Isolation & Characterisation of Proteins. The purpose of conducting this experiment is to study protein separation using two different methods, which are the SDS-polyacrylamide gel and ion exchange chromatography

The first 200 words of this essay...

Name: Ng Yen Pheng

Student ID: 22353046

Day and date: Tuesday, 3 April 2012

Title: Isolation & Characterisation of Proteins

Aim:

The purpose of conducting this experiment is to study protein separation using two different methods, which are the SDS-polyacrylamide gel and ion exchange chromatography. This practical also aims to study what types of ion exchangers which are more suitable to separate different types of proteins. This experiment also aims to compare the separation of proteins by SDS-PAGE and ion exchange chromatography.

Results:

Part A: SDS-PAGE

Figure 1: Image of SDS-Polyacrylamide gel electrophoresis.

Table 1: Distance migrated (cm) by different length of protein fragments denatured by SDS

Bands

Molecular Weight, MW (kDa)

Log10 (MW)

Distance migrated (mm)

1

250

2.40

14

2

130

2.11

24

3

100

2.00

33

4

70

1.85

43

5

55

1.74

48

6

35

1.54

-

7

25

1.40

66

8

15

1.18

75

9

10