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MICROMETRY- Calculating actual width of cell wall:

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Introduction

LE BOCAGE INTERNATIONAL SCHOOL MICROMETRY Practical 1 Mahmood Zabioullah Fokeerbux 3/24/2009 Question 1: Electron micrograph 1 RAW DATA COLLECTED Magnification= X16, 000 Record Magnified width of the cell wall (�1mm) 1 3mm 2 3mm 3 4mm 4 4mm 5 3mm PROCESSING DATA Mean of records= (3mm+3mm+4mm+4mm+3mm)/5 =17mm/5 =3.4mm �3mm Standard Deviation=0.55 Range=4mm-3mm =1mm Calculating actual width of cell wall: Magnification= size of drawing/ size of object Magnification= X16, 000 Average width of magnified cell wall= 3mm Actual width of cell = size of object/ magnification =3mm/16, 000 =0.0001875mm =0.1875�m =187.5nm �188nm EVALUATION The standard deviation is very low showing that the values recorded are very close to the mean (clustered to the mean). ...read more.

Middle

Question 2: Electron micrograph 2 1) Diameter of the nucleolus RAW DATA COLLECTED Magnification= X25, 000 Record Magnified diameter of nucleolus (�1mm) 1 76mm 2 77mm 3 77mm 4 77mm 5 76mm PROCESSING DATA Mean of records= (76mm+77m+77mm+77mm+76mm)/5 =383mm/5 =76.6mm �77mm Standard Deviation=0.55 Range=77mm-76mm =1mm Calculating actual width of cell wall: Magnification= size of drawing/ size of object Magnification= X25, 000 Average width of magnified cell wall= 77mm Actual width of cell = size of object/ magnification =77mm/25, 000 =0.00308mm =3.08�m �3.�m EVALUATION The values recorded were very consistent, thus the range was not too large. ...read more.

Conclusion

Calculating actual length of nucleus: Magnification= size of drawing/ size of object Magnification= X25, 000 Average width of magnified cell wall= 207mm Actual width of cell = size of object/ magnification =207mm/25, 000 =0.00828mm =8.28�m �8�m EVALUATION Mean, standard deviation and range could not be found as only 1 value was considered. With better equipments, the values could have been recorded with more precision. 3) Width of nuclear envelope RAW DATA COLLECTED Magnification= X25, 000 Record Magnified width of nuclear envelope (�1mm) 1 1mm 2 1mm 3 0.75mm 4 1mm 5 0.50mm ?? ?? ?? ?? ...read more.

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