• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

How will changing the percentage of glucose concentration affect the light transmission percentage from the release of betacyanin caused by the permeability of cell membranes of beetroot cells?

Extracts from this document...


Claudia Cheng IA 3: Unit 2 SL/HL Investigating transport across membranes Version 2- The effect of a factor of cell membrane function Design and perform an experiment to determine the effect of a factor on the cell membranes. Introduction The cell membrane is the boundary that separates the internal parts of the cell from surroundings. Substances that move in or out of the cells must pass across the cell membrane. Beetroot (Beta vulgaris) cells contain red pigment called betacyanin. The red pigment is contained in a large vacuole inside the cells. The vacuole is separated from the cytoplasm of the cell by its own membrane. The membrane of the vacuole in a living cell does not normally let the red pigment through it. If the membranes of the cell are damaged, however, the red pigment may leak out of the vacuole and out of the cell. The extent of the damage to the membrane can be measured by the amount of red pigment escaping the cell. Research Question How will changing the percentage of glucose concentration affect the light transmission percentage from the release of betacyanin caused by the permeability of cell membranes of beetroot cells? ...read more.


Risk Assessment Lab coats and safety goggles would be worn during the experiment to prevent a lower risk of harmful chemicals to contact our skin Be cautious when using the core borer and knife as blades are sharp Long hair would be tied back Sandals and flip-flops would not be worn during the experiment. Shoes covering the feet have to be worn. Raw data results table Data collection and Processing Raw data table to show the effect of glucose concentration of cell membrane permeability Calculating standard deviation (of 20% concentration of glucose) Standard deviation = Standard deviation for 20% concentration of glucose: (37-35.2)2 + (35.2-35) 2 + (35.2-33) 2 + (39-35.2) 2 + (35.2-32) 2 = 32.8 SD= ? (32.8/5)= 2.6 Observations From observations, after the beetroot pieces was emerged into the solutions for around 10 minutes, I noticed that the solution with 100% glucose concentration was darker in colour (a crimson red) than the 20% glucose concentration one with a light pink colour. I also noticed that the lower concentration solutions such as 20-40% causes the beetroot to float up to the surface of the test tubes while the higher concentration solutions such as 80-100% would sink to the bottom. ...read more.


There are minor errors caused by the uncertainties from the equipment such as the measuring cylinders, which may cause my final reading to be inaccurate. Improve number of trials of experiment to reduce the human and systematic errors When using the long tweezers to clamp the beetroots out of the solutions after 10 minutes, excess pigment may have been released due to the pressure on the beetroot from the tweezers. This may also affect the light transmission percentage, which may cause final results to be inaccurate. Pour solution into another test tube instead of clamping the beetroot out so there won?t be excess pigment coming out of the beetroot. From the data results table, the difference between each average light transmission percentage isn?t that big. This means that there was not a lot of pigment released. This may be caused by not having the beetroot pieces in the glucose solutions long enough for more pigment to be released. Leave the beetroot pieces in the solutions for a longer period of time (20 to 25 minutes) to make sure the change in colour is has a bigger difference so results may be easier to interpret. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. The effect of concentration of sucrose solution on the osmosis in potato

    Diagram 12: The different % of sucrose solution used in the experiment.

  2. Biology Lab - frequency of cell division in animal and plant cell

    In addition, some of the high-power objective lenses did not work for some microscopes. Thus, we had to use medium-power to view the cells and this prevented us from having clear view of cells. Also, we were limited in number of slides because we could only see one type of

  1. Testing the solute concentration of potato cells

    Even so, potatoes were all together browned. This would still make the comparison clear, then. Seeing the standard deviation of 0.2 cm, the precision can be thought high. All five trials displayed very close result to each other. And five trials were many enough at least for calculating standard deviation.

  2. The effect on osmosis on the egg shell

    EVALUATION I think that the research question was clearly formulated as the independent and dependent variables were included and the hypotheses were testable. Although hypotheses were right, it should be specified that only higher salt solution (as 10%) changes the direction of osmosis.

  1. Biology Lab Design Glucose concentration

    Water bath temperature �C The temperature has to be the same to ensure a fair test as the rate of reaction could be different if the temperatures are different. Moreover, the test for reducing sugar is an enzymatic reaction. If the samples are heated at too high a temperature, the enzymes present would become denatured.

  2. Effect of Detergent on Membrane Permeability

    there is more time for the plasma membrane to be penetrated Every sample beetroot pieces stay in the solution for an equal amount of time; 10 minutes. Only one person does the timing to avoid difference in reaction times between humans.

  1. Beet Lab. Aim: To determine the effects of temperature on the permeability of ...

    {2} A spectrophotometer can measure the absorbance of a solution. As more betacyanin leaks out into the extracellular luid, the solution will absorb more light. Since the spectrophotometer reading is proportional to the leakage of pigments from the beet cells, the absorbance of the solution can be used to evaluate the level of permeability of the membrane in beet cells.

  2. The Effect of Alcohol on Biological Membranes

    Immediately drain off the water. This will wash off any pigment released during the cutting process. 9. Set the timer to 10 minutes and begin timing. Use forceps to place a piece of beet into each of 15 wells, as shown in Figure 1.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work