• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Effect Of An Inhibitor Such As Copper Sulphate On The Activity Of Catalase

Extracts from this document...


Effect Of An Inhibitor Such As Copper Sulphate On The Activity Of Catalase The enzyme Catalase catalysis the decomposition of Hydrogen Peroxide to form smaller molecules: water and oxygen gas. In the reaction: 2H2O2 ------------> 2H2O + O2 The aim of this investigation is to find out how different concentration of Copper Sulphate (inhibitor) effect the activity of the enzyme Catalase. Hypothesis: As the concentration of Copper Sulphate increases the activity of Catalase will decrease and vice versa. In other word, at one extreme (high concentration) of CuSO4, the production of Oxygen will likely to stop completely and at the other extreme (low concentration) of CuSO4 the production of Oxygen is likely to continue with out no change. If the amount of inhibitor is fixed, then increasing the substrate concentration can reduce chances of inhibition by CuSO4. As two types of molecules are competing for the Catalase active site. The more H2O2 molecules there are, the less likely is it that CuSO4 (inhibitor) ...read more.


Research that I carried out on the internet, I found out that the Fe ion is found in the active site of yeast (Catalase). This means that Cu ions can displace the Fe ions (in enzyme) thus inhibit Catalase, and consequently disruption can be caused to the configuration of hydrogen bonds and the hydrophobic interaction which is holding the enzyme molecule. Method Part A: Apparatus * A conical flask to place the mixture of the enzyme, substrate and the inhibitor * A 5ml syringe to inject 2ml of yeast (catalase) enzyme into the mixture * A gas syringe to collect and measure the volume of Oxygen gas is produced within specific time interval * A clamp to hold the gas syringe * 13 test tubes 6 for different concentration of inhibitor and 6 for the substrate and 1 for water * 3 100ml measuring cylinders to measure out the volume of each variables * A stop clock to measure rate of production of Oxygen gas * A test tubes rack will be needed to hold the ...read more.


And see how much oxygen gas is collect within every five seconds of the reaction time * I will carry out each reaction for 60 seconds * Record the amount gas produced in my results table by reading of the gas syringe * I will repeat each experiment twice. This will be done to give an average volume of gas produce within the given time MY RESULT TABLE TUBES/TIME 10 20 30 40 50 60 A1 19 35 44 48 54 57 A2 21 35 46 49 54 58 AVE O2 CM3 20 35 45 48.5 54 57.5 B1 18 30 36 42 48 53 B2 19 32 36 42 48 55 AVE O2 CM3 18.5 31 36 42 48 54 C1 18 28 31 35 38 43 C2 19 26 32 35 38 41 AVE O2 CM3 18.5 27 31.5 35 38 42 D1 13 21 26 31 34 38 D2 12 22 27 31 33 38 AVE O2 CM3 12.5 21.5 26.5 31 33.5 38 E1 7 12 14 16 17 18 E2 7 12 14 16 17 19 AVE O2 CM3 7 12 14 16 17 18.5 Dublur Hussain Enzyme Coursework ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our University Degree Microbiology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related University Degree Microbiology essays

  1. The Effect of copper sulphate on the activity of catalase.

    are: * Change in temperature * Change in pH * Change in concentration of reagents Fair testing To make sure this experiment is a fair test; * the only factor I will vary will be the volume of water * the volume of yeast (catalase)

  2. Assess the effect of adjacent pH on the germination of a mustard seed.

    I do not know why, as is possible to see from my graph, that far more germination took place in water than in any of the other solutions. The molecule size difference would not have applied unless the sites where osmosis occurs may be different sizes, or water molecules are

  1. Microbiology coursework

    Once the microorganism is in a pure culture, diagnostic tests (such as Gram staining) can be run to identify the bacteria as was done in this practical. The method of culturing microorganisms is by streaking on agar plates. Since B.

  2. Introduction to Spectrophotometric Analysis - By using spectrophotometric analysis or spectrophotometry, one can determine ...

    Some amino acids which are having aromatic side chains especially tyrosine and tryptophan absorb most of the light in the UV region where the wavelength is lower than 300nm (Gore, 2000). From the protein spectrum obtained above, the wavelength of absorption is at 229nm and 279nm, both are lower than

  1. The Effect of pH on the Rate of Catalase

    At the lowest pH value the reaction rate was low. At the low pH values there were many more H+ ions in the solution these interacted with the bonds in the enzyme. The enzyme was denatured. It altered the structure of the active site and changed the charge, this would

  2. Investigation into the effect of Temperature on the action of the Enzyme Lipase.

    In other words at higher temperatures bonds are broken which mean that the lipase enzyme key can no longer fit in the milk substrate lock (diagram of lock and key hypothesis is show below as diagram A). This process is called denaturing.

  1. Observe the effect of temperature on activity of the enzyme amylase.

    After this there is a slight fall from 40 to 50oC. From 50oC onwards, there is a sharp drop. The peak on the best fit line shows the temperature where the enzyme was at its highest activity. This is around 40oC.

  2. Determine the amount of gas evolved by the fermentation of different sugars by yeast.

    the sugar solution in different test tubes, to each tube 2ml of yeast suspension and then the tubes were brought up with water. 3. Each fermentation tube was inserted in the larger tube as far it could go and then it was inverted.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work