• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How does the concentration of hydrogen peroxide affect the time (s) it takes the enzyme catalase to decompose it into water and oxygen gas?

Extracts from this document...


Biology Hl-Enzyme Design IA Elena Mastin Wood Research Question: How does the concentration of hydrogen peroxide affect the time (s) it takes the enzyme catalase to decompose it into water and oxygen gas? Variables: Dependent: 1. Time (in seconds) taken for the decomposition reaction to be complete. 1. Measured using a stopwatch Independent: 1. Molar concentration (mol/L) of hydrogen peroxide (H2O2) 1. Solutions will be increased in concentration from 1 mol/L to 1.25 mol/L, to 1.5 mol/L, to 1.75 mol/L, to 2 mol/L. Controlled: 1. Mass (in grams) ...read more.


1. Volume of hydrogen peroxide 1. A standard volume of 20mL of hydrogen peroxide will be used in each test, with ample amounts of water to change the concentration. 2. If it was measured using 20mL of the hydrogen peroxide solution, there would be less of the substance at lower concentrations, which would speed the reaction and possibly skew the results. 1. Container used 1. The same 250 mL beaker will be used for each test. 2. The surface area that is exposed will be kept constant in order to keep the area of reaction between the substrate and the enzyme constant, so as not produce inaccurate data. ...read more.


Stopwatch 4. 250mL beaker. Procedure 1. Measure 10g of pureed cow?s liver into a 250mL beaker. 2. Measure 20mL of 100% hydrogen peroxide and dilute to 1mol/L of hydrogen peroxide solution. 3. Pour the hydrogen peroxide solution into the beaker, and start timer. 4. Stop timer when the mixture ceases to bubble, signaling the end of the reaction. 5. Record time into a data table. 6. Repeat steps 1-5 with four more trials. 7. Repeat steps 1-6 with 1.25 mol/L of hydrogen peroxide solution. 8. Repeat steps 1-6 with 1.5 mol/L of hydrogen peroxide solution. 9. Repeat steps 1-6 with 1.75 mol/L of hydrogen peroxide solution. 10. Repeat steps 1-6 with 2 mol/L of hydrogen peroxide solution. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    5 Describe any technical difficulties you had with this apparatus and explain how these could be overcome. Students should be able to describe technical difficulties and suggest how these could be overcome. 6 Describe how you would extend this investigation to provide more evidence/ data to support your understanding of enzyme-controlled reactions.

  2. The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in ...

    the lower temperatures of catalase enzyme of 20�C and 40�C support the hypothesis as they are close to the 35-40�C optimal temperature. Overall the data and values support the hypothesis. Evaluation: The filter paper disks were drying up quickly after being patted with a paper towel to remove excess liquid.

  1. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    for the first two experiments, I used the same chicken liver, however, for the third experiment I used fresh chicken liver to get the Catalase enzyme and so therefore this could also be an uncertainty in that it could affect the quality or activity of the Catalase used.

  2. Aim: To investigate whether the temperature effects the production of yeast and hydrogen peroxide ...

    I will use this step with the other temperatures. I will then collect the data and write it in a results table. Volume of oxygen gas produced for each temperature given during the investigation. Temperature o C Vol. of oxygen gas given off (ml)

  1. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    Temperature (�C�1�C) Time (min) Control (Beads) ml�0.01ml Germinating Seeds ml�0.01ml Non-germinating Seeds ml�0.01ml 0 0 0.90 1.00 1.00 5 0.85 0.96 1.01 10 0.85 0.98 1.07 15 0.93 0.99 1.12 20 0.91 0.98 1.10 25 0.80 0.88 0.96 30 0.70 0.82 0.90 Raw Data Table 5: The volume of the water level

  2. Browning Enzyme

    Remember to label cuvettes, so you are able to identify the sample number and the temperature this is very important as if the cuvettes are not labeled this will lead to inaccuracies. 17) Then check for the absorbency and the transmittance.

  1. Biology- Extended essay. For this research, I investigated the effects of DDT and ...

    1 * Gloves � 1 pair * Goggles � 1pair PROCEDURE: I have divided my analysis into three separate primary investigations. The first investigation consists of the different concentrations of DDT; the second consists of the different concentration of the Azo Dye and the third which consists of a mixture of these two toxins.

  2. How does the salinity of water affect the germination of mung been seeds as ...

    However the exclusion of this data point would not have effected our graph as the mean would still be 3 seeds. Our results are explained by the science behind plant germination, and the factors which effect this process. For germination to occur the seed must have the correct abiotic conditions to begin.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work