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To check how the rate of transpiration is affected by a change in the PH of the soil/water.

Free essay example:

Aim

To check how the rate of transpiration is affected by a change in the PH of the soil/water.

Apparatus

1.) Hydrilla plant

2.) Test tubes

3.) Paraffin wax

4.) Beaker

5.) NaOH pellets

6.) HCl

7.) PH Paper

8.) Distilled water

9.) Stirrer

10.) Scissors

Procedure

  1. Prepare NaOH solution. Put a few pellets of NaOH in a beaker containing distilled water and stir until the pellets dissolve. This is the dil.NaOH which has a PH of 14. This is checked using the PH paper.
  2. Prepare dil. HCl. Take a beaker containing distilled water and pour 1-2 drops of HCl into it and stir. This gives dil.HCl which when checked with the PH paper gives PH value 1.
  3. Take 5 test tubes labeled a, b, c, d and e.
  1.  This is the control. Take 10ml of distilled water. Pour a drop of paraffin wax into the test tube as this will prevent the water from evaporating in the sunlight.
  2. Pour 10ml of the dil.NaOH into this test tube followed by a drop of paraffin wax.
  3. Pour 10ml of the dil.HCl into this test tube followed by a drop of paraffin.
  4. Take 10ml of tap water and pour into this test tube. The PH of the tap water is taken to be 5.5 as seen using the PH paper.
  5. Take 10ml of distilled water and pour into this test tube. It has a PH of 7 and is a neutral solution. Now we have 4 test tubes containing different PHs, rating all the way from 1(acidic) to 14(alkaline).
  1. Take the 4 hydrilla plants and using the scissors carefully trim the leaves such that all 4 weigh the same. Now carefully place them one by one in test tubes b, c, d and e.
  2. Now leave the 5 test tubes in a beaker in the sunlight for 24 hours.

Observations and Tabulation

  1. The weight of one test tube = 14.385g
  2. The weight of one plant = 1.735g
  3. The weight of the 10ml of liquid = 9.88g
  4. The weight of the test tube + liquid + plant = 26g
  5. The test tubes were put out in the sunlight on the terrace at 11a.m. for 24hrs.
  6. They were observed again at 11a.m. on the next day and these were the weights of the test tubes:
  1. 25.9g
  2. 23.8g
  3. 25.1g
  4. 24.6g
  5. 24.1g
  1. The weight of the control had remained almost the same, hence showing that since no transpiration had taken place, the water lost was negligible.
  2. The hydrilla in the test tube containing the NaOH had transpired the most. The percentage of water lost through transpiration was:

image00.png

  1. The hydrilla in the test tube containing the HCl had transpired the least. The percentage of water lost through transpiration was:

image01.png

  1. The hydrilla in the test tube containing the tap water had transpired a little lesser than the hydrilla in the distilled water. The percentage of water lost through transpiration was:

image02.png

  1. The hydrilla in the test tube containing the distilled water had transpired a bit more. The percentage of water lost through transpiration was:

image03.png

LIQUID

PH

% OF WATER LOST THROUGH TRANSPIRATION

Dil. HCl

1

3.46

Tap Water

5.5

5.38

Distilled Water

7

7.31

Dil. NaOH

14

8.46

image04.png

Conclusion and Evaluation

Taking the observations into consideration, we can conclude that as the PH of the water absorbed by the plant rose, the amount of water transpirated also rose considerably.

Looking back at the experiment there are a few ways in which it can be improved:

  1. Instead of using PH paper use a PH meter as the PH values will be much more accurate.
  2. Use more chemicals so that there can be different PH readings, for example, dilute and concentrated NaOH and HCl, different compounds can also be used so that the conclusion can be more accurate.

Rishikesh Dhoot        Page

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