• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Research Question: Which method of DNA extraction (using a centrifuge or not) will yield the most DNA, and which fruit (banana, papaya, or strawberry) contains the most DNA?

Extracts from this document...


DNA Extraction Lab Introduction Research Question: Which method of DNA extraction (using a centrifuge or not) will yield the most DNA, and which fruit (banana, papaya, or strawberry) contains the most DNA? The aim of this investigation is to extract DNA from three organic sources using two methods of extraction in order to determine how much DNA is contained in each source and which method is most effective in extracting DNA. For this investigation, the three sources of DNA are papaya, strawberry, and banana. The difference between the two methods of DNA extraction involves the use of a centrifuge. Hypothesis and Prediction The method involving the centrifuge will yield the most DNA, as it will allow the cell contents of each organic source to separate better than if they were just left to sit. I also predict that the papaya will yield the most DNA, as it appears to be the most genetically advanced. Variables Independent variable: type of fruit used and the use of centrifuge in the extraction process Dependent variable: amount of DNA ...read more.


11. Carefully wind the DNA in the solution around the stirring rod, making sure to extract all of it. 12. Record the mass of the stirring rod, with the DNA. The difference is the amount of DNA extracted. 13. Repeat the procedure five more times, being sure to use one sample of each fruit without the centrifuge and one sample of each with the centrifuge. Data: Extraction Without Centrifuge Fruit Type Mass of Stirring Rod (grams) Mass of Stirring Rod with DNA (grams) Amount of DNA extracted (first mass subtracted from second mass) (grams) Banana 52.1 57.5 4.3 Papaya 50.8 58.9 4.7 Strawberry 54.9 50.2 3.9 Average 4.433 grams Extraction With Centrifuge Fruit type Mass of Stirring Rod (grams) Mass of Rod with DNA (grams) Amount of DNA extracted (grams) Banana 55.1 58.3 6.4 Papaya 58.3 50.9 7.0 Strawberry 58.4 59.3 6.2 Average 6.533 grams Calculations To determine amount of DNA extracted, I subtracted the mass of the stirring rod from the mass of the stirring rod with the DNA. ...read more.


Statement of improvement: This lab could be improved by using a better filtering system. The coffee filters left too much liquid with the solid it strained. Perhaps a stronger filter would be better, as the remaining solid could be pressed and yield more liquid. The DNA invariably contained fluid when it was extracted, so drying the pure DNA before recording its mass would remove that extraneous mass. Utilizing a blender or another more efficient method of pulverizing the fruit would yield more DNA, as it would break all cells. Learning Statement: What I learned from this experiment was that the inclusion of one additional step (with the centrifuge) could have a profound effect on the amount of DNA yielded. I learned that the centrifuge separates the contents of a solution far more effectively than letting the solution sit. I also learned that polyploidy plays a role in the amount of DNA contained in each fruit, as the fruit with the most chromosomes yield the most DNA. This experiment shows the importance designing an experiment with the right procedures, as the omission of one step produced a much lower percent yield of DNA. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Neurology and Behaviour. Focus question: Is there an increase in the perception and ...

    5 star(s)

    have meant to give an image a score of five however felt that this rating may be considered too high and as a results rerated it. The results were entirely based on the individuals rating of the images and as such vary in uncertainty and reliability.

  2. DNA Strawberry Extraction Lap

    What is the purpose for the shampoo and salt in the DNA extraction buffer? The detergent in the shampoo dissolves the phospholipids bi-layers of the cell and organelle membranes. The positive charged sodium ions in the extraction solution are attracted to the negatively charged phosphate groups on the DNA backbone.

  1. Independent Research Project Vital Lung Capacity

    vital capacity exhaled by participants whom suffer from asthma and those whom do not. Specifically, there is only a difference of 0.52 liters. This isn't a big difference in but nonetheless it does support the original experimenter's hypothesis. Of course the reason why there is in fact this difference between

  2. DNA Extraction Lab

    Ethanol is added to precipitate the DNA, since DNA is soluble in water, so when ethanol is added the DNA will precipitate into the alcohol layer.

  1. The Use of Recombinant DNA Technology

    which means then that actually the whole sequence is cut out of the DNA coding for a protein. In terms recombinant DNA enzymes are used, which cut the DNA to have sticky ends. This means that the end is cut in a staggered fashion to allow the matching fragments to be rejoined.

  2. Genes of fruit fly practical write up

    sex dependant is also very similar to yellow and sable body colour. There were no females for the recessive tan coloured body. This could also be due to a disease that affects females of the recessive trait. The ratio is 3:1 as the wild type is dominant and the tan coloured body recessive.

  1. Biology independent investigation, Ecology

    One of the most reliable ways to measure the development of a plant is to measure is dry weight. This means that water will not be a contributing factor to the weight and the only factor will be the actually plant mass and therefore the amount of growth.

  2. Effect of Detergent on Membrane Permeability

    bleed in environment with high temperature, which will make the experiment unfair because 2 variables are changed. Due to the viscosity of the detergent, it was also hard to measure the amount of solution. We also do not know how the percentages of detergent in the solutions are measured.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work