Investigating the effect of substrate concentration on reaction time Using tweezers, take a piece of paper from

  2. Browning Enzyme

    Borer - Ruler - Knife - Pipette - Tile Retrieving the Juice - Filter Paper - Another set of Twenty-Five test tubes - Crucible set ( with a pestol) - Twenty- Five cuvettes - Three Stands - Pipette - Measuring Cylinder - Colorimetre - Method 1) Take one apple 2)

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    3 Describe the shape of the graph. At low concentrations, the graph will show an increasing rate of reaction as concentration increases, levelling off at higher concentrations. 4 Explain the shape of the graph in relevant biological terms. The shape is explained by the concentration of substrate directly affecting the rate of reaction until another limiting factor becomes more important.

  2. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    the same pH levels for each of the Hydrogen Peroxide solutions with the altered pH, like for example the pH level for the Hydrogen Peroxide that has been altered to pH 1 might not be exactly the same in experiment 1, 2 or 3 and so therefore this is also

  1. internal assessment enzyme

    At first, the colour of the mixture added to the drops of iodine is dark blue, but soon afterwards it gradually into the original colour of the iodine drops. 3. There is a constant blue-black colour change for the control experiment.

  2. How pH effects enzyme Catalase in potato cells

    Measure 10ml of pH3 and add to test tube. 5. Quickly inject 10ml of hydrogen peroxide 6. Plug the rubber cork attached to the U-shaped tube on to the test tube 7. Let the catalase reactions begin for 15 seconds.

  1. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    at the reading of the 1.0 ml pipettes of the respirometres for 30 minutes in ice water. The pipettes each contain, germinating seeds, non-germinating seeds and bead. Room Temperature: Temperature (�C�1�C) Time (min) Control (Beads) ml�0.01ml Germinating Seeds ml�0.01ml Non-germinating Seeds ml�0.01ml 21 0 0.92 0.82 0.88 5 0.91 0.80

  2. How does cooking affect the amount of vitamin C in lemon juice?

    A longer cooking time will cause more vitamin C molecular structures to be destroyed, which result to a low vitamin C concentration. From the bar chart plotted, it can be observed that 1 hour boiled lemon juice contains the highest concentration of vitamin C, followed by fresh lemon juice and 10 minutes boiled lemon juice.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work