• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Yeast Lab

Extracts from this document...


Investigation and analyzing the effects of temperature change on anaerobic cellular respiration of the yeast cells Rashiv Lubana Biology 20-IB December 12th 2008 Mr. Myskiw Investigation and analyzing the effects of temperature change on anaerobic cellular respiration of the yeast cells. Introduction: Yeast (Saccharomyces cerevisiae) is a Eukaryotic microorganism and is classified into Kingdom Fungi. Yeasts are chemoorganotrophs which means that they use organic compounds as a source of energy and do not necessarily require sunlight and oxygen to grow. Yeast instead takes sugars such as sucrose and fructose for energy. Yeast needs energy to carry out different functions during their lifetime. They obtain this energy through cellular respiration, which involves the oxidation of organic molecules. Some of the energy produced by oxidation is stored in the chemical bonds of adenosine triphosphate (ATP) - the energy currency of the cell. There are two types of cellular respirations which are being carried out in the environment. First Aerobic respiration, it is the respiration which can be performed by all the living things in the presence of air. On the other hand anaerobic respiration is performed in the absence of oxygen and only some microorganisms are capable of performing it. ...read more.


Health of the yeast cells should be fairly similar. They both should be healthy in terms of producing ATP. Same and good quality of test tubes and water tubes should be taken in order to prevent the breaking and tearing respectively. Time is the biggest controlled variable, which has to control to get the amount of carbon dioxide. Pressure is maintained throughout the lab. Same kinds of rubber knobs are used. Design: ATP and CO2 are both the products of cellular respiration. It is not easy to find the amount of ATP produced during cellular respiration. So measuring the amount of carbon dioxide produced during anaerobic respiration will indirectly allow the experimenter to find the amount of ATP produced by the yeast cell. Samples of the yeast will be taken from the same location. Sucrose will be added to them and will be kept in different temperature for a particular time. Carbon dioxide will be collected in the water tube and the volume of carbon dioxide will be found with the Ideal gas law. Materials required: In order to complete the experiment some materials are required. Safety goggles, apron and oxygen mask for safety. To take the yeast samples tongs are required, along with the scoopula. ...read more.


The formula for Ideal gas law is PV=NRT. For our values P is the pressure at STP and it is 101.325. R is the universal gas law constant which is 8.3145 m3�Pa/(mol�K). T is the temperature of the oven or the temperature at which respiration was happening. N is amount of substance, in mol and which is calculated by dividing the mass of carbon dioxide by the molar mass of carbon dioxide. After calculating the number of moles substitute the values of P, N, R and T and solve for V. By this experimenter would be able to find the volumes at different temperatures. The amount of carbon dioxide will indirectly tell experimenter the amount of ATP. Sample number Weight of the water tube Weight of CO2 (weight of water tube with CO2 - weight of water tube) Number of moles Mass of CO2 / molar mass of CO2 Amount of CO2 produced V=NRT/P 1 2 3 4 5 Sources "BBC - Education Scotland - Standard Grade Bitesize Revision - Ask a Teacher - Biology - Biotechnology - Question From ." BBC - Homepage. 10 Nov. 2008 <http://www.bbc.co.uk/scotland/education/bitesize/standard/other/sos/biology/biotechnology/biotechnology_16.shtml>. Papers - The Effect of Temperature on the Respiration of Yeast." Search Essays -- Free Essays and Term Papers. 9 Nov. 2008 <http://www.123helpme.com/view.asp?id=122462>. Inquiry into BIOLOGY. Ontario: Keith Owen Richards, 2007. ?? ?? ?? ?? By Rashiv Lubana ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Biology essays

  1. Vitamin C Lab

    However, there is an exception with the titrations of room temperature lemon juice. The reason for this could be environmental - the classroom's temperature could have fluctuated, hence altering the lemon juice and its Vitamin C concentration. Another reason why the mean titre of lemon juice at 25�C (room temperature)

  2. Yeast fermentation

    The CO2 will displace the remaining air. This flask will not contain oxygen after the first hour or two of the experiment. Shake up the culture in the flask to mix the yeast cells evenly. This is important in order to get consistent results.

  1. Biology lab- osmotic pressure

    0.19 ? (34 + 273) = 4.78 atmospheres 273 Evaluation The procedure of the investigation provides a reliable method for deducing the osmotic pressure at which plant cells are 50% plasmolysed in sucrose solution. The hypothesis that we had formulated has been verified through this experiment.

  2. Yeast lab. Question: how does the yeast concentration affect the rate of anaerobic ...

    (�50 ppm) Average difference (ppm) Trial (1) Trial (2) Trial (3) 2% 7830 11107 12352 7096.33 4% 16763 14974 21083 17606.66 6% 29296 29966 24896 28052.66 8% 30188 34553 27729 30823.33 10% 50919 36693 49421 45677.66 The previous data can be represented by the following graph: Graph (1): the CO2 yield vs.

  1. biology extended essay - How different diets: vegetarian, vegan and a meat centered diet ...

    Long distance walks daily Once a week Once a week(crippled) Walk to school everyday Not at all What is your blood pressure? 112/64 130/84 87/60 113/64 120/60 What is your body mass index? 20 16.7 18.2 19.6 20.6 How long have you had this diet?

  2. Bio lab - Oxygen Consumption in germinating and non-germinating seeds

    0.92 0.73 0.88 15 0.91 0.66 0.87 20 0.90 0.60 0.86 25 0.89 0.54 0.86 30 0.89 0.48 0.86 Raw Data Table 9: The volume of the water level at the reading of the 1.0 ml pipettes of the respirometres for 30 minutes in room temperature.

  1. Bread lab. What effect will the mass of glucose added to yeast have on ...

    Arrange the dough so that it is compressed enough so that the top of the dough is at 50 mL. 14) Place a damp cloth at the opening of the beaker. 15) Place the beaker in a 380C incubator and allow for it to rise for 30 minutes measured by a stop watch.

  2. Beet Lab. Aim: To determine the effects of temperature on the permeability of ...

    Average Standard Deviation 20oC 0.342 0.060411092 30oC 0.3898 0.05738641 40oC 0.4012 0.135673137 50oC 0.5486 0.234230229 60oC 1.0898 0.244183742 Transmission % Table Temperature Average Standard Deviation 20oC 45.78 % 6.512833485 30oC 41.56 % 5.325692443 40oC 41.28 % 13.28803974 50oC 31.3 % 12.20102455 60oC 16.62 % 14.45880355 Uncertainty calculations of the apparatus % error is calculated by (uncertainty/value)

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work