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Investigate one factor affecting the rate of enzymatic decomposition of hydrogen peroxide by catalase.

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Introduction

The enzymatic Decomposition of Hydrogen peroxide Naoto OKAWA Aim: To investigate one factor affecting the rate of enzymatic decomposition of hydrogen peroxide by catalase. Hypothesis: Bigger the volume of the catalase, faster the rate of reaction Safety: Due to the hydrogen peroxide is corrosive we need the protection for example glasses and gloves Safety Precautions. Avoid any contact with hydrogen peroxide it will burn our skin. If any contact with hydrogen peroxide, wash the part where get contact painstakingly with water and report to teacher. Variables: Independent Variable - Volume of the potato Cut the potato into different sizes, dimensions of the potato pieces were equal Dependent Variable - Time taken Fill the test tube with a little bit of hydrogen peroxide. ...read more.

Middle

Carefully make the potato to pieces by using mold make 5 of it 3. Measure the dimensions of the potato pieces with the ruler 4. Measure 5ml of hydrogen peroxide with the measuring cylinder 5. Pour 5ml of hydrogen peroxide into the test tube 6. Release one piece of potato into the test tube 7. Start the stopwatch as soon as the potatoes get inside test tube 8. Stop the stopwatch when the oxygen bubbles reach the brim of the test tube 9. Record the time taken 10. Repeat the steps 4~9 for the remaining four pieces. Data Table - Volume (cm3) Time (sec) Rate (1/sec) 0.250 11.37 0.09 0.624 9.48 0.11 1.575 7.68 0.13 2.312 5.98 0.17 3.220 4.85 0.21 Conclusion: My hypothesis was right. ...read more.

Conclusion

I recorded the time taken with the stopwatch to two decimal places. I measured 5ml of hydrogen peroxide with 10ml measuring cylinder as soon as possible because they might react with something in the air and change. Also all the controlled variables were controlled. No anomalous results are shown in my graph and table. There are several stuffs that I can improve next time. One is, to increase the reliability of results, I could have put thermometer to measure the room temperature. This must be controlled to make it a fair experiment because reaction will easily get changes by dim temperature difference. Evaluation of results: The results of my experiment were precise. As the graph shows, no anomalous results were found. This indicates that the experiment was enough fair and went successful. Due to the topic of this experiment is biology the experiment, 'The Enzymatic Decomposition of Hydrogen Peroxide' falls on Health and Social Education in AOI. ...read more.

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