• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in Hydrogen Peroxide Experiment.

Extracts from this document...

Introduction

The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in Hydrogen Peroxide Observations: Table 1: Physical Properties of Filter Paper Disks coated with Catalase Enzyme before and after Exposure to 3% hydrogen peroxide solution in Culture Tubes Before Exposure to 3% hydrogen peroxide After Exposure to 3% hydrogen peroxide Catalase-Coated Filter Paper Disks * White * Damp * Solid * Opaque * White * Damp * Solid * Fuzzy with bubbles 3% hydrogen peroxide solution (H2O2) * Liquid * Clear * Colourless * Liquid * Clear * Colourless Table 2: Time Taken for different temperature conditions of Enzyme Catalase coated onto Filter Paper Disks to travel to the top of 3% hydrogen peroxide solution in Culture Tubes Target Catalase Enzyme Temperature (�C) Trial Actual Temperature of Catalase (�0.5�C) Time (�0.1s) 0 1 0.3 4.0 2 2.9 3 3.5 4 3.6 5 4.4 20 1 22.0 3.4 2 3.6 3 3.1 4 4.1 5 3.1 40 1 39.8 3.8 2 3.9 3 3.6 4 4.6 5 6.1 60 1 60.1 16.4 2 12.2 3 18.8 4 16.6 5 7.9 80 1 81.3 NR for all trials 2 3 4 5 *NR = No Reaction Analysis: Sample Calculations: To calculate Relative Rate of Enzyme activity of Catalase: E.g. For Target 20oC Enzyme Catalase Temperature, Trial 1: To calculate Mean Relative Rate of Enzyme activity of catalase: E.g. For the target 20oC Enzyme Catalase Temperature Data Set: To calculate Standard Deviation: E.g. For Mean Relative Rate of Enzyme activity of catalase for the target 20oC Temperature condition of Catalase Data Set: Table 3: Relative ...read more.

Middle

All of the paper filter disks coated with enzyme catalase were placed in the same amount and temperature (in mL and filled to the same level in every culture tube with 2cm gap from top of culture tube) of 3% Hydrogen peroxide solution in culture tubes. All the filter paper disks coated with enzyme catalase were damp, opaque, white and solid (table 1) before exposure to 3% hydrogen peroxide solution and after exposure to 3% hydrogen peroxide they were fuzzy with bubbles on them, solid, damp and white (table 1). The fuzziness and bubbles indicate the reaction of the enzyme catalase coated onto the filter paper disk occurred with the 3% hydrogen peroxide in the culture tube and that oxygen was produced, which were the bubbles as it should according to the literature decomposition balanced equation of 3% hydrogen peroxide where enzyme catalase is the catalyst: . The 3% hydrogen peroxide was clear, colourless and liquid before and after the reaction with the filter paper disk coated with enzyme catalase (table 2) in the culture tubes. The observations and analysis (table 2 & 3 and figure 1) support the hypothesis of the relative reaction rate of catalase enzyme increasing up to a certain temperature (around 37�C for humans) then decrease past the optimal temperature (around 37�C in humans) because the enzyme begins to denature. Firstly, from temperatures 0-40�C (table 2) of the enzyme catalase coated onto filter paper disk there was a small increase in time taken (about 0.2-0.5 for each trial) ...read more.

Conclusion

into the 3% hydrogen peroxide solution before the culture tube was inverted. This caused the production of oxygen (bubbles) to happen sooner and since both sides of the filter paper disks are reacting in the beginning (as compared to only one side reacting at the beginning until it somewhat rises into the 3% hydrogen peroxide solution) it resulted in a decrease in amount of time taken to rise to them top of 3% hydrogen peroxide solution which increases relative reaction rate of the enzyme catalase. To fix this, dip only one paper filter paper disk into catalase enzyme solution at a time, and tap each side carefully using tweezers on paper towel and immediately place onto rubber stopper so they are still sticky. The enzyme catalase extract solution was obtained from a beaker. For the temperatures of 40�C, 60�C and 80�C the beakers were placed on hot plates to keep the temperature consistent. Since the beakers were left on the hot plates for a while, at high temperatures especially the 80�C condition, the water in the solution of the enzyme catalase can begin to evaporate and thus increasing the enzyme concentration in the solution. This causes the relative rate of enzyme activity of catalase to increase (meaning takes less time to flout to top of 3% hydrogen peroxide solution) as there are more enzymes to decompose hydrogen peroxide for the 40�C, 60�C and 80�C temperature conditions. To fix this problem, heat up the catalase enzyme extract solution to the desired temperature condition and use that catalase enzyme extract solution immediately to minimize evaporating of water. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Marked by a teacher

    Effect of substrate concentration on catalase activity (Biology IA)

    3 star(s)

    was added into the tube. 6. The bung was immediately placed back into the tube. 7. The stopwatch was started immediately and how long it took for the fluid in the U-shaped tube to rise by 5cm was timed. 8. The tube was occasionally agitated to help mix the contents.

  2. Browning Enzyme

    Borer - Ruler - Knife - Pipette - Tile Retrieving the Juice - Filter Paper - Another set of Twenty-Five test tubes - Crucible set ( with a pestol) - Twenty- Five cuvettes - Three Stands - Pipette - Measuring Cylinder - Colorimetre - Method 1) Take one apple 2)

  1. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    enzyme has reacted with the Hydrogen Peroxide, a gas syringe will be utilised to measure this. Apparatus: -Hydrogen Peroxide (10%) -Chicken Liver (Catalase) -Buffer solutions (pH 1, 4, 10, 14) -water bath -Test-tubes/beaker -measuring cylinders -pH probe -Test-tube stand -gas syringe -scalpel -cutting board -ruler -paper -pencil -electronic weighing machine

  2. How pH effects enzyme Catalase in potato cells

    There were several anomalies I found during experimentation, which was an advantage for I was able to correct and redo the tests, instead of lose and exclude them from my results. Therefore I have no anomalus data in my results table, now I will calculate the average time of each test and calculate the rate of catalase.

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    = = = 1.80 � 0.25mL For the rate of reaction (2% of hydrogen peroxide) = = = 0.06 Evaluation Weakness/Limitation Improvement 1. There was not enough time to repeat the experiments and to get the reliable results. Next time, the experiments should be repeated more than three times so that more reliable results can be collected.

  2. Influence of pH on the activity of potato catalase

    First 195.0 163.0 151.0 128.0 55.0 55.0 Second 198.0 172.0 162.0 158.0 59.0 64.0 Third 212.0 180.0 167.0 162.0 70.0 71.0 Fourth 221.0 191.0 172.0 169.0 80.0 82.0 STANDARD ERROR: 5.3 5.2 3.9 7.9 4.9 5.0 Rounded to 1 decimal place Table 5: Mean, standard deviation, range.

  1. Experimental Question: What effect does substrate concentration have on the rate of enzyme activity?

    Record the time in the observations table. 9. Repeat the procedure thrice for better results and record the time in the observations table. 10. Next, fill the test tube with 20mL of Hydrogen Peroxide (H2O2) and dilute it with 10mL of Distilled Water using the 100mL Graduated Cylinder.

  2. The Effect of pH on the activity of enzyme Catalase

    Place on the tile to cut into at least 30 discs prepared for replicas for each sets each 1 mm wide. 2. Place all the discs in small beaker of water. 3. Clamp a boiling tube to a stand and carefully insert the manometer into rubber bung.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work