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sucrose concentration affects potato cells

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Introduction

How do solutions of various concentrations affect potato cells? Aim: Do the masses of potato cells change when affected with different concentrations of sucrose? Hypothesis: The lower the concentration of sucrose, the higher the mass of the potato piece. Justification: The more dilute a sucrose solution is, the more water there is. Since osmosis is the movement of water from high water concentration to low water concentration through a partially permeable membrane, it is expected that the higher concentration of water in sucrose with low molarity will increase the mass of potato cells. Therefore, a hypotonic sucrose solution (low molarity) will increase the mass of potato pieces as potato cells will become turgid as water enter the cells and hypertonic sucrose solutions will decrease the mass of potato pieces as water will move outside of the potato cells to a less water-concentrated solution. Variables Independent: Concentration/molarity of sucrose - 0.00M (water only), 0.25M, 0.50M, 0.75M, 1.00M (sucrose only Dependent: The mass of potato pieces Controlled: * Size of potato pieces (5.4�0.5mm) * Temperature of solution and surrounding temperature * Volume of solutions (20cm�) ...read more.

Middle

(g) Trial 2 (Final mass - Initial mass) (g) Trial 3 (Final mass - initial mass) (g) 0.00M (water only) 0.02 0.07 0.06 0.25M -0.01 -0.01 -0.17 0.5M -0.1 -0.05 -0.08 0.75M -0.13 -0.11 -0.14 1.0M (sucrose only) -0.16 -0.17 -0.15 Percentage change of the initial and final mass of the potato pieces Molarity of the potato pieces immersed in Trial 1 (mass difference � initial mass � 100) (%) Trial 2 (%) Trial 3 (%) Average percentage change (%) 0.00M 4.17 10 8.45 7.54 0.25M -1.61 -1.64 -23.94 -9.06 0.50M -18.18 -6.5 -12.5 -12.4 0.75M -16.46 -22 -20.59 -19.68 1.00M -29.63 -17.71 -22.06 -23.13 Discussion The graph of the percentage change of the mass of the potato pieces shows a negative correlation. It shows that the percentage change of the mass of the potato pieces decreases as the concentration of sucrose increase. This also means that the mass of the potato pieces increase and then decreased as the concentration of sucrose increased. The results of this experiment suggests that through osmosis, water entered the potato cells in lower concentration of sucrose and water left the potato cells in higher concentrations of sucrose. ...read more.

Conclusion

That way, we will have a more accurate graph and we will get a more accurate estimation for the isotonic point. Since we had to consider the amount of time we were given, we could only do 3 trials More accurate results could be obtained if we worked on more than 3 trials. For example, 5 trials. The more trials we have, the more valid the average will be. This could also help defeat any anomalies in the raw data. One of the uncertainties that have been identified is the measurement of water and sucrose. To defeat the uncertainty, it more accurate to use a longer and thinner measuring cylinder than the 100cm� that was used. If possible it could be more accurate to use a burette or a pipette. Conclusion The experiment that I have conducted has given me valid results. After calculating the results and graphing the percentage change of the mass of the potato pieces, my hypothesis was proven to be correct. From the negative correlation that my graph has shown, I can confirm that as the concentration of sucrose increase, the mass of the potato pieces will decrease. ?? ?? ?? ?? Syazana Asmuni 12 b ...read more.

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