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Separation of Amino Acids by Thin Layer Chromatogrpahy

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Lab Report: Separation of Amino Acids by Thin Layer Chromatogrpahy Name: Klassa Andersen Lab-Colaborators:Marcus Jones Class: IB1 Data Collection and Processing: Table 1(the recordings of the samples): Test 1 (cm) (Uncertainty �0.5mm): Test 2 (cm) (Uncertainty �0.5mm): Sample 1(Unknown amino acid) : 0.25/1.3 0.25/1.4 Sample 2(alanine): 0.4 0.4 Sample 3(leucine): 1.3 1.4 Sample 4(lysine): 0.25 0.25 To find out what the Rf value is, we to find out the formula for it: The Y value represents the distance between point A and B and also between Point a and b. The X-value represents the numbers from the samples collected from the recordings of the samples. ...read more.


Based from our results, we see that Sample 1 is a mixture of leusine and Lysine since the Rf value of sample 1 is the same as sample 4. The theoretical results that are above are clearly conceded with the experimental results that we got in this experiment. Based from the Rf values we could see that the alanine is non-polar molecule with a basic PH value, leucine is a polar molecule with a basic and the lysine is a non-polar molecule with a neutral PH value. Certain variables that affect the Rf values, could be the polarity of the different amino acids. ...read more.


Also the amount of butanol in solvent putted into the amino acids can be a factor to if the molecules are polar or non-polar. Evaluation: The experiment went well, but the experiment could be improved. During the experiment, there were some human errors when checking the time and adding the concentrated polar solution off butanol. We didn't have the proper equipment to measure the precise measurements of the amino acids. This experiment could be improved by: 1. Repeating the experiment 2. Getting proper equipment for the experiments 3. Fewer human errors 4. Getting a more Un-polar solution of Butanol. Appendix: Picture 1(a image showing the results of the tests): ...read more.

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