• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

internal assessment enzyme

Extracts from this document...

Introduction

NAME : ZUBAIR ABDUR RAHIM BIN AHMAD CLASS : M08L TITLE : ENZYME ACTIVITY RESEARCH QUESTION What is the effect on different temperature on the rate of reaction of amylase enzyme? INTRODUCTION Enzymes are biological catalyses; most of them are protein. A catalyst speeds up the rate of reaction of a chemical reaction without itself being changed at the end of the reaction. A few properties of protein are - enzymes are extremely efficient, extremely specific, can be denatured by heat (temperature) and are affected by pH. HYPOTHESIS As the temperature of the starch and amylase increase, the rate of the enzyme reaction is getting higher. However, when the solution reaches beyond the optimum temperature which is high, the rate of enzyme reaction is getting lower and eventually stopped due to the denaturation of the enzymes. VARIABLES Independent : Temperature of the water bath by varying the temperature for each experiment from 5�C, 20�C, 26�C, 50�C, 70�C and 100�C. ...read more.

Middle

�0.5 Time taken for the starch to completely hydrolyse , s � 0.1 min First reading Second reading Control experiment 5.0 42 44 ? 20.0 36 34 ? 26.0 20 18 ? 50.0 26 22 ? 70.0 38 34 ? 100.0 ? ? ? Table 1 : the time taken for starch to hydrolyse completely Table 1 : time taken for starch to be hydrolysed completely in different temperature DATA PROCESSING The average reading from 2 sets of experiment is taken using the formula Temperature, �C �0.5�C Average time taken, t �0.1 minutes 5.0 43 20.0 35 26.0 19 50.0 24 70.0 36 100.0 Control experiment Table 2 : the average reading for the time taken to completely hydrolyse starch The rate of enzymatic reaction of amylase enzyme can be calculated using the formula : where `t' is the time taken for starch to completely hydrolyse. Uncertainties of rate of reaction, ?R = � R Where, t = time taken for iodine to change its colour from dark blue to brown ?t = uncertainties of t ...read more.

Conclusion

The hypothesis is accepted as the temperature of starch and amylase enzyme increase the rate of reaction will be increased until the optimun temperature and the rate of reaction decreased when the temperature of mixture of amylase enzyme and starch is above the optimum temperature. WEAKNESSES SOLUTION The temperature of water bath is not accurate because the temperature is very unstable as it is only maintained manually. We have to use the electronic water bath in order to maintain the temperature required for the experiment. The iodine solution is oxidised when the solution is put into the white tile too early and this affect the colour of mixture of starch and iodine. The iodine must be put into the white tile only when we want to test the presence of starch in the mixture of amylase enzyme and starch. Some of the starch solution are deposited at the bottom of the test tubes during the experiment and this situation cause the reaction not to react completely Stir the solutions in the test tubes well by using the glass rod. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Osmosis Internal Assessment (Biology)

    4 star(s)

    Temperature of distilled water All test tubes containing the potato strips immersed in distilled water are left in an air-conditioned room over the period of five hours set at the temperature 23� Celsius, which is monitored every hour to ensure that it remains constant Exposure to surrounding environment All of

  2. Browning Enzyme

    30 �C 0.293 40�C 0.344 50 �C 0.420 60�C 0.301 70 �C 0.040 Standard Deviation without anomalies Temperature � 0.1 �C Standard Deviation (2 s.f.) 30 �C 0.011 40�C 0.035 50 �C 0.055 60�C 0.012 70 �C 0.009 The values in orange are values that have been reduced as an a result of excluding and ignoring the anomalies.

  1. internal assessment rate of transpiration

    According to graph 1, the rate of transpiration is higher during low intensity of light as compared to rate of transpiration during high intensity of light. This observation does not agree with theory stated before. There are other possibilities to cause this rate of transpiration to occur.

  2. Biology Internal Assessment Peas Lab

    Solution: Next time use a specific size of beaker, fill it completely up (don't forget to measure the mass of the beaker itself), take the mass of it and subtract the mass of the beaker from it. This way we make it as close as possible to having the equal mass of both types of peas inside of the flasks.

  1. Should Animals have the same rights as Humans? Both animals and humans exhibit behaviours ...

    at times. However, a glance at any dating page from a newspaper or online will show that both men and women are setting out clear (and broadly similar) criteria for prospective partners. One interesting difference is that whereas in the animal world (especially the bird kingdom)

  2. ProblemWhat kind of roles the factors of pH levels and temperature have on various ...

    * Salt concentration plays a huge role on the effectiveness of enzymes. If the slat concentration is close to zero, the enzyme will denature and form and inactive precipitate. If the salt concentration is very high, normal interaction will be blocked.

  1. Experiment-Effect of Temperature on the Action of Amylase Enzyme

    of amylase enzyme activity because it is the optimum temperature for enzymes performance. When it begins to digest food in our body its environment is supposed to match that. The rate of amylase enzyme activity decrease as the temperature decreases until 85°C.

  2. Biology Internal Assessment - investigate whether the Window and Notching caterpillar share the same ...

    midrib of the cabbage tree leaf to the nearest point of the notched and window feeding site can be accepted. Meaning I am 95% certain that there is a significant difference between the two means, and therefore there is a significant difference in distance from the midrib of the cabbage

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work