LR Biology liver enzyme

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LAB REPORT: BIOLOGY

Title: Cow liver catalase experiment.

Question: - Is there enzyme activity?

-How is the rate of the following reaction with a low temperature compared to a normal temperature?

2H2O2 (catalase as an enzyme) --> 2H2O + O2

Materials: 1000ml of distilled water, 50g of Cow liver, 1x Bunsen burner, 1x Thermometer, 1x Syringe (1 ml ± 0.1ml), Tap water, 1x Syringe needle, 50ml of water peroxide (10vol), 1x Stopwatch, 1x Erlenmeyer flask, 1x Measuring cylinder (ml ± 1 ml), 2x Water bath, 1x Thin rubber tube.

Variables:

Independent: Time (by stopwatch, ± 3s)

Dependent: Amount of O2 released

Controlled: Concentration of catalase solution (50g blended in 1000ml of distilled water), amount and concentration of Water Peroxide (50ml, 10vol), amount of catalase added (1ml in syringe ± 0.1ml), initial temperature of the catalase solution, Temperature ± 1ºC, data values (by the same observer with equal/indifferent criteria of judging).

Not controlled: Amount of tap water added and number of ice cubes.

Diagram:

Procedure:

- A solution of 50g of catalase per 1000ml of water was prepared.

- The Erlenmeyer flask was placed inside a