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The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in Hydrogen Peroxide Experiment.

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Introduction

The Effect of Temperature on the Rate of Activity of the Enzyme Catalase in Hydrogen Peroxide Observations: Table 1: Physical Properties of Filter Paper Disks coated with Catalase Enzyme before and after Exposure to 3% hydrogen peroxide solution in Culture Tubes Before Exposure to 3% hydrogen peroxide After Exposure to 3% hydrogen peroxide Catalase-Coated Filter Paper Disks * White * Damp * Solid * Opaque * White * Damp * Solid * Fuzzy with bubbles 3% hydrogen peroxide solution (H2O2) * Liquid * Clear * Colourless * Liquid * Clear * Colourless Table 2: Time Taken for different temperature conditions of Enzyme Catalase coated onto Filter Paper Disks to travel to the top of 3% hydrogen peroxide solution in Culture Tubes Target Catalase Enzyme Temperature (�C) Trial Actual Temperature of Catalase (�0.5�C) Time (�0.1s) 0 1 0.3 4.0 2 2.9 3 3.5 4 3.6 5 4.4 20 1 22.0 3.4 2 3.6 3 3.1 4 4.1 5 3.1 40 1 39.8 3.8 2 3.9 3 3.6 4 4.6 5 6.1 60 1 60.1 16.4 2 12.2 3 18.8 4 16.6 5 7.9 80 1 81.3 NR for all trials 2 3 4 5 *NR = No Reaction Analysis: Sample Calculations: To calculate Relative Rate of Enzyme activity of Catalase: E.g. For Target 20oC Enzyme Catalase Temperature, Trial 1: To calculate Mean Relative Rate of Enzyme activity of catalase: E.g. For the target 20oC Enzyme Catalase Temperature Data Set: To calculate Standard Deviation: E.g. For Mean Relative Rate of Enzyme activity of catalase for the target 20oC Temperature condition of Catalase Data Set: Table 3: Relative ...read more.

Middle

All of the paper filter disks coated with enzyme catalase were placed in the same amount and temperature (in mL and filled to the same level in every culture tube with 2cm gap from top of culture tube) of 3% Hydrogen peroxide solution in culture tubes. All the filter paper disks coated with enzyme catalase were damp, opaque, white and solid (table 1) before exposure to 3% hydrogen peroxide solution and after exposure to 3% hydrogen peroxide they were fuzzy with bubbles on them, solid, damp and white (table 1). The fuzziness and bubbles indicate the reaction of the enzyme catalase coated onto the filter paper disk occurred with the 3% hydrogen peroxide in the culture tube and that oxygen was produced, which were the bubbles as it should according to the literature decomposition balanced equation of 3% hydrogen peroxide where enzyme catalase is the catalyst: . The 3% hydrogen peroxide was clear, colourless and liquid before and after the reaction with the filter paper disk coated with enzyme catalase (table 2) in the culture tubes. The observations and analysis (table 2 & 3 and figure 1) support the hypothesis of the relative reaction rate of catalase enzyme increasing up to a certain temperature (around 37�C for humans) then decrease past the optimal temperature (around 37�C in humans) because the enzyme begins to denature. Firstly, from temperatures 0-40�C (table 2) of the enzyme catalase coated onto filter paper disk there was a small increase in time taken (about 0.2-0.5 for each trial) ...read more.

Conclusion

into the 3% hydrogen peroxide solution before the culture tube was inverted. This caused the production of oxygen (bubbles) to happen sooner and since both sides of the filter paper disks are reacting in the beginning (as compared to only one side reacting at the beginning until it somewhat rises into the 3% hydrogen peroxide solution) it resulted in a decrease in amount of time taken to rise to them top of 3% hydrogen peroxide solution which increases relative reaction rate of the enzyme catalase. To fix this, dip only one paper filter paper disk into catalase enzyme solution at a time, and tap each side carefully using tweezers on paper towel and immediately place onto rubber stopper so they are still sticky. The enzyme catalase extract solution was obtained from a beaker. For the temperatures of 40�C, 60�C and 80�C the beakers were placed on hot plates to keep the temperature consistent. Since the beakers were left on the hot plates for a while, at high temperatures especially the 80�C condition, the water in the solution of the enzyme catalase can begin to evaporate and thus increasing the enzyme concentration in the solution. This causes the relative rate of enzyme activity of catalase to increase (meaning takes less time to flout to top of 3% hydrogen peroxide solution) as there are more enzymes to decompose hydrogen peroxide for the 40�C, 60�C and 80�C temperature conditions. To fix this problem, heat up the catalase enzyme extract solution to the desired temperature condition and use that catalase enzyme extract solution immediately to minimize evaporating of water. ...read more.

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