• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month
Page
1. 1
1
2. 2
2
3. 3
3
4. 4
4
5. 5
5
6. 6
6

# Title: separation of pigments of photosynthesis using paper chromatography. Goal (main aim): Calculating the Rf of every single pigment, in order to distinguish it and identify its solubility.

Extracts from this document...

Introduction

Chromatography Experiment Title: separation of pigments of photosynthesis using paper chromatography. Goal (main aim): Calculating the Rf of every single pigment, in order to distinguish it and identify its solubility. Hypothesis: In photosynthesis; two types of pigments are involved; chlorophyll (A or B) and carotenoids, by using paper chromatography we can distinguish each pigment, by measuring the color's Rf; referring to the next formula: Variables: Independent variable: - Pigment solubility. Dependent variable: - The distance that the pigments move, on the chromatography paper. Controlled variables: VARIABLE WAY OF CONTROL Temperature The experiment will be carried out in room temperature. Solvent volume In order to minimize uncertainty; the solvent will be added using a pipette. Volume & concentration of extraction The extraction sample will be taken using the same tweezers. ...read more.

Middle

- Insert the chromatography paper (containing the pigments of the extraction), in the chromatography jar until the triangular end is totally emerged by the solvent. (Keep in mind not to let the extraction pigments touch the solvent). - Close the jar firmly, to prevent solvent evaporation. - Wait, until the pigments start dissolving, as a result of the solvent elevating on the paper. - When the solvent is 1 cm away from your paper's top, take the papers out and mark the farthest point of the solvent's elevation (solvent front). (Make sure you mark quickly before the line evaporates). - Repeat the previous steps for more results. - Calculate Rf using the following formula: - Put your results in the form of a table. ...read more.

Conclusion

and (X2)(0.70�0.05), can be considered close to the standard (Rf) (0.45) and (0.65) respectively, which shows that the method used to calculate (Rf ) in the hypothesis is valid. Evaluation: * In table (1), a clear difference can be seen between the reults of the 1st and 2nd trials, and also between the average (Rf) of (X3) and the standard (Rf), which shows the occurrence of errors, those errors could be: - To cut a triangular end, a pair of scissors was used; using a pair of scissors doesn't always guarantee the formation of an isosceles triangle, which affected the elevation (diffusion), of the solvent up the paper. - Since tweezers were used to add the extraction samples, it had a huge possibility of uncertainty when it comes to the equality of the volume taken for the two trials. - A ruler was used to measure the distance moved by each pigment, and this contains a pretty much big amount of uncertainty involved. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

## Found what you're looking for?

• Start learning 29% faster today
• 150,000+ documents available
• Just £6.99 a month

Not the one? Search for your essay title...
• Join over 1.2 million students every month
• Accelerate your learning by 29%
• Unlimited access from just £6.99 per month

# Related International Baccalaureate Biology essays

1. ## DNA Strawberry Extraction Lap

it with a microscope; we can see that the clumped mass is composed of interlocking strands that are "woven" together. 5) Would you expect scientist to use the exact same method to extract DNA from human tissue? Why or why not?

2. ## Chromatography lab. Title: separation of pigments of photosynthesis using paper chromatography.

- By tweezers, take an extraction sample, put in on the center of the start line. - Wait until the pigments of the sample are absorbed. - By a pipette, pour 1 mm (approx.) of the solvent, into the chromatography jar.

1. ## Chromatography of photosynthetic pigments. Aim: To determine the different photosynthetic pigments found in ...

This is because of the presence of an abundant amount of chlorophylls in the chloroplasts of plant cells. These chlorophyll pigments reflect green wavelengths of light and absorb the other visible wavelengths of light.

2. ## design photosynthesis

time taken for each ananas comosus leaf disc to float on surface of sodium hydrogen carbonate solution is measured using stopwatch The rate of the process is calculated using formulae: Rate =1/Time c) Controlled variables : a. Type and species of leaves i.

1. ## Identification of Organic Compounds

Place on a hot plate and heat to boiling. 2. Using the following steps, test each of the substances in a table for the presence of reducing sugars. a. Number clean test tube 1 - 10 b. To each test tube, add 1 mL of substance to be tested c.

2. ## Photosyntetic Pigments

With it we try to show that which solar radiation have a different reaction at our plant. To do it we needed: * 4 Colors of Cardboard (Red, Blue, Yellow, Black) * 4 Colors of Cellophane (Red, Blue, Yellow, Transparent with double layers)

• Over 160,000 pieces
of student written work
• Annotated by
experienced teachers
• Ideas and feedback to