Controlled Variable -
Materials
Reagent-
Equipment-
- Test tube
- Stop-watch
- Mold
- Measuring cylinder
- Goggles
- Gloves
Method:
Procedures-
1. Wear the goggles and gloves
2. Carefully make the potato to pieces by using mold make 5 of it
3. Measure the dimensions of the potato pieces with the ruler
4. Measure 5ml of hydrogen peroxide with the measuring cylinder
5. Pour 5ml of hydrogen peroxide into the test tube
6. Release one piece of potato into the test tube
7. Start the stopwatch as soon as the potatoes get inside test tube
8. Stop the stopwatch when the oxygen bubbles reach the brim of the test tube
9. Record the time taken
10. Repeat the steps 4~9 for the remaining four pieces.
Data Table -
Conclusion:
My hypothesis was right. There was a direct relationship between the volume of the potato and rate of reaction and the rate of reaction increases with the size of the liver piece. In graph 2, the equation found out, y=0.0391x+0.077, with a R2 value of 0.9891 shows that the line of a increasing function. However, this equation would not apply when the size of potato get massive. The line in the graph will eventually reach to a plateau. This is when the volume of potato reaches at some amount or more than hydrogen peroxide, the rate of reaction would not increase any further due to the saturation of the enzyme-substrate complex. Two equations here were made based on my results. However, as the graph will reach to a curve and to then a plateau, the equations cannot be used.
Evaluation of method:
Based on the result, my hypothesis is reasonable and reliable. I recorded the time taken with the stopwatch to two decimal places. I measured 5ml of hydrogen peroxide with 10ml measuring cylinder as soon as possible because they might react with something in the air and change. Also all the controlled variables were controlled. No anomalous results are shown in my graph and table. There are several stuffs that I can improve next time. One is, to increase the reliability of results, I could have put thermometer to measure the room temperature. This must be controlled to make it a fair experiment because reaction will easily get changes by dim temperature difference.
Evaluation of results:
The results of my experiment were precise. As the graph shows, no anomalous results were found. This indicates that the experiment was enough fair and went successful. Due to the topic of this experiment is biology the experiment, 'The Enzymatic Decomposition of Hydrogen Peroxide' falls on Health and Social Education in AOI.