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How will changing the percentage of glucose concentration affect the light transmission percentage from the release of betacyanin caused by the permeability of cell membranes of beetroot cells?

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Introduction

Claudia Cheng IA 3: Unit 2 SL/HL Investigating transport across membranes Version 2- The effect of a factor of cell membrane function Design and perform an experiment to determine the effect of a factor on the cell membranes. Introduction The cell membrane is the boundary that separates the internal parts of the cell from surroundings. Substances that move in or out of the cells must pass across the cell membrane. Beetroot (Beta vulgaris) cells contain red pigment called betacyanin. The red pigment is contained in a large vacuole inside the cells. The vacuole is separated from the cytoplasm of the cell by its own membrane. The membrane of the vacuole in a living cell does not normally let the red pigment through it. If the membranes of the cell are damaged, however, the red pigment may leak out of the vacuole and out of the cell. The extent of the damage to the membrane can be measured by the amount of red pigment escaping the cell. Research Question How will changing the percentage of glucose concentration affect the light transmission percentage from the release of betacyanin caused by the permeability of cell membranes of beetroot cells? ...read more.

Middle

Risk Assessment Lab coats and safety goggles would be worn during the experiment to prevent a lower risk of harmful chemicals to contact our skin Be cautious when using the core borer and knife as blades are sharp Long hair would be tied back Sandals and flip-flops would not be worn during the experiment. Shoes covering the feet have to be worn. Raw data results table Data collection and Processing Raw data table to show the effect of glucose concentration of cell membrane permeability Calculating standard deviation (of 20% concentration of glucose) Standard deviation = Standard deviation for 20% concentration of glucose: (37-35.2)2 + (35.2-35) 2 + (35.2-33) 2 + (39-35.2) 2 + (35.2-32) 2 = 32.8 SD= ? (32.8/5)= 2.6 Observations From observations, after the beetroot pieces was emerged into the solutions for around 10 minutes, I noticed that the solution with 100% glucose concentration was darker in colour (a crimson red) than the 20% glucose concentration one with a light pink colour. I also noticed that the lower concentration solutions such as 20-40% causes the beetroot to float up to the surface of the test tubes while the higher concentration solutions such as 80-100% would sink to the bottom. ...read more.

Conclusion

There are minor errors caused by the uncertainties from the equipment such as the measuring cylinders, which may cause my final reading to be inaccurate. Improve number of trials of experiment to reduce the human and systematic errors When using the long tweezers to clamp the beetroots out of the solutions after 10 minutes, excess pigment may have been released due to the pressure on the beetroot from the tweezers. This may also affect the light transmission percentage, which may cause final results to be inaccurate. Pour solution into another test tube instead of clamping the beetroot out so there won?t be excess pigment coming out of the beetroot. From the data results table, the difference between each average light transmission percentage isn?t that big. This means that there was not a lot of pigment released. This may be caused by not having the beetroot pieces in the glucose solutions long enough for more pigment to be released. Leave the beetroot pieces in the solutions for a longer period of time (20 to 25 minutes) to make sure the change in colour is has a bigger difference so results may be easier to interpret. ...read more.

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