• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation into the Enzyme Activity of Catalyze in the Decomposition of Hydrogen Peroxide while exposed to different substrate concentrations

Extracts from this document...

Introduction

Data Collection and Processing & Conclusion and Evaluation Investigation into the Enzyme Activity of Catalyze in the Decomposition of Hydrogen Peroxide while exposed to different substrate concentrations Student: Nico Palesch Course: Biology HL IB 11 Teacher: Mrs. Hardy School: Nelson Mandela School Berlin Procedure Background In this pre-designed procedure the enzyme activity of catalase in a potato was tested, in the form of measuring the volume of the Oxygen (O2) released by a catalase - hydrogen peroxide (H2O2) reaction in the span of one minute. The more oxygen released by the reaction the higher the enzyme activity was assumed to be, as more of the product was released in the same period of time. The variable to be tested was the substrate concentration of the reaction, in this case the hydrogen peroxide. In each set of trials more hydrogen peroxide was added to the reaction, in order to increase the substrate concentration, but in each set the time limit was strictly one minute (+/- 0.1 miliseconds), to make sure that the resulting oxygen volume was a reflection of the enzyme activity, and not simply a reflection of the amount of hydrogen peroxide acted upon. ...read more.

Middle

Qualitative Data - In set one, where 0.50 ml of hydrogen peroxide was added, the column of oxygen rose unevenly, as the bubbles rise faster on one side of the cylinder than the other. - In all sets the color of the middle of the column of oxygen was a distinct yellow, and at both the top and bottom ends was a faded orange. - The more hydrogen peroxide used in each trial, the faster the column would rise. - Initially, in approximately the first 5 seconds after adding the hydrogen peroxide, the column of oxygen bubbles rose minimally. After this first phase the rate would speed up, and consistently rise at the same pace until about a minute had gone by, at which point the rate of rising fell again. - After the reaction had taken place the pieces of potato used remained intact, but the surface consistently turned black. Calculating the Average As can be seen in the above table, for each set of 5 trials with a different amount of hydrogen peroxide used there were 5 separate, and usually similar results. ...read more.

Conclusion

Therefore, the calculations for Set 4 looked like this: 35 + 37 + 34 + 33 = 139 139 : 4 = 34.75 � 34.8 Calculating the Standard Deviation The next step in the statistical analysis of the data was to find the Standard Deviation (SD) of each set of trials. The reason for finding the SD is to be able to see how much the results tend to diverge from the average, and therefore be able to see how accurate and consistent your results are. If there is a small SD it means the results tend to be close to the average result, meaning that they are all relatively close together. If the SD is a large number, it means the results are far away from each other, meaning your results are not that consistent. In order to find the SD I used the following formula: The following table shows the standard deviation of the different sets of trials. Amount of Hydrogen Peroxide (ml +/- 0.05 ml) used in Set of Trials Standard Deviation of the Set of Trials 0.50 1.1 0.75 2.04 1.00 1.7 1.25 1.7 1.50 2.5 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Peer reviewed

    Enzyme Coursework. Investigation to find the effect of substrate concentration on the rate ...

    5 star(s)

    Time taken (seconds) #1 #2 #3 0.20 63.0 65.0 58.0 0.40 34.0 34.3 33.8 0.60 18.0 18.3 18.1 0.80 12.2 12.0 12.3 1.00 8.2 8.0 8.2 Processed data Table #2: Mean time taken for the pieces of paper to rise Concentration of hydrogen peroxide solution (mol/dm-3) Mean time taken (seconds)

  2. Browning Enzyme

    try to even out the volume in the cuvette, to keep the experiment fair and accurate. The fluctuation may have occurred due to the fact there were some droplets of solution around the rim of top of cuvette, this can resolved by using a pipette to pour the solution in carefully.

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    After 16% the increase in the rate of reaction slows down. This is shown by the gradient of the graph going down. At this point virtually all the active sites are occupied so the active sites are said to be saturated with Hydrogen Peroxide.

  2. The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in ...

    These significant differences further support the hypothesis as it illustrates that temperatures above the optimal (37�C) by a huge amount (ex. Temperatures that are greater than 40�C) incredibly lower and/or even completely stop enzyme reaction rate activity due to denaturation of high temperatures.

  1. Transpiration Investigation

    5452 460 104.9 104.3 104.6 5174 5681 5427 470 104.9 104.3 104.6 5179 5687 5433 480 104.8 104.2 104.5 5193 5596 5395 490 104.8 104.1 104.4 5236 5725 5481 500 104.7 104.0 104.4 5192 5675 5434 510 104.7 104.0 104.3 5179 5681 5430 520 104.6 103.9 104.3 5089 5766 5427

  2. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    The last of the test tubes will not have the Hydrogen Peroxide change in pH level (through my preliminary work, I have found out that the pH level of the Hydrogen Peroxide, 10 % to be approximately pH 7.5. This was found out through the use of the pH probe).

  1. How does the concentration of hydrogen peroxide (substrate) affect the enzymatic activity of catalase ...

    12ml: 8ml = 60% 4. 16ml: 4ml = 80% 5. 20ml: 0ml= 100% Dependent variable The volume of oxygen produced (cm3) The volume of oxygen produced from the reaction between the carrot catalase and the hydrogen peroxide will be collected and measured using a gas syringe.

  2. An investigation of the effect enzyme concentration has on the rate of catalase activity ...

    The amount of water in the graduated cylinder is always filled to the top because if it wasn?t filled then the volume recorded may not be as a result of the oxygen bubbles but as a result of a partially empty graduated cylinder.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work