• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Osmosis Lab. Aim: To investigate the effects of different solute concentrations on osmosis, calculate water potential, and plant cell plasmolysis.

Extracts from this document...


Osmosis Lab Aim: To investigate the effects of different solute concentrations on osmosis, calculate water potential, and plant cell plasmolysis. Hypothesis: It is hypothesized that if in osmosis, water were moving out of the cell into higher concentrations then when the osmotic pressure increases the mass of the cells would shrink and decrease. I believe that the solutions with the higher amount of sucrose solutions will end up having the highest osmotic pressure along with the smallest mass meaning that the cells went through osmosis and shrunk the cells of the potato core. Variables: Independent/Experimental: Size of Potato Core Dependent: Change of Mass Controlled: Measuring Mass, Measuring Temperature Procedure: 1. Cut 4 potato cylinders with the cork borer for each solution that you will use, and then cut them evenly so that they all are 3 cm in length. Remove any potato skin. 2. Pour 100 mL of each sucrose solution that has already been prepared (water, 0.2M, 0.4M, 0.6M, 0.8M and 1.0M) in a cup and label it. 3. Add 4 potato cylinders to each solution cover it and let it stand overnight. ...read more.


The degrees would stay the same in both days but as you can see there is a change is the data for mass from day one to day two. The osmotic pressured was also measured causing the loss of mass in the potato. Osmotic Pressure Constant 0 Potato 0.2 -0.39888 Potato 0.4 -0.78114 Potato 0.6 -1.17171 Potato 0.8 -1.56228 Potato 1.0 -1.95285 The chart above is the data of the osmotic pressure of the solution and the potato core. The osmotic pressure is found using the formula OP=-MiRT. M is the molarity of each cup, which would be the amount of sucrose solution in the cup. For example, Potato 0.4 the molarity would be -0.4. The symbol (i) is the ionization constant that will remain 1 and r is the gas constant, which is 0.0831. This is all multiplied together with the temperature measured in each solution. OP of Potato 0.4 OP=-0.4(1)(0.0831)(23.5) =-0.78114 Data Analysis: The data being shown is the graph of the masses taken in day one and day two of the experiment. The masses were measured and now are analyzed to see if you are able to detect a difference and understand if osmosis was taken place in the sucrose solutions. ...read more.


Conclusion and Evaluation: In conclusion, my hypothesis was correct showing the higher sucrose solution the decrease of mass would occur through osmosis. In the experiment I was able to decipher that the more sucrose involved the more solute the solution gets. When the solution becomes solute it becomes a hypertonic solution that allow the potato cores to get smaller and lets water move out of the cell at higher concentrations. The mass was decreasing in the potatoes because the potatoes were losing water causing the cells to shrivel up. This experiment was very accurate because we used electronic devices to measure all the data points taken. The uncertainties were all put into account so room for error was not a big factor in this experiment. A limitation that can be made is that the experiment was only a two-day period and time wasn't very flexible causing to rush the experiment. Also, it would have been a more decisive experiment if we were able to take more trials of each solution for the best possible accuracy. If I were to make any improvements on the experiment, it would be an increase in days to perform the experiment and also more trials to analyze for accuracy. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Potato IA Lab. This experiment tested osmosis when dealing with different concentrations of ...

    Then 100 mL of the solution was measured in a graduated cylinder and added to the first cup labeled .5 M. This was then repeated for all 5 cups labeled .5 M.

  2. The Effects of Salinity on Wheat Germination

    Day 5 Day 6 Day 7 Day 8 Day 9 Day 10 Day 11 Day 12 Day 13 Day 14 End height 2% number 1 0.0 0.0 0.0 0.0 0.0 0.5 1.0 0.5 0.5 0.5 0.5 1.5 0.5 0.5 6.0 2% number 2 0.0 0.0 0.0 0.0 0.5 1.0 0.5

  1. In this extended essay I am looking at the effect of different kind of ...

    (Yannick Van Doorne) A variety of experiments are a major verification of the fact that music, does in fact affect plant growth. Dorothy Retallack published a small book on this, in 1973, which was based on her experiments of the effect of the music on plant species, in a Colorado College, in Denver.

  2. biology extended essay - How different diets: vegetarian, vegan and a meat centered diet ...

    The participants that switched from one diet to the other stated that they either got sick more often or less often, this is because their body was begining to adapt to the new diet and some side effects may show this however depends from person to person.

  1. Vitamin C Lab

    However, the data still partially corresponds with my hypothesis - as the temperature increases, the concentration of Vitamin C decreases. Conversely, it is interesting that the concentration is lowest when the lemon juice is at 10�C, contradicting with my hypothesis.

  2. The effect on osmosis on the egg shell

    air cell that hampered fully immersion. Although some fluctuations were observed, the general trend (see Graphs 2-3) shows that due to salt concentration of the surrounding increase, the less is change in mass of an egg. According to Graph 1 and Table 5 illustrating mean results all of the measurements,

  1. Experiment to find the water potential of the cytoplasm of potato cell (Solanum tuberosum ...

    * T value > critical value, we reject Ho (null hypothesis) Concentration of sucrose solution/mol dm3 T value> critical value 0.1M and 0.2M 4.4300> 2.78 0.2M and 0.3M 8.6664> 2.78 0.3M and 0.4M 3.5409> 2.78 0.4M and 0.5M 10.6236> 2.78 My results: I rejected Ho for all of the values

  2. Beet Lab. Aim: To determine the effects of temperature on the permeability of ...

    The lower the temperature, the less fluid the membrane becomes and so the less permeable the membrane is. Other things, such as saturated or unsaturated fatty acids in the lipid membrane part also affect fluidity. Beetroot tissue will be used as the model to investigate the types of environmental stresses that affect membrane integrity.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work