Sleep is a normal part of human life. Investigate the neurobiological basis of normal healthy sleep and sleep-deprivation.

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Cherno Okafor

Aida Stefani

SBI4U

December 29th, 2012

Independent Research Project

Topic: Sleep is a normal part of human life. Investigate the neurobiological basis of normal healthy sleep and sleep-deprivation.

Part 1: Abstracts (Summarize your findings)

My report/issue is about comparing normal, healthy sleep and sleep deprivation among infants, adults, and the elderly by analyzing the neurology and physiology of sleep and the effects on the human body.

First I will define what sleep is and explain its significance. Sleep is a behavioural state that is a natural part of every individual’s life. We spend about one-third of our lives asleep. Nonetheless, people generally know little about the importance of this essential activity. Sleep is not just something to fill time when a person is inactive. Sleep is a required activity, not an option. Even though the precise functions of sleep remain a mystery, sleep is important for normal motor and cognitive function. We all recognize and feel the need to sleep. After sleeping, we recognize changes that have occurred, as we feel rested and more alert. Sleep actually appears to be required for survival. Moreover, sleep is a naturally recurring state characterized by reduced or absent consciousness, relatively suspended sensory activity, and inactivity of nearly all voluntary muscles. It is distinguished from quiet wakefulness by a decreased ability to react with stimuli, and is more easily reversible than being in hibernation or a coma. Sleep is a heightened anabolic state, accentuating the growth and rejuvenation of the immune, nervous, skeletal and muscular systems. It is observed in all mammals, birds, reptiles, amphibians, and fish.

The purposes and mechanisms of sleep are only partially clear and are the subject of intense research. Sleep is often thought to help conserve energy, but decreases metabolism only about 5-10%

Normal Sleep in Adults, Infants, and the Elderly:

Normal sleep is divided into non-rapid eye movement (NREM) and rapid eye movement (REM) sleep. NREM sleep if further divided into progressively deeper stages of sleep: stage N1, stage N2, and stage N3 (deep or delta-wave sleep). As NREM stages progress, stronger stimuli are required to result in an awakening. Stage R sleep (REM sleep) has tonic and phasic components. The phasic component is a sympathetically driven state characterized by rapid eye movements (rolling eyes, opening and closing), muscle twitches, and respiratory variability. Tonic REM is a parasympathetically driven state with no eye movements. The REM period length and density of eye movements increases throughout the sleep cycle.

Waking usually transitions into light NREM sleep. NREM sleep typically begins in the lighter stages of N1 and N2, and progressively deepens to slow wave sleep as evidenced by higher-voltage delta waves. N3 (slow wave sleep) is present when delta waves account for more than 20% of the sleep EEG. REM sleep follows NREM sleep and occurs 4-5 times during a normal 8-hour sleep period. The first REM period of the night may be less than 10 minutes in duration, while the last may exceed 60 minutes. The NREM-REM cycles vary in length from 70-100 minutes initially to 90-120 minutes later in the night. The N3 stage of NREM has implications of parasomnias such as: night terrors, sleepwalking, etc.

Typically, N3 sleep is present more in the first third of the night, whereas REM sleep predominates in the last third of the night with the presence of N3 sleep. This contrasts with REM sleep behaviour disorder (RBD), which typically occurs in the last half of the night.

Sleep in adults: Stage N1 is considered a transition between wake and sleep. It occurs upon falling asleep and during brief arousal periods within sleep and usually accounts for 2-5% of total sleep time. Stage N2 occurs throughout the sleep period and represents 45-55% of total sleep time. Stage N3 (delta or slow wave sleep) occurs mostly in the first third of the night and constitutes 5-15% of total sleep time. REM represents 20-25% of total sleep time and occurs in 4-5 episodes throughout the night.

Sleep in infants: Infants have an overall greater total sleep time than any other age group; their sleep time can be divided into multiple periods. In newborns, the total sleep duration in a day can be 14-16 hours. Over the first several months of life, sleep time decreases; by age 5-6 months, sleep consolidates into an overnight period with at least 1 nap during the day. REM sleep in infants represents a larger percentage of the total sleep at the expense of stage N3. Until age 2-4 months, newborns transition from wake into REM sleep. Thereafter, wake begins to transition directly into NREM.

