• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Enzyme Lab. pothesis: If liver and potato tissue are tested for the amount of catalase and peroxidase, then it will show that liver tissue has more catalase than potato tissue has peroxidase.

Extracts from this document...

Introduction

Enzyme Activity in Liver and Potato Tissue I. Design: A. Problem: Which tissue (liver or potato) has more catalase/peroxidase? B. Hypothesis: If liver and potato tissue are tested for the amount of catalase and peroxidase, then it will show that liver tissue has more catalase than potato tissue has peroxidase. C. Variables: 1. Independent Variable: type of tissue 2. Dependent Variable: gas pressure 3. Controlled Variables: amount of tissue, amount and concentration of hydrogen peroxide, time D. Method (Procedure): 1. Materials: Logger Pro Vernier Gas Pressure Sensor pipettes 3% H2O2 1-hole rubber stopper test tube liver tissue potato tissue test tube rack goggles graduated cylinder scale Vernier Computer Interface Enzyme Suspension 2. Procedure: 1. Obtained and wore goggles. 2. Connected the Gas Pressure Sensor to the computer interface. 3. Connected the plastic tubing to the valve on the Gas Pressure Sensor. 4. Placed the test tube in a test tube rack. 5. Measured 1 mL of 3% H2O2 and added it to the test tube. 6. Connected the plastic tubing to a rubber stopper. ...read more.

Middle

I will keep the amount of tissue (liver and potato) constant throughout each trial by measuring each sample on a scale. 4. Method: Sufficient and Relevant Data Collection: This method will enable an adequate data collection to address the research question. First, the controlled variables will allow for accurate data with minimal errors. Additionally, the method requires the use of technology, which will ensure accurate data, with less chance of human error. Lastly, this method allows for multiple trials to be run which helps qualify the quantitative data and ensure accuracy. II. Data Collection and Processing A. Raw Data: Change in Gas Pressure of Potato and Liver Tissue Trial # Type of Issue Initial Pressure (kPa/sec) Final Pressure (kPa/sec) Rate of Change in Gas Pressure (kPa/sec) Control N/A 99.92 99.92 N/A 1 Potato 100.82 104.12 0.06 1 Liver 101.27 118.75 0.19 2 Potato 100.31 101.62 0.02 2 Liver 100.22 116.02 0.24 3 Potato 100.49 104.40 0.06 3 Liver 100.53 116.99 0.19 B. Data Processing: 1. Overview: The data in the data chart will be corresponded to in a graph that will display the different rates of change for each tissue for each trial. ...read more.

Conclusion

Nevertheless, the texture of the liver tissue prevented me from cutting it into smaller pieces. Additionally, the size of the potato pieces that were created when I cut up the 2 grams was an added variable that I did not account for. Even though I cut the potato for each trial, the size of the resulting pieces varied between trials, changing the surface area which could have an effect on the accuracy of the data. C. Improving the Investigation: The accuracy of the data is limited due to the texture and shape of the liver and potato tissue. The liver was randomly shaped while the potato was chopped up into smaller pieces. This changed the quantity of surface area available for the reaction with the H2O2. This inaccuracy could be eliminated by blending the tissues to achieve the same consistency. This would ensure that the surface areas of each tissue of each trial will be constant. It will make the surface area of the tissues a controlled variable, guaranteeing more accurate data. Additionally, more trials could be run with varying amounts of each tissue to give a greater variety of data to further support the conclusions and hypothesis. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Browning Enzyme

    Perhaps, next time I can ask other people to help me take out the five samples at the same time rather than me taking them out one by one and hence increasing the time at which some samples remained in the water baths, this would improve the accuracy of my results.

  2. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    This means that the results of the experiment are presented in a clear and orderly fashion that allows patterns in the results to become more obvious. The rate of reaction was calculated by dividing 1000 by the time taken for the quantity of gas to be produced from the reaction.

  1. Influence of pH on the activity of potato catalase

    Fourth 221.0 191.0 172.0 169.0 80.0 82.0 STANDARD DEVIATION: 10.6 10.3 7.8 15.7 9.8 9.9 Rounded to 1 decimal place Using the formula , the standard error was calculated: Table 9: The standard error calculated for each pH Time Trial [s] �0.5 s pH 3.0 4.0 5.0 6.0 7.0 8.0

  2. In this extended essay I am looking at the effect of different kind of ...

    Plant2 (cm) Plant3 (cm) Plant4 (cm) Plant5 (cm) Average height (cm) Classical 26.8 26.4 25.3 26.2 27.7 27.0 Rock 23.5 26.7 27.9 28.6 27.5 26.4 Traffic 28.6 23.2 25.0 26.5 22.3 25.3 No sound 26.6 24.5 29.2 21.5 24.9 25.1 Day 12: The data collected on Day 12: Name of music Played on plant Plant1 (cm)

  1. Vitamin C Lab

    / Measurements (cm3) � 100 Uncertainty Percentage (%) �0.1 21.8 0.46 17.0 0.59 23.7 0.42 18.9 0.53 Overall uncertainty (%) + 4 (uncertainty of using the pipette twice for two titrations) 6.00 Sample calculation with overall uncertainties If Vitamin C concentration in lemon juice at 10�C is 0.16%, the uncertainty is 2.93% (calculated above)

  2. The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in ...

    rates of enzyme activity for the target 20�C catalase temperature data set = number of relative rates of enzyme activity for the target 40�C catalase temperature data set Degrees of Freedom: For target 20�C Enzyme Catalase Temperature Data Set and the target 40�C Enzyme Catalase Temperature Data set Where df

  1. The effect of pvc piping on the breathing/heart rates of male year 12 students

    beats per minutes and breaths per minute were averaged and placed into a table and scattergram graph. This allowed the compassion of individual lengths (or control) in relation to other points. It also allowed direct comparison between the two graphs (each depended variable)

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    at the reading of the 1.0 ml pipettes of the respirometres for 30 minutes in ice water. The pipettes each contain, germinating seeds, non-germinating seeds and bead. Room Temperature: Temperature (�C�1�C) Time (min) Control (Beads) ml�0.01ml Germinating Seeds ml�0.01ml Non-germinating Seeds ml�0.01ml 21 0 0.92 0.82 0.88 5 0.91 0.80

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work