• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Lab 2 : Microscopy OBJECTIVES Describe how to care for a compound light microscope Recognize and give the function of the parts of a compound light microscope Accurately align a compound light microscope

Extracts from this document...


TITLE : Lab 2 : Microscopy OBJECTIVES 1. Describe how to care for a compound light microscope 2. Recognize and give the function of the parts of a compound light microscope 3. Accurately align a compound light microscope 4. Correctly use a compound light microscope MATERIALS 1. Compound light microscope 2. Lens paper 3. Labeled diagram of the compound light microscope METHODS 1. The seat and microscope is adjusted according to the proper height so that usage of the microscope is comfortable. 2. The light source is then turned on with a 50% illumination and the 4 x objective is in the light path. An audible click was heard when the slot is in position. The condenser is then moved as high as possible and then backed down 1/4 turn of the wheel. The diaphragm is then adjusted so that the illumination is appropriate. 3. A letter slide is then placed on the stage and secured with clamps; the letter slide is adjusted to reading position. ...read more.


But when the condenser is moved far away, the image disappears and cannot be seen. 9. Next, the 10x objective lens is rotated into the light path and the fine focus knob is used to adjust the display of the image. The degree of the detail seen in the letter 'I' becomes slightly blurred as the image had to be focused again. 10. When the diaphragm is open, it is not possible to see the actual strands with the paper either. By moving the slide slowly to the right, the image moves to the left as the image is always inverted. 11. There was very slight difference when the image was viewed with the 4x objective and the 10x objective as the image was only magnified. 12. The next step was to carefully and slowly rotate the 40x objective into place, by doing so, the image is not in focus neither is the illumination of the image adequate when only the fine adjustment is adjusted. ...read more.


2. Never wipe glass lens with anything other than lens paper. 3. Always remember that microscope is a precious instrument. Never force any of its Moving parts. 4. Clean dirty slides with lens paper before using. 5. One end of a glass cover slip must be placed either to the right or left of the specimen so that the rest of the slip is held at a 45� angle over the specimen. 6. Slowly lower the cover slip with a dissecting needle to avoid trapping air bubbles. CONCLUSION In short, microscope is a precious instrument. Therefore, we have to handle it with care especially when we carry a microscope to and from the lab bench. Always grasp the arm with dominant hand and support the base with the other hand. Condenser focused the visible light from the light source and pass through the specimen on the slide, then through the objective lens and lastly the ocular lens. From the whole experiment, the idea was to be able to learn to adjust and handle the microscope with better knowledge and care. REFERENCE http://en.wikipedia.org/wiki/Microscopy http://www.ou.edu/research/electron/www-vl/ ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Plant Tropism Lab

    would grow heavily to the right the next day, maintaining their upright position. If one was to look at Charts 3.1 - 3.5, one would find a sharp increase in bend degree on March 17, the day after the beans were rotated 90 degrees to the left.

  2. Digestion Lab discussion

    temperature and 40�C body temperature but will not digest at a temperature greater than or equal to 75�C.

  1. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    was 0.00061 ml/min and one in ice water (5�C�1�C) was 0 ml/min According to the graph, the slope of the oxygen consumption and the rate of oxygen consumption of germinating seeds in both room temperature and the iced water are steeper than the ones for non-germinating seeds.

  2. Biology lab. Observing plant and animal cells using the light microscope

    B-Cell of banana pulp: 1.Using a scalpel cut a slice of banana. Observe the skin with its prominent ridges and the central part which surrounds the pulp. 2. Take a very small sample of pulp (the size of a pin head)

  1. Biology Industrial Melanism of Peppered Moth Lab

    X1 = Mean in Sample 1 X2 = Mean in Sample 2 N1 = # in Sample 1 N2 = # in Sample 2 S21 = Variance in Sample 1 S22 = Variance in Sample 2 t= 88.0-51.3 V( 5-1)(70.1)

  2. Osmosis Lab

    Gather and prepare all the items from the list of apparatus'. 2. Fill 5 beakers with 500ml of distilled water each. 3. For the first set of results (5 beakers), no salt is needed to be placed into the beaker.

  1. Enzyme Lab

    Time The time in which we let our reaction react was a time span of 2 minutes. By keeping this constant time span, we were able to get accurate results of the amount of reaction in that certain time span.

  2. Biology lab - testing the effects of light intensity on photosynthesis.

    In the other two test tubes, place 1 ml of aerated water. Mark these as test tubes 3 and 4.. 6. Place test tubes 1 and 2 in the test rack. 7. Place aluminum foil the sides of the test tubes 2 and 4.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work