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Enzyme Lab. How does altering pH affect the reaction rate of catalase on hydrogen peroxide?

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Introduction

Question: How does altering pH affect the reaction rate of catalase on hydrogen peroxide? Independent: the acidity or alkalinity of the hydrogen peroxide Dependent: The reaction rate of catalase on the hydrogen peroxide Controlled Variables: The concentration of catalase (i.e. the mass of the liver used in each solution of hydrogen peroxide) Temperature The concentration of the hydrogen peroxide (the volume of hydrogen peroxide in the beaker) ...read more.

Middle

Procedure: 1. Using the scalpel and tweezers cut 6 pieces of liver, each having a mass of 2 g 2. Place 6 100 mL beakers adjacent to each other; fill each beaker with 50 mL of hydrogen peroxide solution 3. Label the beakers controlled, pH 3, pH 5, pH 7, pH9 and pH 11 4. Using the pH probe, measure the pH of the beaker labeled Controlled and record the value in the data table below 5. ...read more.

Conclusion

6. Using a thermometer, measure the temperature of each solution to assure that the temperature is constant. 7. Place the 2 g liver into the beaker marked controlled. Start the timer when bubbles start to appear. Stop the timer as the solution stops bubbling. Record the time in the observation chart below. 8. Repeat step 7 for the other 5 beakers. 9. Graph the observations onto the graph below. Effect of pH on Reaction Rate pH Rate of reaction (s) Controlled: 3 5 7 9 11 The Rate of Reaction of Catalase under Various pH levels ...read more.

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