Investigation into the Enzyme Activity of Catalyze in the Decomposition of Hydrogen Peroxide while exposed to different substrate concentrations

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Investigation into the Enzyme Activity of Catalyze in the Decomposition of Hydrogen Peroxide while exposed to different substrate concentrations

Student: Nico Palesch

Course: Biology HL IB 11

Teacher: Mrs. Hardy

School: Nelson Mandela School Berlin

Procedure Background

In this pre-designed procedure the enzyme activity of catalase in a potato was tested, in the form of measuring the volume of the Oxygen (O2) released by a catalase - hydrogen peroxide (H2O2) reaction in the span of one minute. The more oxygen released by the reaction the higher the enzyme activity was assumed to be, as more of the product was released in the same period of time.

The variable to be tested was the substrate concentration of the reaction, in this case the hydrogen peroxide. In each set of trials more hydrogen peroxide was added to the reaction, in order to increase the substrate concentration, but in each set the time limit was strictly one minute (+/- 0.1 miliseconds), to make sure that the resulting oxygen volume was a reflection of the enzyme activity, and not simply a reflection of the amount