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Osmotic Concentration Lab: Abstract (Design)

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Introduction

Title: Osmotic Concentration of Carrot Cells A. Problem - If we take sugar water as an example of a solution, the sugar (sucrose) is called the solute and the water is the solvent. Osmosis is the movement of water across a membrane from an area of lower solute concentration to an area of higher solute concentration. Cells tend to lose water (their solvent) in hypertonic environments (where there are more solutes outside than inside the cell) and gain water in hypotonic environments (where there are fewer solutes outside than inside the cell). When solute concentrations are the same on both sides of the cell, there is no net water movement, and the cell is said to be in an isotonic environment. In this lab we will test samples of carrot tissue to see how much water they absorb or release in sucrose solutions of varying concentrations. This gives us an indirect way to measure the osmotic concentration within living cells, in this case, plant cells. ...read more.

Middle

50 mL beakers 25 mL graduated cylinder Carrots Single edged razor or knife Paper towels Clock or watch 2. Procedure 1) Pre-mix 6 beakers of sucrose solutions in water. 1. Calculate the mass of sucrose necessary to make 25 ml of a solution of 1.0 M sucrose: Necessary info: a. Molar mass of sucrose: 342.3 g/mol b. You want to make 25 ml of solution. c. M = moles/Liter Sample Calculation: a. 1.0 mol/L (1L/1000ml) = 0.001 mol/ml (This step converts from moles/liter to moles/ milliliter) b. 0.001 mol/ml (25 ml) = 0.025 mol (This step allows you to find how many moles of sucrose you need) c. 0.025 mol (342.3 g/mol) = 8.56 g sucrose (This step converts the number of moles to grams that you can mass.) Add your sucrose to a graduated cylinder Add distilled water to the graduated cylinder until the total volume is 25 ml. Pour your solution into a labeled beaker and allow the sucrose to fully dissolve. ...read more.

Conclusion

Be careful not to mix them up. Maybe mass one cup at a time to avoid switching carrot slice with appropriate solution. 8) Determine the final mass and record in Data Table: Osmosis Results 9) Calculate the percent change using the % change equation described in the section entitled Method: Sufficient and Relevant Data Collection. 3. Method: Controlling Variable The dependent variable, percent of change in mass of carrot slice, will be controlled by initially slicing the carrots into relatively similar sizes; if not, the initial mass of the carrots will still be recorded to ensure that I know the original mass of each carrot despite whether or not I sliced them the same size. Furthermore, I will immerse the carrots in the solution for the same amount of time. In addition, I will use the same amount of solution for each of the six carrots. Also, I will dry the carrots before and after immersion to ensure there is no extra weight factored into my calculations. Finally, I will weigh the carrots after immersion to calculate a final mass to utilize in my calculation of percent change in mass. ...read more.

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