• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How does the concentration of hydrogen peroxide affect the time (s) it takes the enzyme catalase to decompose it into water and oxygen gas?

Extracts from this document...


Biology Hl-Enzyme Design IA Elena Mastin Wood Research Question: How does the concentration of hydrogen peroxide affect the time (s) it takes the enzyme catalase to decompose it into water and oxygen gas? Variables: Dependent: 1. Time (in seconds) taken for the decomposition reaction to be complete. 1. Measured using a stopwatch Independent: 1. Molar concentration (mol/L) of hydrogen peroxide (H2O2) 1. Solutions will be increased in concentration from 1 mol/L to 1.25 mol/L, to 1.5 mol/L, to 1.75 mol/L, to 2 mol/L. Controlled: 1. Mass (in grams) ...read more.


1. Volume of hydrogen peroxide 1. A standard volume of 20mL of hydrogen peroxide will be used in each test, with ample amounts of water to change the concentration. 2. If it was measured using 20mL of the hydrogen peroxide solution, there would be less of the substance at lower concentrations, which would speed the reaction and possibly skew the results. 1. Container used 1. The same 250 mL beaker will be used for each test. 2. The surface area that is exposed will be kept constant in order to keep the area of reaction between the substrate and the enzyme constant, so as not produce inaccurate data. ...read more.


Stopwatch 4. 250mL beaker. Procedure 1. Measure 10g of pureed cow?s liver into a 250mL beaker. 2. Measure 20mL of 100% hydrogen peroxide and dilute to 1mol/L of hydrogen peroxide solution. 3. Pour the hydrogen peroxide solution into the beaker, and start timer. 4. Stop timer when the mixture ceases to bubble, signaling the end of the reaction. 5. Record time into a data table. 6. Repeat steps 1-5 with four more trials. 7. Repeat steps 1-6 with 1.25 mol/L of hydrogen peroxide solution. 8. Repeat steps 1-6 with 1.5 mol/L of hydrogen peroxide solution. 9. Repeat steps 1-6 with 1.75 mol/L of hydrogen peroxide solution. 10. Repeat steps 1-6 with 2 mol/L of hydrogen peroxide solution. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Browning Enzyme

    in the appearance of the discs yet not much could be done. These would lead the results to be larger than expected. When crushing the discs, some discs were thicker than others and this caused inaccuracies as when they were crushed in the crucible, the discs which were thicker, were

  2. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    2H2O2 ==> 2H2O + O2 2 Identify any anomalies or inconsistencies in your results. Students may identify some anomalies or inconsistencies in their results. They should be able to explain what to do with them. 3 Describe the shape of the graph.

  1. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    taken into account as it can affect the outcome of the investigation. Another uncertainty could be with the water bath that I used to make the temperature of the Hydrogen Peroxide solutions to 30�?. While the water bath that was used is electronically controlled and was set to 30�?, there

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    The pipettes each contain, germinating seeds, non-germinating seeds and bead. Temperature (�C�1�C) Time (min) Control (Beads) ml�0.01ml Germinating Seeds ml�0.01ml Non-germinating Seeds ml�0.01ml 21 0 0.68 0.88 0.72 5 0.65 0.82 0.68 10 0.65 0.76 0.65 15 0.64 0.75 0.62 20 0.63 0.71 0.60 25 0.63 0.70 0.58 30 0.62

  1. The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in ...

    deviation, T-value, Degrees of freedom and critical value for a comparison of mean relative rate of enzyme activity of catalase and enzyme catalase temperature of filter paper disks coated with enzyme catalase exposed to 3% hydrogen peroxide solution in culture tubes, Target Enzyme Catalase Temperature (�C)

  2. Testing the dissolved oxygen levels in water samples.

    The amount of carbon under the dark bag is respiration, while the amount of carbon under the transparent bag is the amount of photosynthesis minus the amount of respiration. There are three main gases dissolved in aquatic environments: nitrogen, oxygen, and carbon dioxide.

  1. Reaction Time

    However, whether the answers were correct or not was not considered in this investigation. Another research2 that has been done is that Japanese researchers tested 22 baseball players, 22 tennis players and 38 non-athletes. The results showed that baseball players scored better at a test which involved pressing a button

  2. Biology Lan - factors affecting enzyme activity

    After the contact, oxygen gas, the bubbles were formed and the gas was released into the graduated cylinder. Some amount of water went out the cylinder. The vial was agitated for 90 seconds for precise result. After 5 trials for each 5 beakers, all the materials were cleaned and they were all put back into the cabin.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work