• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Alcohol on Biological Membranes

Extracts from this document...

Introduction

´╗┐The Effect of Alcohol on Biological Membranes ________________ Procedure 1. Obtain and wear goggles, an apron, and gloves. 2. Obtain the following materials: a. Place about 10 mL of methanol in a medium sized test tube. Label this tube M. b. Place about 10 mL of ethanol in a medium sized test tube. Label this tube E. c. Place about 10 mL of 1-propanol in a medium sized test tube. Label this tube P. d. Place about 30 mL of tap water in a small beaker. 3. Prepare five methanol solutions (0%, 10%, 20%, 30% and 40%). Using Beral pipets, add the number of drops of water specified in Table 1 to each of five wells. Use a different Beral pipet to add alcohol to each of five wells in the microwell plate. See Table 1 to determine the number of drops of alcohol to add to each well. 4. Clean the pipet used to transfer alcohol. To do this, wipe the outside clean and empty it of liquid. Draw up a little ethanol into the pipette and use the liquid to rinse the inside of the pipette. Discard the ethanol. 5. Prepare five ethanol solutions. To do so, repeat Step 3, substituting ethanol for methanol. ...read more.

Middle

0.195 0.197 0.202 0.166 0.501 0.177 0.230 0.333 0.157 0.131 0.226 0.402 0.849 0.144 0.086 0.181 0.173 1.34 0.158 0.200 0.202 0.141 0.318 0.218 0.191 0.184 0.165 0.920 0.181 0.256 0.208 0.154 0.987 1.09 0.169 0.185 0.222 0.266 0.936 0.169 0.172 0.191 0.176 0.156 0.228 0.171 0.171 0.326 0.199 0.135 0.241 0.229 0.283 0.473 0.182 0.178 0.200 0.621 0.172 0.207 0.158 0.172 0.445 0.167 0.228 0.197 0.216 0.679 0.221 0.188 0.211 0.194 0.200 0.126 0.194 Table 2: Table showing raw data of propanol absorbance Concentration of Ethanol Light Absorbance(± 0.001 AU) 0% 10% 20% 30% 40% 0.202 0.257 0.366 0.163 1.23 0.309 0.191 0.287 0.171 0.145 0.298 0.256 0.202 0.196 0.159 0.350 0.225 0.227 0.23 0.166 0.245 0.283 0.25 0.242 0.147 0.233 0.173 0.171 0.218 0.206 0.232 0.280 0.223 0.172 0.161 0.278 0.233 0.261 0.188 0.196 0.276 0.197 0.229 0.178 0.186 0.271 0.178 0.224 0.146 0.13 0.262 0.184 0.205 0.196 0.36 0.289 0.207 0.241 0.157 0.18 0.279 0.202 0.215 0.285 0.107 0.306 0.184 0.242 0.231 0.221 0.277 0.126 0.14 0.24 Table 3: Table showing raw data of ethanol absorbance Processed data Concentration of alcohol (±1%) Methanol Propanol Ethanol 0% 0.264 0.192 0.274 10% 0.267 0.204 0.212 20% 0.254 0.53 0.239 30% 0.222 0.304 0.184 40% 0.48 0.204 0.256 Table 4: Table showing average light absorption for each alcohol solution Figure 1: Graph showing average light ...read more.

Conclusion

Furthermore, it is possible that the beetroot cubes were not thoroughly rinsed to rid them of the betacyanin pigment that was released during the cutting. This would give the solution in the well a higher level of pigment absorbance regardless of the damage done by the alcohol in the solution. Step 9, which requires stirring the beet in the alcohol solution, cautions us to not puncture the beetroot during stirring. If the beetroot was punctured it would also release pigment into the solution that was not caused by alcohol damage to the membranes. My lab partner and I were responsible for finding the data for the methanol alcohol. We pooled our data with the other groups to obtain the information for the other two alcohols. It is impossible for me to know what errors were made in the other two groups? data collection as I was not a part of it. The investigation could be improved if the experimenters were extremely vigilant during the setup process, especially any steps involving the handling of the beetroot to ensure it is not damaged and doesn?t negatively impact the results. Using more types of alcohol would give us a more precise representation of the effect of alcohol on the biological membrane. It is also important to ensure that the Colorimeter is calibrated properly to ensure precise results are given. ________________ [1] Polarity vs. Chain Lenglths in Organic Compounds. Virtual Chembook Elmhurst College. Web. 03.04.12. www.elmhurst.edu/~chm/vchembook/214organicchain.html ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. The aim of the investigation is to find out how an increase in surrounding ...

    they could have been and there are a number of factors that could contribute to my level of accuracy and the results that I got. The fact that my results follow a genuine trend suggests that my data was not far off however compared to some of the other students,

  2. The effect on osmosis on the egg shell

    According to results recorded after second submergence the range equaled to 5.41, and after third immersion, the range in measurements was 5.7. In set 2 and 3 results did not deviate so much. What more can be said about statistical analysis is that there was no mode, median values were

  1. In this extended essay I am looking at the effect of different kind of ...

    Remember to bury those 4 cm in depth from the top of the soil. Also remember to prepare soil as mentioned in variable above. Observations: The radicle of the seed has grown a little longer in length on each category of plant.

  2. The effect of pvc piping on the breathing/heart rates of male year 12 students

    Additionally generally healthy subjects will be chosen. 1.3.3- Method Heart rate 1-Subject is asked to sit down and breathe normally 2- Time subject for 30 seconds 3- After 30 second heart rate is taken for 1 minute 4-Recond number of heart beats after 1 minute 5- Subject is asked to

  1. Beetroot Plasma Investigation. The main objective of this investigation is to focus on ...

    * Time beetroot slices are in solution- This is vital because the time in which they are in the solution is the time by which the red pigment will diffuse out of the disc and dissolve in the solution. Therefore this will have to be kept the same so that the time given for each is fair and equal.

  2. Allelopathy. Open Investigation Will increasing the number of allelopathic sunflower plants effect the ...

    working in a circular motion from the edges of the pot towards the middle of the pot 13. Place the pots at an undisturbed area where there is presence full sunlight in the day Daily care 1. Every day at 4:00pm, use the measuring cup to water each pot with 500mL of rain water.

  1. Beet Lab. Aim: To determine the effects of temperature on the permeability of ...

    Before starting the experiment, we should just wipe the apparatus to ensure zero error. 7. Do not overtime the experiment. 8. When the beetroot was cut some of the membrane was broken causing the pigment to leak out this must be thoroughly cleaned and washed off to secure the accuracy of results.

  2. Effect of Detergent on Membrane Permeability

    If there are more detergent molecules available in the water, more phospholipid heads will be repelled, thus more cell membrane would be penetrated and more betalaine will be able to leak out of the cell exponentially. However, as depicted by the graphs, the betacyanin leaked from the beet cell somehow

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work