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Microscope observation of animal and plant cells

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Introduction

Microscope observation of animal and plant cells Introduction Cells represent the make up of our being as well as many other organisms whether they are unicellular or multicultural. Cells can also be complex or quite simplistic in design and contents, depending on the purpose of its function in an organism. Typically when studying cells some form of magnifications must be used to see the finest details of the cell, microscopes are used due to constraints in human eyes that only allow us to see to so much. In essence their use is to see what the eye cannot. This may include processes such as mitosis, phagotycosis and other forms of transport within the cell. ...read more.

Middle

Cheek cell Onion Stomata. All of the specimens where viewed under x100 magnification, this is achieved by the lenses on the eye piece producing x10 and then the objective lenses producing an additional x10 with in turn give x100 magnification. Discussion The focus of this practical involved making use of epithial tissue in animals and surface tissue in plants. There are different types of epithial cells the ones from the cheek cell are squamous epithelial cells. They form the epithial tissues that are continuous sheets, which cover most structures or cavities within the body.i Due to the limitations of the light microscopes, we are only able to observe some of the organelles within the 3 structures we viewed. ...read more.

Conclusion

Parts of the cell that's are affected by eosin include the cytoplasm, because of its basic nature. Haematoxylin is not really regarded as an actually a dye although this is debatable by some, where as eosin is an acid dye. Haematoxylin is able to develop colour-like properties when oxidized.iii Because of their different properties they both sow their importance in staining because of their characteristics. As stated within the practical organelles were limited to what could be seen with the light microscope. An electron microscope would have highlighted organelles such as Golgi body, mitochondria etc. in three dimensions. Reference: i Barbour, M. et al. (1997) Biology. London: Collins Educational ii http://protocolsonline.com/histology/haematoxylin-eosin-he-staining/ accessed on 17/11/2010 iii http://www.lab.anhb.uwa.edu.au/mb140/MoreAbout/stains.html accessed on 17/11/2010 Dominic Areago ...read more.

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