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Liver Lab

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Introduction

LIVER Introduction The adult human liver normally weighs between 3 - 6 pounds; it is a soft, pinkish-brown boomerang shaped organ. It is the second largest organ and the largest gland in the human body. Its position in the body is right under the ribcage on the right side of the upper abdomen. The liver lies on the right of the stomach and makes a kind of cradle for the gallbladder. The liver has four important jobs, production of bile to help digestion, cleaning up toxic substances and dead cells (detoxification), storing essential proteins, carbohydrates, vitamins and minerals (metabolism), and production of essential proteins and substances required for body functions. Food is made up of carbohydrates, proteins, fats, vitamins and minerals. When we eat, food enters the stomach where the important phase of digestion occurs. Then the food passes on to small intestine where the main work of digestion takes place with the help of liver. Liver makes bile and digestive enzymes, special proteins that help to break down food to give energy. Bile is made by liver but is stored in gall bladder. Many waste products are unseen by bile before leaving the body as body waste. In metabolism liver cells store carbohydrate as glycogen, which either comes straight from diet or is made from other sources of food. ...read more.

Middle

--> Slowest If the liver enzyme was put into H2O2 at 25�C, then the mixture would bubble until the liver enzyme disappears. --> Slow If the liver enzyme was put into H2O2 at 40�C, then the mixture would bubble so much the liver enzyme would bubble right out of the test tube. --> Fast If the liver enzyme was put into H2O2 at 65�C, then the mixture would explode blowing the enzyme right out of the test tube. --> Fastest Materials 1. -5�C liver 2. 25�C liver 3. 40�C liver 4. 65�C liver 5. Test tube 6. H2O2 7. Stick 8. Paper Experiment **Make four round paper disks (the small round remains from whole puncher)** Put -5�C liver on the paper disk then push it down to the bottom of the H2O2 filled test tube. Calculate the time that it takes to rise up, also record the rise motion and H2O2 reaction. Put 25�C liver on the paper disk then push it down to the bottom of the H2O2 filled test tube. Calculate the time that it takes to rise up, also record the rise motion and H2O2 reaction. Put 40�C liver on the paper disk then push it down to the bottom of the H2O2 filled test tube. Calculate the time that it takes to rise up, also record the rise motion and H2O2 reaction. ...read more.

Conclusion

Mt hypothesis that stated, if the liver enzyme was put into H2O2 at 65�C, then the mixture would explode blowing the disk right out of the test tube, was true. The reaction at this temperature was apparent even before the disk got a quarter of the way down the tube. It took us forever to get the disk to the bottom of the tube, because the hydrogen peroxide kept oozing out as the liver touched it. Liver enzymes reactions slow down and last longer when they are in acidic medium. But on the other hand, enzymes reactions are faster in basic medium, but still last longer. The best reaction is at about a neutral pH on its own environment of liver, it is a quick time very rapid reaction. This experiment that I constructed showed the speeds of enzymes at various temperatures. The cold enzymes reacted slowly with the acidic hydrogen peroxide. And the hot enzymes reacted very quick and rapidly with the acidic hydrogen peroxide. What if we weren't using acidic stuff like hydrogen peroxide, and instead we were using a basic medium. My hypothesis is that the cold enzyme would be crazy fast, and the hot would be unimaginable. I think this experiment shows what happens to food we eat, because your livers enzymes have to clean and help digest what we eat. So if we eat acidic food enzymes have to work harder, than when we eat basic food; and when we eat food of neutral pH the enzymes have a better medium for reactions. ...read more.

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