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Lab 2 : Microscopy OBJECTIVES Describe how to care for a compound light microscope Recognize and give the function of the parts of a compound light microscope Accurately align a compound light microscope

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Introduction

TITLE : Lab 2 : Microscopy OBJECTIVES 1. Describe how to care for a compound light microscope 2. Recognize and give the function of the parts of a compound light microscope 3. Accurately align a compound light microscope 4. Correctly use a compound light microscope MATERIALS 1. Compound light microscope 2. Lens paper 3. Labeled diagram of the compound light microscope METHODS 1. The seat and microscope is adjusted according to the proper height so that usage of the microscope is comfortable. 2. The light source is then turned on with a 50% illumination and the 4 x objective is in the light path. An audible click was heard when the slot is in position. The condenser is then moved as high as possible and then backed down 1/4 turn of the wheel. The diaphragm is then adjusted so that the illumination is appropriate. 3. A letter slide is then placed on the stage and secured with clamps; the letter slide is adjusted to reading position. ...read more.

Middle

But when the condenser is moved far away, the image disappears and cannot be seen. 9. Next, the 10x objective lens is rotated into the light path and the fine focus knob is used to adjust the display of the image. The degree of the detail seen in the letter 'I' becomes slightly blurred as the image had to be focused again. 10. When the diaphragm is open, it is not possible to see the actual strands with the paper either. By moving the slide slowly to the right, the image moves to the left as the image is always inverted. 11. There was very slight difference when the image was viewed with the 4x objective and the 10x objective as the image was only magnified. 12. The next step was to carefully and slowly rotate the 40x objective into place, by doing so, the image is not in focus neither is the illumination of the image adequate when only the fine adjustment is adjusted. ...read more.

Conclusion

2. Never wipe glass lens with anything other than lens paper. 3. Always remember that microscope is a precious instrument. Never force any of its Moving parts. 4. Clean dirty slides with lens paper before using. 5. One end of a glass cover slip must be placed either to the right or left of the specimen so that the rest of the slip is held at a 45� angle over the specimen. 6. Slowly lower the cover slip with a dissecting needle to avoid trapping air bubbles. CONCLUSION In short, microscope is a precious instrument. Therefore, we have to handle it with care especially when we carry a microscope to and from the lab bench. Always grasp the arm with dominant hand and support the base with the other hand. Condenser focused the visible light from the light source and pass through the specimen on the slide, then through the objective lens and lastly the ocular lens. From the whole experiment, the idea was to be able to learn to adjust and handle the microscope with better knowledge and care. REFERENCE http://en.wikipedia.org/wiki/Microscopy http://www.ou.edu/research/electron/www-vl/ ...read more.

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