• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Research Question: Which method of DNA extraction (using a centrifuge or not) will yield the most DNA, and which fruit (banana, papaya, or strawberry) contains the most DNA?

Extracts from this document...

Introduction

DNA Extraction Lab Introduction Research Question: Which method of DNA extraction (using a centrifuge or not) will yield the most DNA, and which fruit (banana, papaya, or strawberry) contains the most DNA? The aim of this investigation is to extract DNA from three organic sources using two methods of extraction in order to determine how much DNA is contained in each source and which method is most effective in extracting DNA. For this investigation, the three sources of DNA are papaya, strawberry, and banana. The difference between the two methods of DNA extraction involves the use of a centrifuge. Hypothesis and Prediction The method involving the centrifuge will yield the most DNA, as it will allow the cell contents of each organic source to separate better than if they were just left to sit. I also predict that the papaya will yield the most DNA, as it appears to be the most genetically advanced. Variables Independent variable: type of fruit used and the use of centrifuge in the extraction process Dependent variable: amount of DNA ...read more.

Middle

11. Carefully wind the DNA in the solution around the stirring rod, making sure to extract all of it. 12. Record the mass of the stirring rod, with the DNA. The difference is the amount of DNA extracted. 13. Repeat the procedure five more times, being sure to use one sample of each fruit without the centrifuge and one sample of each with the centrifuge. Data: Extraction Without Centrifuge Fruit Type Mass of Stirring Rod (grams) Mass of Stirring Rod with DNA (grams) Amount of DNA extracted (first mass subtracted from second mass) (grams) Banana 52.1 57.5 4.3 Papaya 50.8 58.9 4.7 Strawberry 54.9 50.2 3.9 Average 4.433 grams Extraction With Centrifuge Fruit type Mass of Stirring Rod (grams) Mass of Rod with DNA (grams) Amount of DNA extracted (grams) Banana 55.1 58.3 6.4 Papaya 58.3 50.9 7.0 Strawberry 58.4 59.3 6.2 Average 6.533 grams Calculations To determine amount of DNA extracted, I subtracted the mass of the stirring rod from the mass of the stirring rod with the DNA. ...read more.

Conclusion

Statement of improvement: This lab could be improved by using a better filtering system. The coffee filters left too much liquid with the solid it strained. Perhaps a stronger filter would be better, as the remaining solid could be pressed and yield more liquid. The DNA invariably contained fluid when it was extracted, so drying the pure DNA before recording its mass would remove that extraneous mass. Utilizing a blender or another more efficient method of pulverizing the fruit would yield more DNA, as it would break all cells. Learning Statement: What I learned from this experiment was that the inclusion of one additional step (with the centrifuge) could have a profound effect on the amount of DNA yielded. I learned that the centrifuge separates the contents of a solution far more effectively than letting the solution sit. I also learned that polyploidy plays a role in the amount of DNA contained in each fruit, as the fruit with the most chromosomes yield the most DNA. This experiment shows the importance designing an experiment with the right procedures, as the omission of one step produced a much lower percent yield of DNA. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Neurology and Behaviour. Focus question: Is there an increase in the perception and ...

    5 star(s)

    It is possible to suggest that this may be dues to the genetic need to protect female survival for the continuation of the species or merely due to the way in which girls are raised to be more clean in nature.

  2. DNA Strawberry Extraction Lap

    What is the purpose for the shampoo and salt in the DNA extraction buffer? The detergent in the shampoo dissolves the phospholipids bi-layers of the cell and organelle membranes. The positive charged sodium ions in the extraction solution are attracted to the negatively charged phosphate groups on the DNA backbone.

  1. Independent Research Project Vital Lung Capacity

    5 1 3.2 3.3 2 3.2 3 3.3 Total Average: 3.28 Table 2: Each participant was asked to give 3 trails; hence on this table all three are shown. The maximum trial was used to calculate the total average of non-asthmatic contributors.

  2. Research Project . Should Research Into Biological Warfare Continue?

    The only known example of human inhalational Botulin occurred when three German laboratory scientists were accidentally exposed during an animal experiment. Other known trials of Botulin occurred in the early 1990's by a Japanese cult called Aum Shinrikyo which attempted to spread it as an aerosol 3 times; and in

  1. Genes of fruit fly practical write up

    sex dependant is also very similar to yellow and sable body colour. There were no females for the recessive tan coloured body. This could also be due to a disease that affects females of the recessive trait. The ratio is 3:1 as the wild type is dominant and the tan coloured body recessive.

  2. Bacteria and Cleanliness. Question: Among the insides of a toilet, dishcloth, sink, and a ...

    I do not have to worry about the inoculating of bacteria since I will be using an incubator, and that assures me that all the conditions in the incubator will be kept consistent for 36 hours. Data Collection: Data collection will be collected by using an automatic cell counter that counts the bacteria in colonies.

  1. Effect of Detergent on Membrane Permeability

    causes the detergent to have less absorption rate and become more transparent. This is unlikely to be a systematic error because the results from all trials and every set of beet lead to the same result. Since the amount of change in absorbance increases as the concentration increases (graph3), and

  2. The Story of the Discovery of DNA

    Rosalind Franklin produced several, remarkable x-ray photographs, nonetheless even she did not realize how her photographs showed that DNA was a double helix. It took the initiative of the Watson-Crick team to make this evident. Before Crick saw the x-ray photographs, he heard about them from Watson.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work