• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Research Question Will increasing the temperature (C) of the rennin enzyme affect the rate of reaction (seconds) at which Pauls full cream milk solidifies?

Extracts from this document...

Introduction

High Level Chemistry Design Practical Investigate the effect of one factor on the chemical reaction of an enzyme Background Information Globular proteins which are called enzymes are produced inside the human body. Enzymes are organic catalysts which mean they have the role of speeding up chemical reactions. The role of enzymes is essential for most human body functions. Specific enzymes at specific locations within the human body attain specific abilities to aid chemical reactions. [1] In this experiment, the type of enzyme that will be used for testing is called rennin. Rennin is a type of enzyme that can be found in the stomach of humans and is produced by the stomach cells of humans. Rennin's specific enzyme ability is to transform milk into semi-solid particles. If milk was not coagulated (solidified), milk would just simply flow through the stomach and not be 'chemically' digested. [2] Normally, the human body temperature is 37�C and rate of reaction is most favourable at this temperature. [2] As with all enzymes, altering the temperature affects the rate of rennin and milk. According to the principles of Kinetic Theory, increasing the temperature would increase speed of particle collision and therefore increase the rate of reaction. ...read more.

Middle

The tip of the spatula will be placed onto the milk. Gentle pressure will be put onto the spatula and if the milk is hard then the milk is therefore solid Materials * 15 � Test tube * Wooden tongs * 5 � Test tube rack * Permanent marker * 3 � Stop watches (� 0.01secs) * 0-110 �C Hot plate * 250cm3 beaker (� 25cm3) * 0-110�C Thermometer (� 0.01�C) * 10cm3 Measuring cylinder (� 0.01cm3) * Retort stand * 30cm3 of Rennin enzyme * Metal clamp * 100 cm3 of Pauls full cream milk * 10cm3 Volumetric pipette (� 0.01cm3) * 800cm3 distilled water * Plastic head for pipette * 2 � Metal spatula * 50cm3 Boiling tube Method Safety Precautions What Risk Safety Precautions Actions to take Attire Substance spill on clothes and skin Substance contact with eyes Burns from using hot plate Wear lab coats and ensure buttons are done up and sleeves are covering whole arm Wear goggles on at all time If substance spill onto the skin, wash it off immediately If substance gets into eyes, was eyes out using eye taps If skin is burnt due to contact with hot plate, leave it under cold running water for ...read more.

Conclusion

When the two substances are mixed, start timing on the stopwatch. 4. Continue step 2 - 3 for the remaining 2 test tubes which are labelled 25 5. Observe the physical state of the milk and record relevant qualitative data in 'Qualitative Data' table. When the milk nearly seems to be solid, use the other clean spatula to test if the milk is solid. Place the tip of the spatula onto the milk. Gentle put pressure on the spatula, if the milk is hard then the milk is solid. When the milk becomes solid, stop timing. 6. Record time shown on the stop watch onto the results table 7. Repeat steps 2 - 6 for the remaining independent variables - 30�C; 35�C; 40�C; 45�C. Ensuring that the temperature of the rennin enzyme is respective to the label of the beakers Note: If temperature of the enzyme is greater than what is needed for the specific test then remove boiling tube away from the hot plate - still ensuring thermometer is place inside it so that temperature can be observed Ensure that temperature of the milk is kept at 25�C for the whole experiment. This means, room temperature should not be adjusted during experiment as it might affect experiment results. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our International Baccalaureate Chemistry section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related International Baccalaureate Chemistry essays

  1. Viscosity lab. Research question:- How is the viscosity of water affected by ...

    the temperature of the water relative to the temperature of the burette, in order to help slowing down any temperature transfer to guarantee that water inside the burette will remain very hot.) - water that was used to heat the burette was replaced with new boiling water.

  2. IB chemistry revision notes

    needs to be positive. * Hydrogen Half-Cell The p.d. obtained when an electrode of some substance is placed in a 1M solution of it's ions to make a 1/2 cell and is used to complete a circuit using a hydrogen half-cell at S.T.P.

  1. Free essay

    Vitamin C in Fruit Juices

    Drop by drop, the iodine solution was added to the flask while swirling. The iodine was continually added and swirled, until the purple color was slow to go away. Then very carefully one drop was added and swirled. If the purple color disappeared within 15 seconds, another drop was added and swirled.

  2. How duration affects the rate of electrolysis in a Voltaic Cell

    - Blue colour of copper sulphate solution begins to get paler. - Zinc electrode begins to corrode a bit. Most corrosion can be observed at 35 minutes time interval. Note* - Uncertainties: The average reaction time was �0.5s even though it did alter from interval to interval.

  1. IB Chemistry Lab Design - compare the effect of temperature on the concentrations of ...

    Use the glass rod to stir. 1. Once it seems that the acid has fully dissolved, add some more water to the solution, to ensure that all the acid has actually dissolved. Then, transfer the solution into the 1000 ml standard flask using a washed funnel and the glass rod.

  2. The aim of this experiment is to examine the enthalpy of combustion of the ...

    the sequence of exothermic chemical reactions between a fuel and an oxidant accompanied by the production of heat and conversion of chemical specie ) of a primary alcohol increases . This means that as the number of carbon- carbon bonds increase, with the increase in the carbon chain length, more

  1. Measuring the fatty acid percentage of the reused sunflower oil after numerous times of ...

    5.479 ± 0.018% = 2.7% ± 0.2% ml/g oleic acid 24 ASLAN Özge Cemre D129077 Calculations for sample 4 (20 times frying): Trial 1: V = 19.0 ± 0.1 ml → Vε = 19.0 ± 0.5% ml m = 5.002 ± 0.001g → mε = 5.002 ± 0.020% g A% = (19.0 ± 0.5% x 0.0028 x 100)

  2. Investigating Factors that Affect the Rate of Reaction of the Decomposition of Hydrogen ...

    Turn on a computer and start the LoggerPro Program. Connect the Gas Pressure Senor to Channel 1of the Vernier computer interface and with the correct cable attach the Vernier computer interface to the computer. Take the plastic tubing with the Leur-lock connectors at either end of the tubing, connect the tubing to the base on the one-hole rubber stopper

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work