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Enzyme Lab. pothesis: If liver and potato tissue are tested for the amount of catalase and peroxidase, then it will show that liver tissue has more catalase than potato tissue has peroxidase.

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Introduction

Enzyme Activity in Liver and Potato Tissue I. Design: A. Problem: Which tissue (liver or potato) has more catalase/peroxidase? B. Hypothesis: If liver and potato tissue are tested for the amount of catalase and peroxidase, then it will show that liver tissue has more catalase than potato tissue has peroxidase. C. Variables: 1. Independent Variable: type of tissue 2. Dependent Variable: gas pressure 3. Controlled Variables: amount of tissue, amount and concentration of hydrogen peroxide, time D. Method (Procedure): 1. Materials: Logger Pro Vernier Gas Pressure Sensor pipettes 3% H2O2 1-hole rubber stopper test tube liver tissue potato tissue test tube rack goggles graduated cylinder scale Vernier Computer Interface Enzyme Suspension 2. Procedure: 1. Obtained and wore goggles. 2. Connected the Gas Pressure Sensor to the computer interface. 3. Connected the plastic tubing to the valve on the Gas Pressure Sensor. 4. Placed the test tube in a test tube rack. 5. Measured 1 mL of 3% H2O2 and added it to the test tube. 6. Connected the plastic tubing to a rubber stopper. ...read more.

Middle

I will keep the amount of tissue (liver and potato) constant throughout each trial by measuring each sample on a scale. 4. Method: Sufficient and Relevant Data Collection: This method will enable an adequate data collection to address the research question. First, the controlled variables will allow for accurate data with minimal errors. Additionally, the method requires the use of technology, which will ensure accurate data, with less chance of human error. Lastly, this method allows for multiple trials to be run which helps qualify the quantitative data and ensure accuracy. II. Data Collection and Processing A. Raw Data: Change in Gas Pressure of Potato and Liver Tissue Trial # Type of Issue Initial Pressure (kPa/sec) Final Pressure (kPa/sec) Rate of Change in Gas Pressure (kPa/sec) Control N/A 99.92 99.92 N/A 1 Potato 100.82 104.12 0.06 1 Liver 101.27 118.75 0.19 2 Potato 100.31 101.62 0.02 2 Liver 100.22 116.02 0.24 3 Potato 100.49 104.40 0.06 3 Liver 100.53 116.99 0.19 B. Data Processing: 1. Overview: The data in the data chart will be corresponded to in a graph that will display the different rates of change for each tissue for each trial. ...read more.

Conclusion

Nevertheless, the texture of the liver tissue prevented me from cutting it into smaller pieces. Additionally, the size of the potato pieces that were created when I cut up the 2 grams was an added variable that I did not account for. Even though I cut the potato for each trial, the size of the resulting pieces varied between trials, changing the surface area which could have an effect on the accuracy of the data. C. Improving the Investigation: The accuracy of the data is limited due to the texture and shape of the liver and potato tissue. The liver was randomly shaped while the potato was chopped up into smaller pieces. This changed the quantity of surface area available for the reaction with the H2O2. This inaccuracy could be eliminated by blending the tissues to achieve the same consistency. This would ensure that the surface areas of each tissue of each trial will be constant. It will make the surface area of the tissues a controlled variable, guaranteeing more accurate data. Additionally, more trials could be run with varying amounts of each tissue to give a greater variety of data to further support the conclusions and hypothesis. ?? ?? ?? ?? ...read more.

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