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Yeast Lab

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Investigation and analyzing the effects of temperature change on anaerobic cellular respiration of the yeast cells Rashiv Lubana Biology 20-IB December 12th 2008 Mr. Myskiw Investigation and analyzing the effects of temperature change on anaerobic cellular respiration of the yeast cells. Introduction: Yeast (Saccharomyces cerevisiae) is a Eukaryotic microorganism and is classified into Kingdom Fungi. Yeasts are chemoorganotrophs which means that they use organic compounds as a source of energy and do not necessarily require sunlight and oxygen to grow. Yeast instead takes sugars such as sucrose and fructose for energy. Yeast needs energy to carry out different functions during their lifetime. They obtain this energy through cellular respiration, which involves the oxidation of organic molecules. Some of the energy produced by oxidation is stored in the chemical bonds of adenosine triphosphate (ATP) - the energy currency of the cell. There are two types of cellular respirations which are being carried out in the environment. First Aerobic respiration, it is the respiration which can be performed by all the living things in the presence of air. On the other hand anaerobic respiration is performed in the absence of oxygen and only some microorganisms are capable of performing it. ...read more.


Health of the yeast cells should be fairly similar. They both should be healthy in terms of producing ATP. Same and good quality of test tubes and water tubes should be taken in order to prevent the breaking and tearing respectively. Time is the biggest controlled variable, which has to control to get the amount of carbon dioxide. Pressure is maintained throughout the lab. Same kinds of rubber knobs are used. Design: ATP and CO2 are both the products of cellular respiration. It is not easy to find the amount of ATP produced during cellular respiration. So measuring the amount of carbon dioxide produced during anaerobic respiration will indirectly allow the experimenter to find the amount of ATP produced by the yeast cell. Samples of the yeast will be taken from the same location. Sucrose will be added to them and will be kept in different temperature for a particular time. Carbon dioxide will be collected in the water tube and the volume of carbon dioxide will be found with the Ideal gas law. Materials required: In order to complete the experiment some materials are required. Safety goggles, apron and oxygen mask for safety. To take the yeast samples tongs are required, along with the scoopula. ...read more.


The formula for Ideal gas law is PV=NRT. For our values P is the pressure at STP and it is 101.325. R is the universal gas law constant which is 8.3145 m3�Pa/(mol�K). T is the temperature of the oven or the temperature at which respiration was happening. N is amount of substance, in mol and which is calculated by dividing the mass of carbon dioxide by the molar mass of carbon dioxide. After calculating the number of moles substitute the values of P, N, R and T and solve for V. By this experimenter would be able to find the volumes at different temperatures. The amount of carbon dioxide will indirectly tell experimenter the amount of ATP. Sample number Weight of the water tube Weight of CO2 (weight of water tube with CO2 - weight of water tube) Number of moles Mass of CO2 / molar mass of CO2 Amount of CO2 produced V=NRT/P 1 2 3 4 5 Sources "BBC - Education Scotland - Standard Grade Bitesize Revision - Ask a Teacher - Biology - Biotechnology - Question From ." BBC - Homepage. 10 Nov. 2008 <http://www.bbc.co.uk/scotland/education/bitesize/standard/other/sos/biology/biotechnology/biotechnology_16.shtml>. Papers - The Effect of Temperature on the Respiration of Yeast." Search Essays -- Free Essays and Term Papers. 9 Nov. 2008 <http://www.123helpme.com/view.asp?id=122462>. Inquiry into BIOLOGY. Ontario: Keith Owen Richards, 2007. ?? ?? ?? ?? By Rashiv Lubana ...read more.

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