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How does the concentration of hydrogen peroxide affect the time (s) it takes the enzyme catalase to decompose it into water and oxygen gas?

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Biology Hl-Enzyme Design IA Elena Mastin Wood Research Question: How does the concentration of hydrogen peroxide affect the time (s) it takes the enzyme catalase to decompose it into water and oxygen gas? Variables: Dependent: 1. Time (in seconds) taken for the decomposition reaction to be complete. 1. Measured using a stopwatch Independent: 1. Molar concentration (mol/L) of hydrogen peroxide (H2O2) 1. Solutions will be increased in concentration from 1 mol/L to 1.25 mol/L, to 1.5 mol/L, to 1.75 mol/L, to 2 mol/L. Controlled: 1. Mass (in grams) ...read more.


1. Volume of hydrogen peroxide 1. A standard volume of 20mL of hydrogen peroxide will be used in each test, with ample amounts of water to change the concentration. 2. If it was measured using 20mL of the hydrogen peroxide solution, there would be less of the substance at lower concentrations, which would speed the reaction and possibly skew the results. 1. Container used 1. The same 250 mL beaker will be used for each test. 2. The surface area that is exposed will be kept constant in order to keep the area of reaction between the substrate and the enzyme constant, so as not produce inaccurate data. ...read more.


Stopwatch 4. 250mL beaker. Procedure 1. Measure 10g of pureed cow?s liver into a 250mL beaker. 2. Measure 20mL of 100% hydrogen peroxide and dilute to 1mol/L of hydrogen peroxide solution. 3. Pour the hydrogen peroxide solution into the beaker, and start timer. 4. Stop timer when the mixture ceases to bubble, signaling the end of the reaction. 5. Record time into a data table. 6. Repeat steps 1-5 with four more trials. 7. Repeat steps 1-6 with 1.25 mol/L of hydrogen peroxide solution. 8. Repeat steps 1-6 with 1.5 mol/L of hydrogen peroxide solution. 9. Repeat steps 1-6 with 1.75 mol/L of hydrogen peroxide solution. 10. Repeat steps 1-6 with 2 mol/L of hydrogen peroxide solution. ...read more.

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