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RATE OF REACTIONS OF YEAST AND GLUCOSE, YEAST AND SUCROSE, AND YEAST AND LACTOSE

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Introduction

Aim: The aim of this experiment is to compare the rate of reactions of the reaction of yeast with the three different carbohydrates, namely glucose, sucrose, and lactose. Hypothesis: The rate of reaction should be fastest in the reaction between yeast and sucrose, since sucrose is broken down to two molecules of glucose, thereby giving more glucose for the yeast to act on. Therefore, for the same amount of glucose and sucrose, there will ultimately be double the amount of glucose in sucrose because the yeast will break down the sucrose into two times the amount of glucose. Theory: The three carbohydrates being used in this experiment fall into two categories; monosaccharide and disaccharide. Glucose is a monosaccharide, whereas sucrose and lactose are disaccharides. ...read more.

Middle

Yeast + Glucose ==> Ethanol + Carbon dioxide C6H12O6 ==> 2C2H5OH + 2CO2 Temperature/ �C Number of Carbon Dioxide bubbles released in the reaction in 5 minutes Glucose Lactose Sucrose 20 0 0 0 25 0 0 0 29 0 0 6 35 2 0 54 40 20 0 180 45 100 0 285 52 75 0 215 60 0 0 0 Planning: To carry out this experiment, I have to measure the Data Collection: The data that had to be collected for this experiment was the number of carbon dioxide bubbles released in the reaction in five minutes' time. The rate of there action with different carbohydrates can then be measured and compared. Data Processing and Presentation: From the graph above, it can be observed that the lactose is not digested by yeast and thus no reaction occurs in that test-tube. ...read more.

Conclusion

Some modifications in this experiment could be as follows: * A device that measures and provides the inside temperature of the actual solution should be included in the apparatus. This would provide a clearer picture of the situation. * The rate of release of bubbles is allowed to stabilize first, because the readings can otherwise be misleading. For example, whenever hot water is mixed into the water bath, bubbles are released from the test tube with side arm. These bubbles are the carbon dioxide bubbles from the reaction. They are the consequences of the expansion of air that takes place in the test tubes. Reference: The following resources were used for the experiment and its write-up: * www.google.com * www.accessexcellence.org/AE/AEC/AEF/1995/goodman_respiration.html * " New Understanding Biology for Advanced Level- Fourth Edition" by Glenn and Susan Toole ----------End---------- ...read more.

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