• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Experiment on Spectrophotometer

Extracts from this document...


Experiment on Spectrophotometer Determination of Cytochrome C: Preparing a Calibration Graph Introduction This is an experiment to determine the concentration of a colored compound in a solution by comparison of its light absorption with the light absorption of a standard solution of a known solution. This experiment will be done by using spectrophotometer, as it will determine the amount of light that will be absorbed by the solution. Also by using the Gilson pipette it enables you to accurately make sure solutions have been measured correctly, also when using the Gilson pipette you should change the tips to stop contamination of the solution. ...read more.


(ABS) 380 0.285 385 0.307 390 0.380 395 0.479 400 0.608 405 0.681 410 0.606 415 0.423 420 0.255 The table above shows the results for the determination of peak wavelength. Tube No. 1 2 3 4 5 6 Stock Solution (0.1mg/ml) (ml) 0 2 4 6 8 Deionized Water (ml) 10 8 6 4 2 0 Final Concentration [cytc] (mg/ml) 0 0.02 0.04 0.06 0.08 1 ABS at peak = 405 nm 0 0.012 0.220 0.281 0.649 0.661 Corrected ABS (-blank) 0 0.012 0.220 0.281 0.649 0.661 The above information shows the results of the Stock Cytc Solution 0.1 mg/ml. ...read more.


shows me that there is a rise in absorption until it hits the peak at 405 nm, and then there is a sudden drop in absorption. The results of the calibration of cytochrome c show me that it goes up by each test tube, as more ml of stock solution is used. However, the results should show on the graph as a straight line, in this case there must have been an error or the solution was not prepared and measured correctly. The sources of error show a 4%. Overall the experiment was a success as the objectives were met and the absorption of the known solution were recorded. ?? ?? ?? ?? ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our University Degree Applied Biology section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related University Degree Applied Biology essays

  1. Lab report: food dehydration. This experiment focuses on the follow four questions: ...

    The following figure 1 shows the level of water activity and correspondent degradative reactions rates and microbial growth limits. Figure 1 the level of water activity and correspondent degradative reactions rates and microbial growth limits Source: http://www.aqualab.com/education/water-activity-for-product-safety-and-quality/ Based on this figure, it can be concluded that low water activity can

  2. This experiment was carried out to separate and characterize the protein mixture which contained ...

    This indicated that the isoelectric point of serum albumin is lower than pH6. Hua et al. (2008) claimed that the isoelectric point of serum albumin is 4.8. This showed that the result obtained in this experiment was consistent with the theoretical result.

  1. Isolation & Characterisation of Proteins. The purpose of conducting this experiment is to ...

    As a conclusion from the gel image shown in figure 1, for sample A, B and C, the first band, which is 8.497kDa, 8.135kDa and 7.788kDa respectively, shows the experimental molecular weight of haemoglobin. As for the second band in sample A, B and C are 65.892kDa, 63.083kDa and 60.392kDa respectively which are the experimental molecular weight for serum albumin.

  2. SDS-PAGE and Western Blotting Lab report (extensive methods section)

    Western Blotting When the gel was remobed from the glass, it was soaked in transfer buffer, whilst 2 fibre pads were also saturated in buffer.

  1. Superbugs the rise and fall of hospital acquired infections

    MRSA was originally treated by the antibiotic penicillin however the strain become resistant to it just 4 years after the drug was mass produced. This lead to the use of glycopeptide antibiotics, such as vancomycin. However in the late 1990s treatment started to fail because some strains acquired decreased susceptibility to vancomycin, they had intermediate levels of resistance (4-8ug/ml).

  2. The control of information in Science - A One World Essay on Biotechnology.

    Along with supposedly having large amounts of biological weapons in their country, Iraq also has another biological weapon that is just as toxic, botulinum toxin. (No author, www.ornl.gov). If botulinum toxin (more commonly called Botox) appears in large scales in the human body, the effect could be deadly.

  1. Food Science -Experiments to Determine the Properties and Uses of Enzymes in Food Preperation.

    Much of the tenderizing effect is the result of the enzyme destroying the sarcolemma surrounding the myofibrils in the fibers, hydrolyzing actomyosin, and then continuing hydrolytic breakdown of various proteins in the fiber. Collagen also may be hydrolyzed to contribute still further to the tenderizing effect.

  2. EFFECT OF Light wave Length on Photosynthisis

    With the assistance of ATP (product of light reaction) and another chemical called NADPH, the carbon dioxide is turned into sugar.( CALVIN, M. 1962 ). Aim: The aim of this experiment is to determine whether changing the light colour /intensity can affect photosynthesis.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work