internal assessment germination

If the germination process occurs, there will be changes on the mass of the seed. During seed germination, the seed will use up the storage center (food reserve) inside the cotyledon which result in decreasing mass of the seed and then, after it is able to conduct photosynthesis, the mass will increase again because it will then develop new shoots and roots.

Variables

1. Independent – environment and condition of the seed during germination process.  One set of experiment is provided with water while the other set is leaved without having water.
2. Dependent – changes in the mass of the seed from day to day is being measured using electronic balance.
3. Controlled –

1. type of seed  - green bean seed is used throughout the experiment
2. Temperature – the condition of both set of experiment is set to under standard room temperature.
3. Presence of oxygen – because they are placed at the same spot, they will obtain the same quantity of oxygen.
4. Light intensity – placed at the same place, both of the set is having the same light intensity throughout the experiment.
5. Distance between the seed – in order to make sure the competition rate between the seed is minimum; they are placed at equal distance from each other in both set of experiment.

Materials and Apparatus

table 1; the materials and apparatus needed

Method

1. Choose 42 green beans of good quality – without any significant defect- and equally divide them into two groups of 21 beans each.
2. Place each group of beans in a beaker and label them as group A and group B.
3. Soak group A beans overnight by adding tap water inside the beaker until all the beans are completely submerged.
4. Prepare two petri dish and label them as A and B. put a moist cotton wool in petri dish A while petri dish B is left without any cotton wool.
5. Put the beans in beaker A uniformly into petri dish A and the beans in beaker B evenly into petri dish B.
6. Leave the beans overnight and the next day, pick 3 beans from each petri dish and remove any plumules or radicles from the beans.
7. Place the beans from petri dish A and B inside a microwave oven for 20 seconds at 300c.
8. Measure and record their respective mass using an electronic balance.
9. Repeat step 6 – 8 each day. Make sure that the cotton wool inside petri dish A is always moist.

Data collection

Table 2 ; the mass of 3 green beans for each day