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THE EFFECT OF TEMPERATURE ON THE MOVEMENT OF PIGMENT THROUGH THE CELL MEMBRANES ________________ Table showing the effect of different temperature levels in degrees Celsius on the movement of the red anthocyanin pigment from the beetroot cell to distilled water after 30 minutes, using a colorimeter to measure solute concentration as determined by % transmission Temperature in degrees Celsius. ±1°c % transmission of light through solution in the cuvette. ±1% Trial 1 Trial 2 Trial 3 Trial 4 Trial 5 % transmission mean (1 decimal place )±1% Standard deviation(%) (1 decimal place) 85 56 48 52 62 54 54.4 5.2 80 69 58 54 64 73 63.6 7.8 75 78 66 56 67 78 69.0 9.3 70 85 71 68 72 83 75.8 7.7 65 88 80 82 82 93 85.0 5.4 63 92 88 90 84 98 90.4 5.2 60 94 92 90 96 98 94.0 3.2 55 96 94 92 98 99 95.8 2.9 50 96 98 98 99 99 98.0 1.2 45 98 99 100 100 100 99.4 0.9 ________________ Trial 1 was conducted by me. ...read more.


It also consists of integral proteins which could act as enzymes,as the temperature rises to 85°c,they were denatured thus creating holes for the anthocyanin to diffuse easily. Therefore, to a certain extent, temperature has an effect on the movement of pigment through the cell membranes. The higher the temperature,the more integral proteins are denatured,as a result more holes are created thus speeding up diffusion. EVALUATION WEAKNESSES SOLUTION 1.the beetroot roots were not precisely of the same size. This could have affected the rate of diffusion and the amount of anthocyanin released in the distilled water. If one of the roots were larger, there would be a higher area for diffusion,thus speeding it up and more pigment would be released as it would have more cells and vacuoles containing the pigment. This would defy the rule of keeping all but one variable constant and the experiment wouldn’t be fair. 1. this problem could be solved by using special sharp cutting tools like scalpels for cutting the beetroot roots and using a microgauge to accurately measure the sizes of the beetroot samples. ...read more.


almost equal to that of the test tube,so the discs would get stuck inside whilst trying to retrieve them with a mounted needle.this added to the time the disc should have stayed in the test tube, thus giving wrong results as the variable was not constant 5. discs with a smaller equal circumference could be used so that the disc is easily retrieved on time. 6. there was no control experiment against which i could compare my results to establish that temperature increase was responsible for increased diffusion of anthocyanin out of the cells. 6.a control could be provided. A beetroot disc in the distilled water without heating it for 30 minutes could be used as control. 7. Pooling the data caused high standard deviations as each student might have carried out the experiment in a slightly different way,thus making the mean unreliable 7.this could be solved if each student conducted a particular number of trials themselves so that the data obtained can be consistent thus giving a more reliable mean with low standard deviation. . ...read more.

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