Overall, electrocortical recorded voltage remains high during sleep, as it does during period of wakefulness. Sleep spindles begin appearing in the second month of life with a density greater than that seen in adults. After the first year, the spindles begin decreasing in density and progress toward adult patterns. K complexes develop by the sixth month of life.

Sleep in the elderly: In elderly persons, the time spent in stage N3 sleep decreases, and the time in stage N2 increases to compensate. Latency  to fall asleep and the number and duration of overnight arousal periods increase This often causes total time in bed to increase which can lead to complaints of insomnia. Sleep fragmentation results from the increase in overnight arousals and may be aggravated by the increasing number of medical conditions, including sleep apnea, musculoskeletal disorders, and cardiopulmonary disease.

Sleep Physiology:

Sleep is a state of unconsciousness in which the brain is relatively more responsive to internal than external stimuli. The predictable cycling of sleep and the reversal of relative external unresponsiveness are features that assist in distinguishing sleep from other states of unconsciousness. The brain gradually becomes less responsive to visual, auditory, and other environmental stimuli during the transition from wake to sleep, which is considered by some to be stage 1 of sleep.  

Historically, sleep was thought to be a passive state that was initiated through withdrawal of sensory input. Currently, withdrawal of sensory awareness is believed to be a factor in sleep, but an active initiation mechanism that facilitates brain withdrawal is also recognized. Both homeostatic factors (factor S) and circadian factors (factor C) interact to determine the timing and quality of sleep.

The “switch” for sleep is considered to be the ventrolaterla preoptic nucleus (VLPO) of the anterior hypothalamus. This area becomes active during sleep and uses the inhibitory neurotransmitters GABA and galanin to initiate sleep by inhibiting the arousal regions of the brain. The VLPO innervates and can inhibit the wake-promoting regions of the brain.

NREM is an active state that is maintained partly through oscillations between the thalamus and the cortex. The 3 major oscillation systems are sleep spindles, delta oscillations, and slow cortical oscillations. Sleep spindles of stage N2 sleep, are generated by bursts of hyperpolarizing GABAnergic neurons in the nucleus of the thalamus. Delta waves are produced by interactions from both thalamic reticular and cortical pyramidal sources. Slow cortical oscillations are produced in neocortical networks by cyclic hyperpolarizations and depolarizations.

Some theories of NREM are that there is a decreased metabolic demand which facilitates replenishment of glycogen stores. Another theory suggests that the oscillating depolarizations and hyperpolarizations consolidate memory and remove redundant or excess synapses.

Hypnogram showing sleep cycles from midnight to 6:30am, with deep sleep early on. There is more REM (marked red) before waking.

Circadian Rhythms that influence Sleep

Sleep timing is controlled by the circadian clock, sleep-wake homeostasis, and in humans, within certain bounds, willed behaviour. The circadian clock-an inner timekeeping, temperature-fluctuating, enzyme-controlling device-works in tandem with adenosine, a neurotransmitter that inhibits many of the bodily processes associated with wakefulness. Adenosine is created over the course of the day; high levels of adenosine lead to sleepiness. Circadian sleep rhythm is one of the several intrinsic body rhythms regulated by the hypothalamus. The “suprachiasmatic” nucleus sets the body clock to approximately 24.2 hours, with both light exposure and schedule clues entraining to the 24.2 hour cycle. The “retinohypothalamic” tract allows light cues to directly influence the “suprachiasmatic” nucleus. Light is called a zeitgeber, a German word meaning time-giver, because it sets the suprachiasmatic clock. A practical purpose has been proposed for the circadian rhythm, using the analogy of the brain being somewhat like a battery charging during sleep and discharging during the wake period.

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Homeostatic sleep propensity (the need for sleep as a function of the amount of time elapsed since the last adequate sleep episode) must be balanced against the circadian element for satisfactory sleep. Along with corresponding messages from the circadian clock, this tells the body it needs to sleep. Sleep offset (awakening) is primarily determined by circadian rhythm. A person who regularly awakens at an early hour will generally not be able to sleep much later than his or her normal waking time, even if they were moderately sleep-deprived. Sleep duration is affected by the gene DEC2. Some people have a ...

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