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The Effect of Alcohol on Biological Membranes

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The Effect of Alcohol on Biological Membranes ________________ Procedure 1. Obtain and wear goggles, an apron, and gloves. 2. Obtain the following materials: a. Place about 10 mL of methanol in a medium sized test tube. Label this tube M. b. Place about 10 mL of ethanol in a medium sized test tube. Label this tube E. c. Place about 10 mL of 1-propanol in a medium sized test tube. Label this tube P. d. Place about 30 mL of tap water in a small beaker. 3. Prepare five methanol solutions (0%, 10%, 20%, 30% and 40%). Using Beral pipets, add the number of drops of water specified in Table 1 to each of five wells. Use a different Beral pipet to add alcohol to each of five wells in the microwell plate. See Table 1 to determine the number of drops of alcohol to add to each well. 4. Clean the pipet used to transfer alcohol. To do this, wipe the outside clean and empty it of liquid. Draw up a little ethanol into the pipette and use the liquid to rinse the inside of the pipette. Discard the ethanol. 5. Prepare five ethanol solutions. To do so, repeat Step 3, substituting ethanol for methanol. ...read more.


0.195 0.197 0.202 0.166 0.501 0.177 0.230 0.333 0.157 0.131 0.226 0.402 0.849 0.144 0.086 0.181 0.173 1.34 0.158 0.200 0.202 0.141 0.318 0.218 0.191 0.184 0.165 0.920 0.181 0.256 0.208 0.154 0.987 1.09 0.169 0.185 0.222 0.266 0.936 0.169 0.172 0.191 0.176 0.156 0.228 0.171 0.171 0.326 0.199 0.135 0.241 0.229 0.283 0.473 0.182 0.178 0.200 0.621 0.172 0.207 0.158 0.172 0.445 0.167 0.228 0.197 0.216 0.679 0.221 0.188 0.211 0.194 0.200 0.126 0.194 Table 2: Table showing raw data of propanol absorbance Concentration of Ethanol Light Absorbance(± 0.001 AU) 0% 10% 20% 30% 40% 0.202 0.257 0.366 0.163 1.23 0.309 0.191 0.287 0.171 0.145 0.298 0.256 0.202 0.196 0.159 0.350 0.225 0.227 0.23 0.166 0.245 0.283 0.25 0.242 0.147 0.233 0.173 0.171 0.218 0.206 0.232 0.280 0.223 0.172 0.161 0.278 0.233 0.261 0.188 0.196 0.276 0.197 0.229 0.178 0.186 0.271 0.178 0.224 0.146 0.13 0.262 0.184 0.205 0.196 0.36 0.289 0.207 0.241 0.157 0.18 0.279 0.202 0.215 0.285 0.107 0.306 0.184 0.242 0.231 0.221 0.277 0.126 0.14 0.24 Table 3: Table showing raw data of ethanol absorbance Processed data Concentration of alcohol (±1%) Methanol Propanol Ethanol 0% 0.264 0.192 0.274 10% 0.267 0.204 0.212 20% 0.254 0.53 0.239 30% 0.222 0.304 0.184 40% 0.48 0.204 0.256 Table 4: Table showing average light absorption for each alcohol solution Figure 1: Graph showing average light ...read more.


Furthermore, it is possible that the beetroot cubes were not thoroughly rinsed to rid them of the betacyanin pigment that was released during the cutting. This would give the solution in the well a higher level of pigment absorbance regardless of the damage done by the alcohol in the solution. Step 9, which requires stirring the beet in the alcohol solution, cautions us to not puncture the beetroot during stirring. If the beetroot was punctured it would also release pigment into the solution that was not caused by alcohol damage to the membranes. My lab partner and I were responsible for finding the data for the methanol alcohol. We pooled our data with the other groups to obtain the information for the other two alcohols. It is impossible for me to know what errors were made in the other two groups? data collection as I was not a part of it. The investigation could be improved if the experimenters were extremely vigilant during the setup process, especially any steps involving the handling of the beetroot to ensure it is not damaged and doesn?t negatively impact the results. Using more types of alcohol would give us a more precise representation of the effect of alcohol on the biological membrane. It is also important to ensure that the Colorimeter is calibrated properly to ensure precise results are given. ________________ [1] Polarity vs. Chain Lenglths in Organic Compounds. Virtual Chembook Elmhurst College. Web. 03.04.12. www.elmhurst.edu/~chm/vchembook/214organicchain.html ...read more.

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