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Lactase Enzyme Lab Design

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Lactase Enzyme Lab Introduction: Lactose, the sugar found in milk, is a disaccharide composed of glucose and galactose (both six-sided sugars). Glucose is a six-sided sugar. Lactase is an enzyme that breaks lactose down into galactose and glucose. Lactase can be purchased in pill form by people who are lactose intolerant. These people lack the enzyme, lactase, and cannot break down the sugar lactose into its component parts. Question: How does the temperature of the lactose solution affect the process that Lactose goes through to break down Lactose? ...read more.


will take the least time to get broken down into galactose and glucose. This is because rise in temperature increases activity of the enzyme, as the higher the temperature the more kinetic energy the enzyme and the substrate molecules have. This means that more enzyme substrate complexes are likely to form. The increase in movement of the molecules enables a higher chance of the substrate molecules colliding with the enzymes molecules, with sufficient energy, to overcome activation energy, and therefore combining at the active site. Materials: Lactase drops 4 glucose reagent strips 4 test tubes Stirring rod Thermometer Graduated cylinder Milk samples at different temperatures (20F, 40F, 60F, 80F, 100F) ...read more.


3. Get one sample of milk from the prep table; using only 2 ml. Record the temperature 4. Use a reagent strip to measure the glucose concentration of the milk and record. (throw away the used strip) 5. Add one drop of lactase to the test tube. Shake or stir to be sure that it is evenly distributed. Wait 3 minutes. Measure the glucose concentration again. Record the measurement and throw away the used strip. 6. Repeat with the three other milk samples. 7. Don't forget to take multiple trials (3-5). 8. When you finish collecting data, don't forget to clean the work station ?? ?? ?? ?? Mayur Bedadala Pd. 2 AP/IB Biology ...read more.

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