• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

The variation of rate of enzymatic activity of Bos primigenius liver with respect to surface area

Extracts from this document...

Introduction

The variation of rate of enzymatic activity of Bos primigenius liver with respect to surface area Introduction In this lab the rate of enzymatic activity will be tested by pouring hydrogen peroxide over cow liver. A reaction will take place its products are water and oxygen. The enzymatic activity rate will be compared between pieces of liver with different sizes, and the change in pressure in a bottle/container will be measured, which will indicate the enzymatic activity rate. Materials: * Device measuring pressure * Liver * Nalgene reaction bottle * Syringe without a needle * 3% solution of hydrogen peroxide * Scalpel * An electric scale * Tweezers * Computer with Logger Pro installed Independent Variable: * Mass of the liver piece Dependent Variable: * Change in pressure in the nalgene reaction bottle Controlled Variables: Variable controlled: How? Why? Shape of the liver piece Rectangular The increase in mass will result from an increase in size, so that the surface ...read more.

Middle

Put a piece of liver into the pyramid-like tube 4. Load the syringe with 15 mL of hydrogen peroxide 5. Press "start experiment" in LogoPro and pour the hydrogen peroxide into the nalgene reaction bottle and cover it with the device measuring pressure 6. Wait until the computer program finishes recording data 7. Save the data on the computer 8. Clean the tube 9. Repeat steps 3-8 with all of the liver pieces Safety gear: * Goggles Data Collection and Processing Graph #1. 1g cow liver pieces X-axis - time (s). Y-axis - pressure (kPa) In every trial, there was a drop in the beginning, followed by a steady raise in pressure. Trial #2 and #3 were very similar, even though #3 had a bigger drop in pressure in the beginning. Average ending pressure was 92.61 kPa. Graph #2. 1.5g liver pieces X-axis - time (s). ...read more.

Conclusion

* Liver pieces were not similar enough in shape. Some were thinner and longer, while other were thicker and shorter, resulting in different surface areas and therefore uncertainty in data. * The measuring device was not of 100% precision and accuracy. It sometimes recorded the same amount of pressure for 2-4 seconds, and the starting pressure was not always the same. * The speed of pouring in the H2O2 was not the same, resulting into some data having large spikes in the beginning of the trial * Timing between trials of starting recording and pouring in the H2O2 was not same. This could have affected the data received, because the trials had different reaction times Conclusion A raise in pressure and difference in data between treatments proves that this method is viable and can be used for such types of experiments. The data suggests that an increase in surface area results in greater enzymatic activity. However, the amount of errors prevented a solid confirmation. These errors introduced uncertainties, which greatly affected the data. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

    Up to this point the line of best fit is a straight line through the origin. This shows that without the enzyme, catalase, present no reaction takes place. It also indicates that the enzyme concentration is directly proportional to the rate of reaction for the decomposition of hydrogen peroxide in

  2. Modelling Surface Area to Volume Ratio in Cells with Agar Cubes

    Repeat these steps for the agar cubes sizes 8cm3 and 27cm3 Results Table 1 The penetration percentage (%) of hydrochloric acid (HCL-0.1M) when cubes of bromothymol blue agar (side lengths 3cm, 2cm, 1cm) with increasing surface area to volume ratio's (2:1, 3:1, 6:1)

  1. Human variation

    Length of hand span (cm) (�1) Height (cm) (�2) No. Length of hand span (cm) (�1) Height (cm) (�2) 1 16.5 145.0 14 19.2 153.5 2 17.4 165.5 15 19.5 167.3 3 18.0 171.4 16 19.6 165.0 4 18.3 166.0 17 20.0 169.0 5 18.5 169.9 18 20.2 173.0 6

  2. Enzyme activity

    Dependent The time taken for the reaction to occur will be timed until the solution has changed colour (brown indication of lack of starch) in the iodine for each dimple tray after every 15 seconds on the reaction. Controlled The ratio of amylase and starch solution used will be 1:3.

  1. Investigating the effect of pH of Hydrogen Peroxide upon the activity of Catalase

    The volume of the buffer solutions used shall also be regulated so that it is constant. Again, a measuring cylinder will be used to measure the amount used to 5ml before it is added to the Hydrogen Peroxide. To measure the amount of oxygen that is diffused after the Catalase

  2. LAB-What infuences blood pressure

    - sphygmomanometer, - pen, - piece of paper, - weight, - measure line, - tape measure, Procedure: 1. Select large group of people on the same age (100 or more), for example 18 years old, 2. Measure their blood pressure (systolic and diastolic) in rest twice by using sphygmomanometer, 3.

  1. Genetic Variation

    Variation is important because without it, a species will be same all around with the same flaws and it will have less of a chance of survival. In order for there to be variation, there must be a population and not just a few individuals.

  2. How does the concentration of hydrogen peroxide (substrate) affect the enzymatic activity of catalase ...

    Measuring cylinders will be used for accurate measurement. Type of substrate & enzyme The type of substrate and enzyme has to be the same for the whole experiment to work. The substrate is the hydrogen peroxide and the enzyme is catalase, which is obtained from carrots. Equipment Equipment such as measuring cylinders, conical flasks and gas syringes will need to be the same.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work