• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Sewage design lab

Extracts from this document...


Kaleem Atchia February 15th, 2009 SBI3U7-AA (Grade 11 IB Biology) Mr. Pourkhatai Sewage Treatment Design Lab Disinfectants and E. coli Research Question: How can you determine which method of disinfection is most effective in eliminating E. coli within water? Introduction: At the tertiary treatment of sewage, nitrogen and phosphorus compounds are removed but the water is also disinfected to kill all micro-organisms to make sure no pathogens are released in nature. In this experiment, a harmless strain of E. coli (HB 101) will simulate the presence of bacteria in water. Different methods of disinfection such as boiling, UV light exposure and chlorination will be used. The physical methods which are primary disinfectants only, will be compared to each other. Likewise the chemical methods which also have a residual effect will be compared to each other. After treatment, a Colilert testing kit will be used which would detect the presence of coliforms in water by a colour change to yellow. ...read more.


2. The approximate number of E. coli bacteria will be somewhat controlled. 2 mL of the E. coli culture will be placed in 10 mL of water. 3. The volume of solutions added for the chemical methods will be constant. 2 mL of the solutions will be added to the appropriate trials. 4. The time the chemicals are allowed to act unaffected will be 5 minutes for each trial. After that time, the Colilert reagent will be added. 5. The testing for bacteria will be the same for all trials. 10 mL of the Colilert reagent will be added to each trial. 6. The time allowed for Colilert test to act in incubation. 24 hours, same for all trials. Materials: * Beakers * Graduated cylinder * Pipets * Retort stand * Ring clamp * Thermometer * Stopwatch * Heating plate * Waterproof marker * Spectrophotometer * Distilled water * E. coli culture * Incubator * Colilert Test kit * Sodium hypochlorite * Calcium hypochlorite * UV lamp Procedure: 1. ...read more.


Heat the water until it boils, check temperature with thermometer. Set retort stand and ring clamp to hold the smaller beaker. 8. Repeat step 3 and place the beaker in the clamp 9. Lower the beaker with the clamp into the bath and time 30 seconds with the stopwatch before removing it and adding 10 mL of Colilert reagent. Label the beaker accordingly and place it in the incubator. 10. Repeat steps 8 and 9 but time 2 minute and 8 minutes for each trial. 11. Repeat step 3 and place it on the table. 12. Shine the UV lamp upon it for 15 seconds. Then add 10 mL of Colilert reagent before labelling it and placing it in the incubator. 13. Repeat step 11 and 12 but time 2 minute and 8 minutes for each trial. 14. Come back in 24 hours. Remove the control and place a sample through the spectrophotometer, setting the wavelength as the baseline value. 15. Take a sample of each trial and place it within the spectrophotometer, recording the readings carefully. ?? ?? ?? ?? Page 1 of 2 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Ecology Design IA

    pH meter attachment to Xplorer GLX - Thermometer METHOD Prior to recording of data (Approx. 5:30am, however must be before sunrise) 1. The area within the rocky shore in the lower intertidal zone was determined and the 1 x 1m quadrat was placed on the area to be studied 2.

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    This means that germinating seeds consumer more oxygen than the non-germinating seeds in both room temperature and ice water. The reason why germinating seeds consumes more oxygen is that they process faster cellular respiration. The faster cellular respiration occurs, the more oxygen it consumes.

  1. Biology Extended Essay 2009

    Experiment- Comparing Vitamin C content As the three types of orange juice are different in characteristics and the method of processing, a simple laboratory experiment is constructed to investigate and compare the content of vitamin C to further explore and pursue the purpose of the research.

  2. Protein Purification Lab

    +/-0.5g Weight of protein in beaker #5 (g) +/-0.5g 4:20 10.0 10.0 10.0 10.0 10.0 4:50 40.6 67.2 93.8 125 151 5:20 37.8 61.6 84.1 118 140 5:50 33.6 56.1 84.0 113 140 Data presentation: Steps to calculate the protein digestion rate in one time period: 100% - (Mass (g)

  1. Experimental Design Notes

    the middle and thin outwards o Discovered that holding lens over an object made it larger o Magnifying glass o 1590, 2 Dutch spectacle makers, Zaccharias and Hans Janssen put more then one lens together and looked through saw object larger o More novelty than function o Anthony Van Leeuwenhoek

  2. Biology Industrial Melanism of Peppered Moth Lab

    Number of Melanic Moths and Light-Coloured Moths left on Dark- Coloured "Tree Bark" Trial Number Number of Melanic Moths Number of Light-Coloured Moths 1 19 11 2 15 15 3 18 12 4 25 5 5 26 4 Table 13.

  1. Invertebrate Biodiversity Lab

    They lack a vertebral column. These invertebrates are worms or insects. Air, earth, energy from the sun and all living organisms found on Earth depends on one another. Therefore abiotic factors such as soil, temperature and water; as well as biotic factors- the types of trees and other living organisms

  2. Identification of Organic Compounds

    Variable: The independent variable was varied by using different known solutions with the Benedict's reagent to create a variety of results. * Dependent (Responding) Variable: The dependent variable was monitored by recording the color change of the result between the reactions of the solutions with the Benedict's reagent.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work