• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment to find the water potential of the cytoplasm of potato cell (Solanum tuberosum linnaeus

Extracts from this document...

Introduction

To find the water potential of the cytoplasm of potato cell (Solanum tuberosum linnaeus) Observation: 1. Potato (Solanum tuberosum linnaeus) 1 strips turn into dark colour when it gets oxidized because it exposed to air too long. 2. Potato (Solanum tuberosum linnaeus) strips become turgid after putting in the 0.1 mol sucrose concentration for 30 minutes. 3. Potato (Solanum tuberosum linnaeus) strips become flaccid after putting in the 0.5 sucrose concentration for 30 minutes. Raw data: 2.5cm potato (Solanum tuberosum linnaeus) strips, cock borer number 1 and number 2 Table 1.1 Mass of potato (Solanum tuberosum linnaeus) strips before putting into sucrose solution Concentration of sucrose solution/mol dm3 Trial Mass of potato (Solanum tuberosum linnaeus) strips before adding into sucrose solution/g (0.01g) Mass of potato (Solanum tuberosum linnaeus) strips after adding into sucrose solution/g (0.01g) 0.1 1 0.53 0.58 2 0.55 0.59 3 0.58 0.62 4 0.57 0.61 5 0.57 0.61 0.2 1 0.56 0.58 2 0.58 0.61 3 0.57 0.60 4 0.63 0.65 5 0.65 0.66 0.3 1 0.56 0.55 2 0.58 0.58 3 0.57 0.57 4 0.72 0.71 5 0.75 0.74 0.4 1 0.62 0.60 2 0.66 0.64 3 0.60 0.59 4 0.55 0.54 5 0.60 0.58 0.5 1 0.61 0.56 2 0.61 0.57 3 0.70 0.66 4 0.60 0.57 5 0.60 0.56 Processing data: Concentration of sucrose solution/mol dm3 Trial Change of mass of potato (Solanum tuberosum linnaeus) ...read more.

Middle

When there is no net movement between water molecules, the water potential of solvent and the water potential of the solute are equal. The range of literature value of water potential is from 0.24mol to 0.31mol, which estimate to be -680 to -860 kPa5 because 0.24mol has water potential -680kPa and 0.30 mol has water potential -860 kPa. From my results (refer to figure 1.1), I found that concentration of sucrose is in 0.309mol 0.3mol and water potential ) would be -860kPa3 is in Isotonic which is when water potential of potato (Solanum tuberosum linnaeus) strips and water potential of sucrose solution is equal. My results are in the range of the literature value, which means my results are quite accurate. From this experiment I found that potato (Solanum tuberosum linnaeus) strips decrease in mass as well as in turgidity from concentration of 0.1 to 0.5mol. Potato (Solanum tuberosum linnaeus) strips put in 0.1 are the hardest and most turgid among others. Potato (Solanum tuberosum linnaeus) strips put in 0.5mol are the softness and most flaccid among others. Therefore, masses of potato (Solanum tuberosum linnaeus) strips in 0.1 mol of sucrose concentration gained the most in mass since sucrose solution in 0.1M is less concentrated, therefore there is low sucrose concentration and ...read more.

Conclusion

I think we can only improve above error by doing the experiment for three times or more because we cannot find five potato (Solanum tuberosum Linnaeus) strips with the same water potential for each concentration. However, we can compare the results together or find the average then see which results give a more significant difference to get a more accurate answer. Since potato (Solanum tuberosum linnaeus) strips will get oxidized if it exposes to air too long, therefore we need to prevent water evaporated. To make a fair test, we should start the experiment as soon as we can once we have pushing out 5 pieces of potatoes (Solanum tuberosum linnaeus) strips, so it can avoid water evaporated from potato (Solanum tuberosum Linnaeus) strips, make a fair test and get a more accurate answer. Another possible error may occur when we took out the potato (Solanum tuberosum Linnaeus) strips from the sucrose solution and dry them on the tissue paper because every time we use different forces and sometimes water may lose if we press too hard. To improve it, I think we can repeat the experiment for three times or more then we can find the average and compare the results to get a more accurate answer. _____________________________________________________________________ 1http://www.shgresources.com/id/symbols/vegetable/ 2 http://www.graphpad.com/quickcalcs/ttest1.cfm 3 http://dl.clackamas.edu/ch105-04/calculat.htm 4 http://www.ruf.rice.edu/~bioslabs/tools/stats/ttable.html 5 http://biology.clemson.edu/bpc/bp/Lab/110/osmosis.htm ?? ?? ?? ?? Biology IA Vivian Kok 12C ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. The effect of concentration of sucrose solution on the osmosis in potato

    (5 test tubes for each concentration of sucrose solution) * Leave the potatoes in the solution for a total of 24 hours. * Wipe the potatoes with tissue paper so there is no liquid on them and then weigh them and record the mass.

  2. Osmosis. By this investigation, I tried to find out the changes in potato ...

    The data from this investigation shows that there are different results for potato and sweet potato cylinders. Therefore, we know that water potential (and concentration of sucrose) inside the potato and sweet potato cells is different. On the other hand, in both, by increasing outside solution concentration, water potential was gradually increasing in the cell.

  1. Investigation of the affect of salinity on Osmosis within Solanum tuberosum

    As cell membranes allow water to pass through, any high concentration of solute relative to the cell can have a dramatic affect on the diffusion of water from the cell. When a cell is placed in a hypertonic solution, containing a high concentration of any substance, the water easily diffuses

  2. What is the effect of different concentration of salt solution on the rate of ...

    Cutting board 6. Ruler 7. Stopwatch 8. Paper towel Method: 1. Cut potato into strips by borer to 3.0cm long. Prepare 25 of them. 2. Prepare 5 different concentration of salt solutions with NaCl and 50ml of distilled water each in the beakers and label them as below: Table 1 - NaCl-H2O Dilutions NaCl concentration/M NaCl /g

  1. Beet Lab. Aim: To determine the effects of temperature on the permeability of ...

    value Total % Error Systematic Error 20oC 45.78 % 99 53.76% 47.76% 30oC 41.56 % 99 58.02% 52.86% 40oC 41.28 % 98 57.87% 53.14% 50oC 31.3 % 98 68.06% 63.6% 60oC 16.62 % 93 82.13% 77.83% Absorbance Temperature Average Literature value Total % Error Systematic Error 20oC 0.342 0.03 10.4%

  2. Designing an Osmotic Potential Experiment with Potatoes

    * Potato, I will use one or more potatoes. * Sugar, sugar will be used as the solute. * Spoon, to stir the solution. * Clock, to monitor the time. * Still water, to be the solvent. * Scale, to measure the mass of the potato and sucrose. * Knife, to cut the potato.

  1. An experiment to show the water potential of potato cells

    Variables The independent variable is the concentration of sucrose solution in which the potato cylinder is placed in (0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7 mol ml-3). The dependent variable is the percentage change of mass (grams) of the potato cylinders before and after the experiment and the percentage

  2. Finding the isotonic point for potato tissue

    Explanation : A : At 0.31 M, the potato sticks do not change in mass. This is because the concentration of the sucrose solution and the concentration of the solute in the cell cytoplasm is the same, they are isotonic.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work