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Mast Cell Degranulation Practical

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´╗┐Mast Cell Degranulation Practical ________________ 1. I can observe from the class results that the untreated cells (The control group) had highest percentage of cells that gave a degranulation score of 0 (53.62) when compared to cells that have been treated with 48/80 (23.68), SCG (48.08) and Salbutamol (36.48). I did predict that the control cells would have had a score higher than those treated with 48/80 but I expected the cells treated with SCG and Salbutamol to have a higher percentage of cells that gave a score of 0 compared to the control. After having done research, I found out that there are endogenous factors that also cause degranulation and treating the cells with SCG or Salbutamol would not inhibit this process that has already started (Shen, W. ...read more.


It did not however agree with my prediction regarding the percentage of partially degranulated cell, since the percentage the class recorded was higher than that of the non-degranulated cells. I believe this is mainly due to human error in determining which cells are degranulated and those that aren?t. It might also be due to endogenous factors that caused the cells to start degranulation as stated previously. 3. The results obtained from the class is that 48/80 and SCG treated cells have a higher percentage of partially degranulated (42.36) and fully degranulated (25.62) cells compared to the cells that were treated with Salbutamol and 48/80 which gave a percentage of 40.86 and 19.65 percent respectively. ...read more.


et al. 2000). IP3 then binds to receptors that are ligand-gated Ca2+ channels. The Influx of Calcium ions then causes exocytosis of the granules (Widmaier, E. et al. 2008) (Rang, H. P. et al. 2012). Reference: SHEN, W. and LOUISE, S. G. (1999). Immunology for pharmacy students. Amsterdam: Harwood Academic. Joint Formulary Committee (2011). British National Formulary. 61st edition. London: British Medical Association and Royal Pharmaceutical Society of Great Britain. WIDMAIER, E. et al. (2008). Vander?s Human Physiology. 11th edition. New York: McGraw-Hill. RANG, H. P. et al. (2012). Rang and Dale?s Pharmacology. 7th edition. London: Elsevier. CHAHDI, A. et al. (2000) Compound 48/80 Activates Mast Cell Phospholipase D via Heterotrimeric GTP-Binding Proteins. The Journal of Pharmacology and Experimental Therapeutics, 292 (1), pp. 122- 130. ________________ Ahmed Loyan Abdi Ali P10525592Page ...read more.

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