• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

What is the color of pigment that can be found in green plants at different wavelength using a colorimeter?

Extracts from this document...

Introduction

Name: Muhammad Izzuddin bin Ab Aziz Class: M11P Title: Colors of Light (Wavelengths) Absorbed By Green Plants Introduction: Refer attachment. Objective: Refer attachment. Research question: What is the color of pigment that can be found in green plants at different wavelength using a colorimeter? Materials: Refer attachment. Method: Refer attachment. Data collection: Data collection: Qualitative data: Wavelength (nm) Absorbance of light (abs) (±0.01) Mustard Spinach Trial 1* Trial 2 Trial 3 Trial 4 Trial 5 Trial 1* Trial 2 Trial 3 Trial 4 Trial 5 440 2.00 1.98 2.00 2.00 1.74 2.00 0.39 0.82 0.48 0.35 470 1.85 1.74 1.95 1.69 1.22 1.44 0.46 0.65 0.25 0.26 490 0.57 1.67 0.85 0.49 0.49 0.54 0.38 0.40 0.20 0.19 520 0.35 0.63 0.62 0.25 0.38 0.34 0.31 0.38 0.13 0.21 550 0.17 0.32 0.55 0.41 0.38 0.21 0.29 0.40 0.18 0.26 580 0.20 0.35 0.76 0.39 0.41 0.08 0.22 0.36 0.10 0.16 590 0.57 0.34 1.05 0.40 0.36 0.37 0.13 0.33 0.07 0.10 680 0.78 0.47 1.23 0.80 0.44 0.54 0.16 0.40 0.14 0.11 Table 1: The absorbance of light for mustard and spinach in different wavelength. ...read more.

Middle

by mustard leaves extraction. Wavelength of light (nm) Average absorbance of wavelength of light (Abs) Standard deviation of average absorbance of light (Abs) Percentage average absorbance of wavelength of light (%) ± standard deviation (%) 440 1.944 0.11 32.09 ± 0 470 1.690 0.28 28.6± 2.18 490 0.814 0.50 13.7 ± 1.15 520 0.446 0.17 7.60± 0.26 550 0.366 0.19 5.32 ± 2.53 580 0.422 0.29 7.05 ± 1.16 590 0.544 0.31 7.82 ± 1.94 680 0.744 0.61 8.83 ± 2.92 1. To find mean of absorbance of wavelength of light (%) = xn =sum of absorbance of wavelength of light for a certain wavelength no of trials For example: Mean of the absorbance of light for 440nm of spinach leaves =0.65+0.57+0.483 = 0.92 Abs 1. To find standard deviation of the mean absorbance of light = (x-xËË)2n-1 x=class value xË=mean of absorbance of wavelength of light n=no oftrials For example : Standard deviation of mean absorbance of light of 440nm wavelength of spinach leaves = (0.65-0.57)2+(0.65-0.57)2+(0.65-0.57)23-1 = 0.83 1. Percentage average absorbance of wavelength of light (%): = average absorbance of wavelength x 100% Total average relative absorbance of wavelength Example: To find percentage average of absorbance of wavelength 440nm ...read more.

Conclusion

It also shows that the pigment needs the colour more than the colour that the absorption is low. 1. The low absorption of certain colour is happened because the pigment already has the colour in it. Evaluation: Limitation Suggestion 1. When extracting the supernatant after centrifugation process is done, some of the sediment in the bottom layer is accidently taken too. Avoided by using syringe to make sure no sediment will be taken in the supernatant. 1. Parallax error happens when taking the acetone using a measuring cylinder. The position of eyes must be perpendicular to the reading. The uses of white paper to be placed behind the measuring cylinder also will increase the accuracy of the reading. 1. The extract is exposed to the light for a short period of time before it was wrapped by aluminum foil. The experiment should be conducted in the dark room where there is no sunlight at all. Besides, after the extract is obtained, the centrifuged fuse should be directly wrapped by aluminum foil. 1. The freshness of the leaves is not the same. The leaves are picked up from the tree exactly before the experiment want to be conducted by the students. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. DNA Strawberry Extraction Lap

    Bananas - Some are modified to produce human vaccines against infectious diseases such as hepatitis B. Fruit and nut trees yield years earlier and plants that produce new plastics with unique properties. Conclusion DNA can be found in all living organisms such as humans, animals, plants and fruits.

  2. Penicillin - its discovery, properties and uses.

    The first relatively useful antibiotic was probably Salvarsan, a drug discovered in 1909 by Paul Ehrlich. Salvarsan was a compound made from arsenic that was effective in the treatment of syphilis and other diseases caused by spirochete bacteria.

  1. Experiment Colours of Light (Wavelength) absorbed by green plant

    Different organisms may not have the materials needed to absorb the wavelength. Mass of plant The experiment is conducted with both plants; mustard and spinach, have an approximate value of mass at 5.0 g. Different mass of plants may lead to a highly different absorbance.

  2. Allelopathy. Open Investigation Will increasing the number of allelopathic sunflower plants effect the ...

    The size of seeds planted Seeds will the same of very similar size will be used in this experiment. Broken seeds will be neglected. The materials used to collect data The same 30cm plastic ruler will be used throughout the experiment to measure the size of the leaves and the

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work