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Experiment on Spectrophotometer

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  • Essay length: 508 words
  • Submitted: 11/08/2009
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University Degree Applied Biology

The first 200 words of this essay...

Experiment on Spectrophotometer

Determination of Cytochrome C: Preparing a Calibration Graph

Introduction

This is an experiment to determine the concentration of a colored compound in a solution by comparison of its light absorption with the light absorption of a standard solution of a known solution.

This experiment will be done by using spectrophotometer, as it will determine the amount of light that will be absorbed by the solution. Also by using the Gilson pipette it enables you to accurately make sure solutions have been measured correctly, also when using the Gilson pipette you should change the tips to stop contamination of the solution.

Objectives

o Learn to use a spectrophotometer

o Determine the degree of light absorption by a colored compound

o Plot a spectrum of light absorption against wavelength is best to use for peak wavelength

o Make a standard solution of known concentration, and then plot them to a graph

o Understand calibration graphs

o Measure the concentration using a graph

o Writing a experimental report

Method

Refer to manual

Results

(Nm) (ABS)

380 0.285

385 0.307

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