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The effect of varying temperature on the permeability of plasma membrane on beetroot.

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Introduction

BIOLOGY LAB 1 Name: Arrpita Sethi Candidate Number: Year: 2010-2012 Date: Title: The effect of varying temperature on the permeability of plasma membrane on beetroot. Research Question: How does varying temperature affect the permeability of the plasma membrane of beetroot cores? Hypothesis: Beetroot is a eukaryotic cell, and like other eukaryotic cells, beetroot cells have many types of cell organelles. Some of the organelles are bounded by a single membrane, e.g. lysosomes, Golgi apparatus, vacuoles; some are bounded by two membranes (an envelope), e.g. nucleus, mitochondrion.1 Beetroot has a betalain pigment, which makes it appear dark red or purple in colour. ...read more.

Middle

The tail is hydrophobic. "Hydro" means water "philic" means, "loving." "Phobic" means, "fearing." Thus hydrophobic means it doesn't attract water, while hydrophilic means it attracts water. The tails pack together, exposing only the polar heads to the water. 2 Apparatus: * Microscope * Beetroot cores * Water (varying temperatures) * Test tubes * Syringe Procedure: * Take a 2cm core of beetroot. * Fill 3/4th of the beaker with 10�C of water. * In the test tube, put core of beetroot and using a syringe pour 15ml of water into the test tube. ...read more.

Conclusion

57.60% 20 0.205 62.40% 30 0.203 62.60% 40 0.195 63.80% 50 0.210 61.70% 60 0.671 21.30% 70 0.846 14.30% Table 4: average absorbance TEMP �C Ab 10 0.218 20 0.204 30 0.179 40 0.2 50 0.262 60 0.514 70 0.851 Data Processing and evaluations: graph of absorbance Table 5: average transmittance TEMP �C Trans (%) 10.00 60.20 20.00 62.50 30.00 63.00 40.00 63.00 50.00 52.20 60.00 32.00 70.00 14.10 Data Processing and evaluations: graph of transmittance Conclusion and evaluation: As the temperature increases, the membrane gets denatured which means the membrane becomes more permeable. As the temperature increases absorbance increases while transmittance decreases. 1 http://answers.yahoo.com/question/index?qid=20061129041445AAaMGDT 2 http://vcebiology.edublogs.org/files/2011/02/450xCell_membrane_detailed_diagram_en_svg-os8fh4.jpg ...read more.

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