Identifying microorganisms using differential staining techniques

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IDENTIFYING MICROORGANISM'S USING DIFFERENTIAL STAINING TECHNIQUES

Introduction:

The purpose of this lab is to experience preparing slides and drawing cells that involve the process of differential staining. In this lab we will be using five different microorganisms. In experiment 4 we will use Staphylococcus epidermidis and Escherichia coli. Staphylococcus epidermidis is a gram-positive cocci shaped bacteria that is known to be one of the leading causes of infections, particularly the foreign body infections (Bukhari, 2004). On the other hand, Escherichia coli is a gram-negative rod shaped bacteria that is known to produce and grow in the intestines of animals and humans often from the ingestion of foods or water (Todar, 2008). In experiment 5 we will use Mycobacterium phlei. M. phlei is a gram-positive cocci shaped bacteria that is of the genus Mycobaterium (SteadyHealth.com, 2004). This type of bacteria is one of the hardest to destroy because of the mycolic acids that cover the cell bodies (SteadyHealth.com, 2004). Also, this bacteria is often the cause of human infections but people infected often respond well to anti-mycobacterial therapy (SteadyHealth.com, 2004). In experiment 6 we will use Bacillus megaterium and Clostridium sporogenes. Bacillus megaterium is a gram-positive rod shaped bacteria that