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Is the rate of passive transport through potato slices and saltwater and distilled water significantly different?

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Introduction

´╗┐Haley Qualman IB Bio 3 Potato Lab Write Up Background: Passive transport does not require energy, moves from an area of high concentration to a low, and goes along a concentration gradient. Osmosis is a type of passive transport that moves across a semi-permeable membrane through water. Research Question: Is the rate of passive transport through potato slices and saltwater and distilled water significantly different? Hypothesis: If potato slices are placed in both saltwater and distilled water and their mass is recorded before and after, then the potato in distilled water will have a greater rate of diffusion, but there will be no significant difference. Variables: Independent: Time in solute (saltwater) Dependent: Rate of absorption Controlled: The balance, the person putting the slices on the balance, amount of salt, amount of distilled water, number of slices, number of times experiment repeated, temperature Equipment List: 1. ...read more.

Middle

Blot excess water out. 7. After 21 hours, take the potatoes out and place them in the correct bag 8. Collect the mass of each bag and record. 9. Find the difference, the rate, and use a T-Test to determine significance. Raw data: Salt Water (NaCl) before Salt Water (NaCl) after Difference Rate Cup 1 4.20g 4.27g .07 0.00333 Cup 2 4.78g 5.14g .36 0.01714 Cup 3 4.33g 4.46g .13 0.00619 Cup 4 4.32g 4.51g .19 0.00905 Cup 5 4.05g 4.33g .28 0.01333 Distilled Water before Distilled Water after Difference Rate Cup 1 4.29g 5.65g 1.36 0.06476 Cup 2 4.27g 5.98g 1.71 0.08143 Cup 3 4.38g 5.99g 1.61 0.07667 Cup 4 4.24g 5.81g 1.57 0.07476 Cup 5 4.25g 5.63g 1.38 0.06571 Data Processing: Overview: To calculate the rate, find the difference and divide by the number of hours (21). ...read more.

Conclusion

When salt was added, the potato had more solutes surrounding it, making the concentration smaller. This made the rate smaller, making the difference in weight smaller. The hypothesis made is incorrect. Limitations of Experimental Design: The experiment could?ve been conducted in many different ways, through volume, length, or anything other than mass. The mass of salt and potatoes could?ve been not very precise and the amount of water could?ve been a mL or two off. The salt may have mixed in differently, the time could?ve been off by a few minutes, and the bag weight could be different. Suggestions for Improvement: If the investigation had to be redone, a different measure could be used instead of mass, there could be more data in each set, the measurements would?ve been more exact, and the bag weight would be taken off. More salt could be added to see if the weight would actually decrease more. Reference: Damon, Alan, McGonegal, Randy, & Ward, William. Biology. Pearson Education Limited, 2007. ...read more.

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