• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Yeast Lab

Extracts from this document...

Introduction

Investigation and analyzing the effects of temperature change on anaerobic cellular respiration of the yeast cells Rashiv Lubana Biology 20-IB December 12th 2008 Mr. Myskiw Investigation and analyzing the effects of temperature change on anaerobic cellular respiration of the yeast cells. Introduction: Yeast (Saccharomyces cerevisiae) is a Eukaryotic microorganism and is classified into Kingdom Fungi. Yeasts are chemoorganotrophs which means that they use organic compounds as a source of energy and do not necessarily require sunlight and oxygen to grow. Yeast instead takes sugars such as sucrose and fructose for energy. Yeast needs energy to carry out different functions during their lifetime. They obtain this energy through cellular respiration, which involves the oxidation of organic molecules. Some of the energy produced by oxidation is stored in the chemical bonds of adenosine triphosphate (ATP) - the energy currency of the cell. There are two types of cellular respirations which are being carried out in the environment. First Aerobic respiration, it is the respiration which can be performed by all the living things in the presence of air. On the other hand anaerobic respiration is performed in the absence of oxygen and only some microorganisms are capable of performing it. ...read more.

Middle

Health of the yeast cells should be fairly similar. They both should be healthy in terms of producing ATP. Same and good quality of test tubes and water tubes should be taken in order to prevent the breaking and tearing respectively. Time is the biggest controlled variable, which has to control to get the amount of carbon dioxide. Pressure is maintained throughout the lab. Same kinds of rubber knobs are used. Design: ATP and CO2 are both the products of cellular respiration. It is not easy to find the amount of ATP produced during cellular respiration. So measuring the amount of carbon dioxide produced during anaerobic respiration will indirectly allow the experimenter to find the amount of ATP produced by the yeast cell. Samples of the yeast will be taken from the same location. Sucrose will be added to them and will be kept in different temperature for a particular time. Carbon dioxide will be collected in the water tube and the volume of carbon dioxide will be found with the Ideal gas law. Materials required: In order to complete the experiment some materials are required. Safety goggles, apron and oxygen mask for safety. To take the yeast samples tongs are required, along with the scoopula. ...read more.

Conclusion

The formula for Ideal gas law is PV=NRT. For our values P is the pressure at STP and it is 101.325. R is the universal gas law constant which is 8.3145 m3�Pa/(mol�K). T is the temperature of the oven or the temperature at which respiration was happening. N is amount of substance, in mol and which is calculated by dividing the mass of carbon dioxide by the molar mass of carbon dioxide. After calculating the number of moles substitute the values of P, N, R and T and solve for V. By this experimenter would be able to find the volumes at different temperatures. The amount of carbon dioxide will indirectly tell experimenter the amount of ATP. Sample number Weight of the water tube Weight of CO2 (weight of water tube with CO2 - weight of water tube) Number of moles Mass of CO2 / molar mass of CO2 Amount of CO2 produced V=NRT/P 1 2 3 4 5 Sources "BBC - Education Scotland - Standard Grade Bitesize Revision - Ask a Teacher - Biology - Biotechnology - Question From ." BBC - Homepage. 10 Nov. 2008 <http://www.bbc.co.uk/scotland/education/bitesize/standard/other/sos/biology/biotechnology/biotechnology_16.shtml>. Papers - The Effect of Temperature on the Respiration of Yeast." Search Essays -- Free Essays and Term Papers. 9 Nov. 2008 <http://www.123helpme.com/view.asp?id=122462>. Inquiry into BIOLOGY. Ontario: Keith Owen Richards, 2007. ?? ?? ?? ?? By Rashiv Lubana ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Vitamin C Lab

    However, there is an exception with the titrations of room temperature lemon juice. The reason for this could be environmental - the classroom's temperature could have fluctuated, hence altering the lemon juice and its Vitamin C concentration. Another reason why the mean titre of lemon juice at 25�C (room temperature)

  2. The effect of the tempereature on yeast metabolism.

    the yeast increased to a certain point where, as the temperature rose to a certain level which in my case was 60oC, the rate of respiration drops down as shown on my graph (figure 1). My hypothesis is supported by these findings and on the graph and tables (table 1, table 2)

  1. Plant Tropism Lab

    This proves that bean plants grow against gravity, even when external forces suddenly change the orientation of the plants, they still grow upright. However, the data's standard deviation is too high to make a valid conclusion. On Charts 3.1 - 3.5, one can see that the standard deviation of each

  2. biology extended essay - How different diets: vegetarian, vegan and a meat centered diet ...

    162.5cm 160cm 175cm 168cm 155cm Are you sick now? no no yes(cold) no yes(cold) When last did you have a cold? In june this year In january this year Have it right now In april this year Have it right now How often do you have a cold? Once to Twice a year Two to three times a year

  1. Biology Industrial Melanism of Peppered Moth Lab

    As the Industrial Revolution progressed, soot and smoke started to build up on trees and lichens (moth habitat). The melanic species of moths are better adapted than the light-coloured moths because of their ability to camouflage with the darken trees and lichen.

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    at the reading of the 1.0 ml pipettes of the respirometres for 30 minutes in room temperature. The pipettes each contain, germinating seeds, non-germinating seeds and bead. Qualitative Description: The germinating seeds were yellowish green, round and slightly wrinkled. They were also wet because they were being germinated for 24 hours.

  1. Beet Lab. Aim: To determine the effects of temperature on the permeability of ...

    x 100 Apparatus Uncertainty % Error Stopwatch 10+0.01 s (0.01/10) x 100 % = 0.1% Ruler 3+0.1 cm (0.1/3) x 100 % = 3.33% Syringe 25+0.01 ml (0.01/25) x 100 % = 0.04% Thermometer 20 + 0.5 oC 30 + 0.5 oC 40 + 0.5 oC 50 + 0.5 oC

  2. How does changing the percentage of sucrose added to yeast affect the rate of ...

    Comment on the graph: The calculated standard deviation and error bars are for three of my results (0%, 10% and 15%) were small, indicating that these the values were close to the mean and therefore valid. However at 5% sucrose, the error bar is very large (1SD is 0.028 mm)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work