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Examine how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase.

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Introduction

The Effect of Substrate Concentration on the activity of the Enzyme Catalase Aim: Examine how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. Hypothesis: I believe that as the concentration of the substrate increases, the rate of reaction will also increase. However the reaction will stop when the solution is saturated with the substrate hydrogen peroxide. Background: Enzyme Catalase, are protein molecules found in all living cells. This type of enzyme is found in foods such as potatoe. Enzymes are natural catalyzers, and they are used to speed up reactions and are specific because each one performs one type of reaction. This experiment will show how this enzyme breakdown the hydrogen peroxide molecule by speeding up the decomposition of it into water and oxygen. Equation of this experiment: Catalase Hydrogen Peroxide---------------------->Water + Oxygen Catalase 2H2O2------------------->2H2O+O Variables * Independent variable: Amount of enzymes * Dependant variable: Is the amount of hydrogen peroxide used and the materials such as test tubes and measuring cylinders. ...read more.

Middle

Average 18 43 47 60 80% 30 60 90 120 AM 12 55 62 78 JM 12 25 32 39 VC 38 51 71 83 Nico 10 23 30 32 Ch 15 30 52 38 Average 17 37 49 54 60% 30 60 90 120 AM 14 49 60 70 JM 1 12 18 22 VC 9 12 16 20 Nico 15 28 35 40 Ch 5 25 37 42 Average 8.8 25 33 39 40% 30 60 90 120 AM 7 14 40 44 JM 4 10 22 30 VC 11 22 51 60 Nico 22 40 52 57 Ch 22 40 46 50 Average 13 25 42 48 Graph: Conclusion: Our aim was completed, as we could examine how the concentration of hydrogen peroxide affects the rate of reaction of the enzyme catalase. The rate of reaction is increased until the concentration of hydrogen peroxide is saturated at 90s. ...read more.

Conclusion

The reaction is the fastest compared to the other two sections. Section B, there still reaction between them, however it slowed down. Section C, starts at the 90 seconds. I already mentioned that at this period of time the hydrogen peroxide becomes saturated. By watching the graph, we can say that the rate of reaction takes more time to occur. The independent variable, amount of enzymes, was different to the dependant, which was the amount of hydrogen. This was done so, to see the difference between them at the end of the experiment. These differences, has shown us that the more substrate in the reaction, the more product that will be produce. To reduce errors and obtain more accurate results, we maintained the independent variable always constant. Repetition means, reduce of errors. Therefore we should have repeated the experiment three times approximateley. However, there are some errors which, need to take into account. For example, our reaction in order, to start and stop the stopwatch in the correct time. ?? ?? ?? ?? Celina Campo Biology IB ...read more.

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