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The Effect Of Osmosis In Animal Cells, Plant Cells & A Model System

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Introduction

The Effect Of Osmosis In Animal Cells, Plant Cells & A Model System Introduction The purpose of this experiment is to determine as well as observe the movement of water molecules going in and coming out of model system, animal cell and plant cell through a process called osmosis. Osmosis is referred to the movement of water molecules through a semi-permeable cell membrane from an area of low solute concentration to an area of high solute concentration within a biological cell system (Karp, 2010). In other terms osmosis can also be referred to as the movement of water molecules through a cell membrane from an area of high water concentration to an area of low water concentration (Karp, 2010). In this lab the membrane of the cells that are tested are permeable to water molecules, but impermeable to solutes. Permeability refers to substances that can enter, while impermeability means that substances can not enter (Karp, 2010). Within this experiment when the process of osmosis is observed between two solutions, the solute concentrations between the two solutions are compared in either three ways; they can either be isotonic, hypertonic or hypotonic. An isotonic solution is a solution that has the same concentration of solute to the solution being compared with (Bowen, 2010). ...read more.

Middle

interval 25 min. interval 30 min. interval 35 min. interval 40 min. interval 1 0.5g 0.4g 0.1g -0.1g -0.2g -0.3g -0.4g -0.5g 2 1.3g 2.0g 2.3g 2.4g 2.5g 2.8g 3.1g 3.4g 3 2.7g 3.3g 4.0g 4.6g 5.1g 5.8g 6.4g 7.0g 4 -1.1g -1.5g -1.8g -1.9g -2.3g -2.6g -2.9g -3.0g 5 -0.8g -2.1g -3.2g -3.9g -4.3g -5.0g -5.4g -5.9g This table compares the change in weight of each dialysis bag at every interval with respect to the different concentration of solution inside the bag and the concentration of fluid surrounding the bag within each beaker. Overall, the change in weight is consistent over each time interval for each trial. Figure 1. Graph showing the trend of the change in weight of each dialysis bag over each time interval This graph is a visual representation of the data presented in table 2 showing the change in weight of each dialysis bag over each time interval. As one can see, bag #2 and #3 have a positive slope and bag #1, #4, and #5 have a negative slope. Overall, the change in weight indicates a linear trend. Table 3. Initial observations showing the difference between isotonic, hypertonic and hypotonic mammalian blood solutions Type of Fluid Solution Observations of hematocrit Observations of flask Observations of slide isotonic - hematocrit is 13%; plasma is 87% - colour of solution is plain red, the solution is not too dark and not too light - orangish-red cells that are spaced apart and not packed. ...read more.

Conclusion

On the other hand, the red blood cells in the hypertonic solution have shrunk, because the amount of water molecules that have exited the cells was more than the normal amount ("Osmosis: Background," ). The red blood cells in the isotonic solution have stayed the same because their is no net movement of water molecules entering or leaving the cells ("Osmosis: Background," ). Looking at the results from part 4 of this experiment it can be concluded that in a hypertonic environment the vacuole in the cell leaf starts to shrink and become plasmolyzed because water molecules are exiting the vacuole during osmosis (Department of Biology, 2011). In a hypotonic environment, the vacuole remains fairly the same size as it would be in an isotonic environment because the cell wall of the leaves prevents the vacuole from rupturing by not letting the vacuole receive extra water (Department of Biology, 2011). In the isotonic environment the vacuole is not to large and not to small because there is no net movement of water entering or exiting the vacuole; instead the vacuole is filled with sap (Department of Biology, 2011). From all three parts of the experiment the effect that an isotonic, hypertonic, and hypotonic solution have on a cells is evident through the observations. ...read more.

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